Characterization of four isolates of Bombyx mori nucleopolyhedrovirus

Hong, Hee‐Jeon; Woo, Soo-Dong; Choi, Jae Young; Lee, H. K.; Kim, M. H.; Je, Yeon Ho; Kang, Seok Kwon

doi:10.1007/s007050070025

Cited by 27 publications

(19 citation statements)

References 20 publications

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“…The BmMNPV polyhedron ranges from 2.5 to 4.5 µm in diameter and has a truncated octahedron geometric shape (Torquato et al, 2006b). Hong et al (2000) suggest the existence of genetic variation in the polyhedron geometric shape of BmNPV and that this is specific for each viral lineage. Katsuma et al (1999) found variations in the shape of polyhedron of the BmNPV caused by polyedrin mutations.…”

Section: Resultsmentioning

confidence: 95%

“…The viral p10 protein, present in the polyhedral matrix, is involved in the nuclear and cell lysis (Hong et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

“…Palhan and Gopinathan (1996) had characterized a geographic viral isolate in Bangalore, india, with indications of being the multiple type. According to the literature, this viral subgroup is more virulent, and there is evidence for geographic viral variability to NPVs of the same species, where this variability could affect virulence (Adams and McClintock, 1991;Hong et al, 2000;Fan et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Brancalhão

Torquato²,

Fernandez³

2009

Genet. Mol. Res.

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ABSTRACT.A multiple nucleopolyhedrovirus previously isolated from infected Bombyx mori L. larvae (BmMNPV) in Paraná State, Brazil, was inoculated into B. mori larvae to examine susceptibility and cytopathology in silk gland cells. The anterior, middle and posterior silk glands were removed from the infected silkworm at different times post-inoculation and processed for cytopathology studies by light and transmission electron microscopy. BmMNPV infection was only detected at 72 h post-inoculation in cells of the middle and posterior silk glands. No sign of infection was found in the anterior silk gland. Cytopathological characteristics were similar to those found in other target tissues; initially, they consisted of nuclear hypertrophy and the formation of virogenic stroma (viroplasm), in which the progeny virions are produced. The virions are then enveloped and occluded in protein crystal structures, the polyhedra. After viral replication, cells undergo lysis and release a great number of BmMNPV polyhedra into the hemocoele. Histopathology showed early infection foci in regions surrounding trachea insertions, demonstrating that trachea is an infection-spreading organ in the insect body. Trachea penetrates the middle and posterior silk gland basal lamina, considered a barrier to virus, facilitating the penetration of budded virus. The anterior silk gland does not have tracheal insertions into the basal lamina, which reduces budded virus infection. Tracheal branches provide a conduit for non-occluded virions (budded virus) to pass through the basal lamina barrier and disseminate BmMNPV in the silkworm silk gland.

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Section: Resultsmentioning

confidence: 95%

“…The viral p10 protein, present in the polyhedral matrix, is involved in the nuclear and cell lysis (Hong et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Brancalhão

Torquato²,

Fernandez³

2009

Genet. Mol. Res.

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“…The analysis of BmMNPV ORF10 protein sequence showed no difference in the amino acid sequence of the BmNPV isolate K4 (Hong et al, 2000), BmNPV isolate T3 (Gomi et al, 1999), BmNPV Zhenjiang strain (Jiang et al, 2008), and BmNPV Xuwen strain (Jiang et al, 2008). The number of coded amino acids for p10 gene could vary among different isolates of nucleopolyhedrovirus and in different species of Lepidoptera.…”

Section: Bmmnpv Orf10 and Orf14 Amino Acid Sequencesmentioning

confidence: 99%

“…The number of coded amino acids for p10 gene could vary among different isolates of nucleopolyhedrovirus and in different species of Lepidoptera. There are some isolates that show 70 amino acid residues, whereas others show 94 ( Figure 2; Hong et al, 2000). 3) and of ORF14 (BmORF14-1, lanes 4 to 6) were done.…”

Section: Bmmnpv Orf10 and Orf14 Amino Acid Sequencesmentioning

confidence: 99%

Conserved baculoviral ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus

Gigliolli

Silva²,

Balani³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) were amplified, cloned and sequenced. Nucleotide analysis of these genes and those of other baculoviruses showed that these genes are highly conserved. The p10 protein from BmMNPV ORF10 has 70 amino acid residues similar to that of the four other known BmNPV strains. The BmMNPV ORF14 alignment showed a higher identity with the nucleopolyhedrovirus ORF14 from the baculovirus BmNPV and from Autographa californica multiple nucleopolyhedrovirus. The BmMNPV ORF14 protein has a putative transmembrane domain in the C-terminal region, which is similar to that of other baculoviruses. A phylogenetic analysis showed that BmMNPV ORF14 protein has higher similarity with BmNPV ORF14 and ORF23 of A. californica multicapsid nucleopolyhedrovirus (Ac23). We conclude that proteins produced by ORFs 10 and 14 from BmNPV and BmMNPV are highly conserved in NPVs and MNPVs. The high degree of conservation among members of these genera indicates the importance of these proteins, which could mean an important function that is active throughout the infection cycle.

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Displaying enhancing factors on the surface of occlusion bodies improves the insecticidal efficacy of a baculovirus

Lei

Yang

Lei

et al. 2019

Pest Management Science

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BACKGROUND Baculoviruses provide long‐lasting control of crop pests and are harmless to humans and non‐target animals, making them attractive bioinsecticides. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) has a wide‐host range and is one such commercial bioinsecticide, but its low infectivity to older larvae and less‐sensitive species precludes its large‐scale application. We sought to improve the infectivity of AcMNPV. RESULTS Two enhancing factors, the truncated enhancin from Agrotis segetum granulovirus and GP37 from Cydia pomonella granulovirus, were expressed in fusion with the N‐terminal and middle domain of the polyhedrin envelope protein of AcMNPV. Western blotting and immunoelectron microscopy analysis indicated that the enhancing factors were expressed on the occlusion bodies of the resulting AcMNPV variants. Bioassays showed that the median lethal doses of the recombinant viruses were 3.9‐fold to 7.4‐fold lower than those of the wild‐type virus against the second and fourth instar of Spodoptera exigua larvae. The yields of occlusion bodies from the two recombinants in S. exigua larvae were comparable with those of the wild‐type virus both in vitro and in vivo. Further bioassays showed that the AcMNPV variants fusing the enhancing factors were incapable of infecting the second instar larvae of S. litura, Helicoverpa armigera, and Pyrausta nubilalis, which were not sensitive to the wild‐type AcMNPV. CONCLUSION These genetically modified AcMNPV variants exhibited an enhanced infectivity and may offer better baculovirus control of crop pests. © 2019 Society of Chemical Industry

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Characterization of four isolates of Bombyx mori nucleopolyhedrovirus

Cited by 27 publications

References 20 publications

Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Conserved baculoviral ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus

Displaying enhancing factors on the surface of occlusion bodies improves the insecticidal efficacy of a baculovirus

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