Characterization of Fluorescence Lifetime of Photofrin and Delta-Aminolevulinic Acid Induced Protoporphyrin IX in Living Cells Using Single- and Two-Photon Excitation

Russell, Jennifer; Diamond, Kevin R.; Collins, Tony; Tiedje, H.F.; Hayward, Joseph E.; Farrell, Thomas J.; Patterson, Michael S.; Fang, Qiyin

doi:10.1109/jstqe.2007.912896

Cited by 36 publications

(41 citation statements)

References 34 publications

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“…5-ALA is an endogenous heme precursor that can lead to phototoxic PpIX synthesis through ferrochelatase inside the mitochondria. [70]. The fluorescence lifetimes of both drugs in methanol or cells agreed well with previous studies [31,43,45], as summarized in Table 4.…”

Section: Binding To Biomoleculessupporting

confidence: 89%

“…TRF studies relevant to different PDT photosensitizers have been reported by several research groups in the past decades [31,43,45,[70][71][72][73][74][75][76]. FDA-approved PDT photosensitizers include Photofrin ® , 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX), and verteporphyrin, Other photosensitizing agents in research stage, such as mTHPC (m-tetrahydroxyphenylchlorin, Foscan ® ), phthalocyanine (Pc) compounds, and chlorin-based compounds (i.e., HPPH), also showed promising results in terms of their singlet oxygen yield and more red-shifted absorption bands [77].…”

Section: Time-resolved Studies Of Pdt Photosensitizers In Solution Anmentioning

confidence: 99%

“…How these factors affect lifetime measurements of the photosensitizers will be further discussed. [43] 10 ns (Organic solution) [70] 5.5 ns (Mitochondria, MLL) [70] 13.3 ns (Monomer, mitochondria) [70] 13.6 ns (Monomer, mitochondria) [45] 8.5 ns (Aggregates, mitochondria) [45] 8.0 ns (Aggregates, mitochondria) [78] 4.8 ns (Cell membrane) [78] 1.0 ns (Aggregates, mouse model) [31] 13 ns (Monomers, mouse model) [31] 5 µg/mL 0.06-6 µg/mL 7.6 ns (liposome confined) [74] 6.4 ns (tissue phantom) [75] 4.3 ns (mouse tumor, before PDT) [75] 5.0 ns (mouse tumor, after PDT) [75] …”

Section: Time-resolved Studies Of Pdt Photosensitizers In Solution Anmentioning

confidence: 99%

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Time-Resolved Fluorescence in Photodynamic Therapy

et al. 2014

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Photodynamic therapy (PDT) has been used clinically for treating various diseases including malignant tumors. The main advantages of PDT over traditional cancer treatments are attributed to the localized effects of the photochemical reactions by selective illumination, which then generate reactive oxygen species and singlet oxygen molecules that lead to cell death. To date, over-or under-treatment still remains one of the major challenges in PDT due to the lack of robust real-time dose monitoring techniques. Time-resolved fluorescence (TRF) provides fluorescence lifetime profiles of the targeted fluorophores. It has been demonstrated that TRF offers supplementary information in drug-molecular interactions and cell responses compared to steady-state intensity acquisition. Moreover, fluorescence lifetime itself is independent of the light path; thus it overcomes the artifacts given by diffused light propagation and detection geometries. TRF in PDT is an emerging approach, and relevant studies to date are scattered. Therefore, this review mainly focuses on summarizing up-to-date TRF studies in PDT, and the effects of PDT dosimetric factors on the measured TRF parameters. From there, potential gaps for clinical translation are also discussed. OPEN ACCESSPhotonics 2014, 1 531

