Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor

Kenswil, Keane Jared Guillaume; Jaramillo, Adrian Christopher; Ping, Zhen; Chen, Si; Hoogenboezem, Remco M.; Mylona, Maria Athina; Adisty, Maria N.; Bindels, Eric; Bos, P.K.; Stoop, Hans; Lam, King H.; Eerden, Bram van der; Cupedo, Tom; Raaijmakers, Marc H.G.P.

doi:10.1016/j.celrep.2017.12.070

Cited by 46 publications

(51 citation statements)

References 69 publications

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“…Mesenchymal stem cells (MSCs) and endothelial cells (ECs) are the two major types of cells in bone marrow niche. It has been reported that ECs provide a nutrient-rich hematopoietic niche for HSC, which can effectively promote HSC proliferation and differentiation [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

et al. 2020

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Background: The present study investigated the effects of microvascular endothelial cells (MECs) on the chemotaxis, adhesion and proliferation of bone marrow hematopoietic stem cells (HSCs) ex vivo. Methods and Results: MECs were collected from the lung tissue of C57BL/6 mice, and HSCs were isolated with immunomagnetic beads from bone marrow of GFP mice. MECs and HSCs were co-cultured with or without having direct cell–cell contact in Transwell device for the measurement of chemotaxis and adhesion of MECs to HSCs. Experimental results indicate that the penetration rate of HSCs from the Transwell upper chamber to lower chamber in ‘co-culture’ group was significantly higher than that of ‘HSC single culture’ group. Also, the HSCs in co-culture group were all adherent at 24 h, and the co-culture group with direct cell–cell contact had highest proliferation rate. The HSC number was positively correlated with vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) levels in supernatants of the culture. Conclusions: Our study reports that MECs enhance the chemotaxis, adhesion and proliferation of HSCs, which might be related to cytokines SDF-1 and VEGF secreted by MECs, and thus MECs enhance the HSC proliferation through cell–cell contact. The present study revealed the effect of MECs on HSCs, and provided a basis and direction for effective expansion of HSCs ex vivo.

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Section: Introductionmentioning

confidence: 99%

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

et al. 2020

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“…(44)(45)(46) Interleukin-33 (IL-33) produced by a subset of CD105 expressing ECs in bone marrow facilitate osteogenesis and hematopoiesis. (47)…”

Section: Pattern and Diversity Of Blood Vessels In Bonementioning

confidence: 99%

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

Chen

Hendriks

Chatzis

et al. 2020

Journal of Bone and Mineral Research

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Bone vasculature and bone marrow vascular niches supply oxygen, nutrients, and secrete angiocrine factors required for the survival, maintenance, and self-renewal of stem and progenitor cells. In the skeletal system, vasculature creates nurturing niches for bone and blood-forming stem cells. Blood vessels regulate hematopoiesis and drive bone formation during development, repair, and regeneration. Dysfunctional vascular niches induce skeletal aging, bone diseases, and hematological disorders. Recent cellular and molecular characterization of the bone marrow microenvironment has provided unprecedented insights into the complexity, heterogeneity, and functions of the bone vasculature and vascular niches. The bone vasculature is composed of distinct vessel subtypes that differentially regulate osteogenesis, hematopoiesis, and disease conditions in bones. Further, bone marrow vascular niches supporting stem cells are often complex microenvironments involving multiple different cell populations and vessel subtypes. This review provides an overview of the emerging vascular cell heterogeneity in bone and the new roles of the bone vasculature and associated vascular niches in health and disease.

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“…Using mice expressing a citrine reporter in place of the Il33 gene, we found these CD169 + macrophages were the most abundant IL-33 producers in the BM (Fig. 4 D), exceeding the numbers of IL-33 + Ter119 + and CD45 − stromal cells described previously (Kenswil et al, 2018;Wei et al, 2015).…”

Section: Il-33 Is Increased In the Bm Niche With Inflammationmentioning

confidence: 63%

IL-33 promotes anemia during chronic inflammation by inhibiting differentiation of erythroid progenitors

Swann

Koneva

Regan-Komito

et al. 2020

Journal of Experimental Medicine

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An important comorbidity of chronic inflammation is anemia, which may be related to dysregulated activity of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow (BM). Among HSPCs, we found that the receptor for IL-33, ST2, is expressed preferentially and highly on erythroid progenitors. Induction of inflammatory spondyloarthritis in mice increased IL-33 in BM plasma, and IL-33 was required for inflammation-dependent suppression of erythropoiesis in BM. Conversely, administration of IL-33 in healthy mice suppressed erythropoiesis, decreased hemoglobin expression, and caused anemia. Using purified erythroid progenitors in vitro, we show that IL-33 directly inhibited terminal maturation. This effect was dependent on NF-κB activation and associated with altered signaling events downstream of the erythropoietin receptor. Accordingly, IL-33 also suppressed erythropoietin-accelerated erythropoiesis in vivo. These results reveal a role for IL-33 in pathogenesis of anemia during inflammatory disease and define a new target for its treatment.

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Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor

Cited by 46 publications

References 69 publications

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

IL-33 promotes anemia during chronic inflammation by inhibiting differentiation of erythroid progenitors

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