Characterization of haemocytes of the Indian edible oyster, Crassostrea madrasensis (Preston)

Abstract

The haemocytes of the Indian edible oyster Crassostrea madrasensis were characterized using light and electron microscopy. The light microscopic study was conducted by staining a monolayer of the haemocytes with Geimsa. Cells without granules and with a large nucleus occupying much of the cytoplasmic area were grouped as hyalinocytes. Those with lesser amounts of basophilic cytoplasmic granules were characterized as semigranulocytes and those with large amounts of a mixture of acidophilic and basophilic granules were termed as granulocytes. Ultrastructural studies also revealed the presence of three types of haemocytes. Scanning electron microscopic studies were used to study the spreading behaviour of the haemocytes. Cytochemical studies revealed the presence of acidphosphatase, peroxidase and prophenol oxidase in the cells.

Characterization of subpopulations and immune-related parameters of hemocytes in the green-lipped mussel Perna viridis

“…When spread on a slide, granulocytes encompassed a relatively large area. Granulocytes could be further divided into three subpopulations (i.e., dense-granulocyte, semigranulocyte and small semi-granulocyte) according to granularity and size of hemocyte [28], which were confirmed by TEM and SEM. Other authors classified granulocytes into two types according to the size of granules, such as small granulocytes and large granulocytes in Argopecten irradians [8] and Cristaria plicata [29].…”

Changes in juvenile sturgeon erythrocytes across age classes reflects evolutionary development

“…The Israeli Journal of Aquaculture -Bamidgeh • IJA.73.2021.1237639 madrasensis) (Ittoop et al, 2006), whereas amphibians (e.g., Hoplobatrachus chinensis and Lithobates catesbeiana) and reptiles (e.g., Trionyx sinensis) have evolved erythrocytes which have surface protrusions and shallow caves (Qing et al, 2011). Mammals' mature erythrocytes have smooth membranes and an oval morphology to facilitate movement in the capillaries (Elgsaeter A and Mikkelsen A.…”

Lesions of Copper Toxicosis in Captive Marine Invertebrates With Comparisons to Normal Histology

“…4 Gills are a commonly injured organ in gastropods with copper toxicosis, and branchial epithelial necrosis is among the most commonly described lesion of this disease; the specificity of these lesions, however, has not been investigated in the referenced studies. 4,13,18,22,32 Injury to the branchial system presumably results in a reduction in oxygen consumption and osmoregulatory capacity. 18,32 Another lesion observed in the California sea hare presented here was axonal degeneration, attributed to a combination of elevated pressure due to osmoregulation dysfunction and direct necrotizing affect to neural cells, the latter of which may be due to cellular metabolic derangements induced by copper toxicosis.…”

“…4,13,18,22,32 Injury to the branchial system presumably results in a reduction in oxygen consumption and osmoregulatory capacity. 18,32 Another lesion observed in the California sea hare presented here was axonal degeneration, attributed to a combination of elevated pressure due to osmoregulation dysfunction and direct necrotizing affect to neural cells, the latter of which may be due to cellular metabolic derangements induced by copper toxicosis. 5 Defects of osmoregulation may have contributed to interstitial edema within the heart and pharyngeal muscle in this case, although a contribution from an inflammatory response, evidenced by increased numbers of circulating hemocytes, was also considered possible.…”