Characterization of extended-spectrum β-lactamases and integrons in Escherichia coli isolates in a Spanish hospital

Seagulls of the Berlengas Natural Reserve of Portugal as Carriers of Fecal Escherichia coli Harboring CTX-M and TEM Extended-Spectrum Beta-Lactamases

“…The sequence of the fragment obtained by PCR upstream of the bla CTX-M-1 gene in the E. coli GV-23 strain revealed the presence of a region of the IS26 transposase flanking a partially truncated ISEcp1 followed by an intergenic region; this whole structure has been previously found in E. coli (13,22). The presence of ISEcp1 and IS903 surrounding the bla CTX-M-14a gene in E. coli GV-10 was identified, and the genetic environment of the bla CTX-M-32 gene detected in E. coli GV-12 included ISEcp1/IS5 upstream of the bla gene and orf477 downstream, as also detected by others (25). …”

Molecular Characterization of Extended-Spectrum β-Lactamase-Producer Klebsiella pneumoniae Isolates Causing Neonatal Sepsis in Peru

“…Detection of bla SHV , bla TEM , bla OXA-1-like , bla CTX-M-2G , bla CTX-M-1G , bla CTX-M-9G , and bla CTX-M-8G genes were performed by conventional polymerase chain reaction (PCR). [19][20][21] To determine the specific β-lactamase gene involved in the ESBL phenotype, 37 isolates were randomly selected for further sequencing of their PCR products on both strands (with the exception of bla OXA-1-like amplicons). To obtain the whole sequences of the bla CTX-M genes, their genetic environments were analyzed by specific PCRs and DNA sequencing.…”

“…To obtain the whole sequences of the bla CTX-M genes, their genetic environments were analyzed by specific PCRs and DNA sequencing. 19 The obtained sequences were aligned and compared with those included in Lahey (http://www.lahey.org/Studies/) and GenBank databases (http://www.ncbi.nlm.nih.gov/BLAST). Phylogenetic groups were studied by PCR restriction fragment length polymorphism.…”

Faecal Escherichia coli isolates from healthy dogs harbour CTX-M-15 and CMY-2 β-lactamases

“…Isolates that were positive by PCR with cit primers were examined for the presence of blaCMY-2 by PCR and sequencing (Vinué et al, 2008). Mutations in the promoter-attenuator region of the chromosomal ampC gene of E. coli were studied in cefoxitin and amoxicillin-clavulanic acid resistant E. coli isolates by PCR and sequencing.…”

“…Mutations in the promoter-attenuator region of the chromosomal ampC gene of E. coli were studied in cefoxitin and amoxicillin-clavulanic acid resistant E. coli isolates by PCR and sequencing. Sequences were compared with the same region of the E. coli K12 ampC gene to analyse mutations associated with overexpression of the ampC gene (Caroff et al, 2000;Vinué et al, 2008).…”