Characterization of frog muscle mitochondria

Skoog, CM; Kromer, U.; Mitchell, Richard R.; Hoogstraten, Jan; Stephens, N. L.

doi:10.1152/ajpcell.1978.234.1.c1

Cited by 36 publications

(3 citation statements)

References 12 publications

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“…A fundamental assumption made in this study is that the P: 02 in the intact frog sartorius matches that determined by classical in vitro work. Evidence consistent with this proposition has been reported by Skoog et al (1978), who found a P:O ratio of 2.8 ---0.1 in frog skeletal muscle mitochondria at 28~ The results reported here are also consistent with this assumption, but in the present context, A[CP]0/A[O2] cannot properly be considered an estimate of the P: O2 ratio. As stated in Eq.…”

Section: In Vivo P: 02 Ratiosupporting

confidence: 93%

The relationship between initial creatine phosphate breakdown and recovery oxygen consumption for a single isometric tetanus of the frog sartorius muscle at 20 degrees C.

Mahler¹

1979

The Journal of General Physiology

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Section: In Vivo P: 02 Ratiosupporting

confidence: 93%

The relationship between initial creatine phosphate breakdown and recovery oxygen consumption for a single isometric tetanus of the frog sartorius muscle at 20 degrees C.

Mahler¹

1979

The Journal of General Physiology

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“…$ Substrate oxidation enthalpy of 477.0 kJ/mol OZ (carbohydrate) . 3.25 and also lower than the value of 2 .8 reported for isolated frog muscle mitochondria (Kushmerick, 1977 ;Skoog et al, 1978) .…”

Section: Comparison Ofhigh-energy Phosphate Breakdown During Contraction With Steadycontrasting

confidence: 60%

Physical and biochemical energy balance during an isometric tetanus and steady state recovery in frog sartorius at 0 degree C.

Rutgeerts¹

1983

The Journal of General Physiology

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Frog sartorius muscle stimulated isometrically for 3 s every 256 s to attain a steady state in which initial heat ((b), recovery heat (R), rate Of 02 consumption (Jo), and isometric force (P.) generated are constant for each cycle. For a 3-s tetanus given every 256 s, ,Joz was 0.106,umol/(min " g blotted weight),~71% of the maximum rate observed, whereas lactate production was negligible under these conditions . 0, QT( = 0 + QR), and QT/0 were 88 .2, 181 .5, 2.06 mJ/g blotted weight, respectively. The high-energy phosphate breakdown (A-P) breakdown during the first 3-s tetanus was not different from that during a contraction in the steady state and averaged 1.1 gmol/g blotted weight . Less than half of the initial heat could be accounted for in terms of the extent of the known chemical reactions occurring during contraction . From the stoichiometry of the theoretical biochemical pathways, the amount of ATP synthesized in the steady state exceeds 0^"P during contraction by more than twofold, corresponding to an apparent ADP : O ratio of 1 .5 . If it is assumed that carbohydrate oxidation is the only net chemical reaction in the steady state, the total heat production can be explained on the basis of the measured foe . Under this assumption, heat production during recovery was less than that expected on the basis of the oxygen consumption and 0^"P during contraction . These observations support the hypothesis that the unexplained enthalpy production and low apparent ADP :O ratio are causally related, i.e ., that the reaction(s) producing the unexplained heat during contraction is reversed during the recovery period .

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“…Red muscle and heart mitochondria from nonmammalian vertebrates typically oxidize LCFAcarnitine as a preferred substrate, e.g., pigeon breast muscle (Tyler and Gonze 1967;Ernster and Nordenbrand 1967), hummingbird flight muscle (Suarez et al 1986), frog sartorius (Skoog et al 1978), and carp red muscle (Moyes et al 1989). Mammalian skeletal and cardiac muscle mitochondria have little capacity to oxidize LCFA by the carnitine-independent pathway (Bode and Klingenberg 1965;Huxtable and Wakil 197 1).…”

Section: Comparative Aspects Of F a Oxidationmentioning

confidence: 99%

A comparison of fuel preferences of mitochondria from vertebrates and invertebrates

Moyes¹,

Suarez²,

Hochachka³

et al. 1990

Can. J. Zool.

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1990. A comparison of fuel preferences of mitochondria from vertebrates and invertebrates. Can. J. Zool. 68: 1337-1349.Knowledge of tissue-specific mitochondrial properties is important in understanding cellular aerobic energy metabolism. Studies employing isolated mitochondria offer the advantage of direct and controlled manipulation of extramitochondrial conditions, while minimizing disruption of interactions between mitochondrial enzymes, transporters, and membranes. In this review, we compare the oxidative properties of mitochondria isolated from liver, heart, and skeletal muscle of vertebrates and invertebrates. The observed differences between tissues and species in the capacities for mitochondrial oxidation of fatty acids, ketone bodies, pyruvate, and amino acids reflect fundamentally different adaptations for the assimilation, storage, and utilization of metabolic fuels. MOYES, C. D., SUAREZ, R. K., HOCHACHKA, P. W., et BALLANTYNE, J. S. 1990. A comparison of fuel preferences of mitochondria from vertebrates and invertebrates. Can. J. Zool. 68 : 1337-1349. La connaissance des propriCtCs mitochondriales spCcifiques A certains tissus est essentielle A la comprChension du mCtabolisme CnergCtique aCrobie des cellules. Les travaux sur des mitochondries isolCes offrent l'avantage d'une manipulation directe et contr61Ce des conditions extramitochondriales tout en affectant le moins possible les interactions entre les enzymes, les ClCments de transport et les membranes des mitochondries. Dans cette synthkse, nous comparons les propriCtCs oxydatives de mitochondries isolCes du foie, du coeur et du muscle squelettjque de vertCbrCs et d'invertCbrCs. Les diffkrences observCes entre divers tissus et entre diffkrentes especes quant A la capacitC mitochondriale d'oxydation des acides gras, des corps cktoniques, des pyruvates et des acides aminCs sont le reflet des adaptations fondamentalement diffkrentes qui rCgissent l'assimilation, le stockage et l'utilisation des comburants mCtaboliques. [Traduit par la revue]Introduction may provide models that are better suited for the study of Cellular energy production via the oxidation of glucose, fatty acids, ketone bodies, and amino acids requires participation of rnitachondrial transporters, rnitochondrial matrix and membranebound enzymes, and oxidative phosphorylation. Consequently, studies of metabolite oxidation by isolated mitochondria provide insights into the fuel preferences of various tissues. Because studies that make use of isolated mitochondria minimize disruption of enzyme-enzyme, enzyme-membrane, and enzyme-transporter interactions, they are useful in assessing transport capacities, metabolic flux rates, enzyme activities in situ, and regulation of mitochondrial pathways. Such studies may also yield important information about anabolic capacities,

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Characterization of frog muscle mitochondria

Cited by 36 publications

References 12 publications

The relationship between initial creatine phosphate breakdown and recovery oxygen consumption for a single isometric tetanus of the frog sartorius muscle at 20 degrees C.

The relationship between initial creatine phosphate breakdown and recovery oxygen consumption for a single isometric tetanus of the frog sartorius muscle at 20 degrees C.

Physical and biochemical energy balance during an isometric tetanus and steady state recovery in frog sartorius at 0 degree C.

A comparison of fuel preferences of mitochondria from vertebrates and invertebrates

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