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Section: Binding To Biomoleculessupporting

confidence: 89%

Section: Time-resolved Studies Of Pdt Photosensitizers In Solution Anmentioning

confidence: 99%

Section: Time-resolved Studies Of Pdt Photosensitizers In Solution Anmentioning

confidence: 99%

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Time-Resolved Fluorescence in Photodynamic Therapy

et al. 2014

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“…34,54,55 As such, changes in fluorophore lifetime can be potential noninvasive biomarkers for investigating and visualizing the local microvascular changes, photophysical processes, and photochemical reactions at molecular and cellular levels. [55][56][57][58][59] For example, microscopic TD imaging has been applied for studying photobleaching and lifetime dynamics of PDT photosensitizers in cells. 55,56,60,61 In this paper, we report the application of TD-FDOT for 3-D reconstructions of the fluorescence yield and lifetime of the photosensitizer HPPH in vivo before and after PDT.…”

Section: Introductionmentioning

confidence: 99%

“…[55][56][57][58][59] For example, microscopic TD imaging has been applied for studying photobleaching and lifetime dynamics of PDT photosensitizers in cells. 55,56,60,61 In this paper, we report the application of TD-FDOT for 3-D reconstructions of the fluorescence yield and lifetime of the photosensitizer HPPH in vivo before and after PDT. We first describe a TD-FDOT system, which employs an ultra-short pulse laser, gated imaging, and time-delay techniques.…”

Section: Introductionmentioning

confidence: 99%

Imaging a photodynamic therapy photosensitizerin vivowith a time-gated fluorescence tomography system

Rohrbach

Sunar

2012

J. Biomed. Opt

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Abstract. We report the tomographic imaging of a photodynamic therapy (PDT) photosensitizer, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) in vivo with time-domain fluorescence diffuse optical tomography (TD-FDOT). Simultaneous reconstruction of fluorescence yield and lifetime of HPPH was performed before and after PDT. The methodology was validated in phantom experiments, and depth-resolved in vivo imaging was achieved through simultaneous three-dimensional (3-D) mappings of fluorescence yield and lifetime contrasts. The tomographic images of a human head-and-neck xenograft in a mouse confirmed the preferential uptake and retention of HPPH by the tumor 24-h post-injection. HPPH-mediated PDT induced significant changes in fluorescence yield and lifetime. This pilot study demonstrates that TD-FDOT may be a good imaging modality for assessing photosensitizer distributions in deep tissue during PDT monitoring.

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Widefield fluorescence lifetime imaging of protoporphyrin IX for fluorescence‐guided neurosurgery: An ex vivo feasibility study

Erkkilä

Bauer

Hecker‐Denschlag

et al. 2019

Journal of Biophotonics

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Achieving a maximal safe extent of resection during brain tumor surgery is the goal for improved patient prognosis. Fluorescence‐guided neurosurgery using 5‐aminolevulinic acid (5‐ALA) induced protoporphyrin IX has thereby become a valuable tool enabling a high frequency of complete resections and a prolonged progression‐free survival in glioblastoma patients. We present a widefield fluorescence lifetime imaging device with 250 mm working distance, working under similar conditions such as surgical microscopes based on a time‐of‐flight dual tap CMOS camera. In contrast to intensity‐based fluorescence imaging, our method is invariant to light scattering and absorption while being sensitive to the molecular composition of the tissue. We evaluate the feasibility of lifetime imaging of protoporphyrin IX using our system to analyze brain tumor phantoms and fresh 5‐ALA‐labeled human tissue samples. The results demonstrate the potential of our lifetime sensing device to go beyond the limitation of current intensity‐based fluorescence‐guided neurosurgery.

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Characterization of Fluorescence Lifetime of Photofrin and Delta-Aminolevulinic Acid Induced Protoporphyrin IX in Living Cells Using Single- and Two-Photon Excitation

Cited by 36 publications

References 34 publications

Time-Resolved Fluorescence in Photodynamic Therapy

Time-Resolved Fluorescence in Photodynamic Therapy

Imaging a photodynamic therapy photosensitizerin vivowith a time-gated fluorescence tomography system

Widefield fluorescence lifetime imaging of protoporphyrin IX for fluorescence‐guided neurosurgery: An ex vivo feasibility study

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