Characterization of herpes simplex virus strains isolated from patients with various diseases

Abstract

Herpes simplex virus strains (HSV) were isolated from various herpetic diseases. These HSV-strains isolated in Bulgaria (687) and in other countries (13) were studied by the neutralization test using standard type specific rabbit antisera and human gamma globulin. The serotype distribution of all strains showed: HSV-1 = 323 strains, HSV-2 = 372 strains, 5 strains behaved antigenically intermediate. A close correlation between the serotype of the strains and the localization of the lesions was established. All but four strains (1.24 per cent) isolated from the head belong to HSV-1, and only six HSV-strains (1.9 per cent) from lesions with other localizations were not HSV-2. Within six months to 8 years 2 to 4 herpes strains were isolated repeatedly from 30 patients. In 23 of these cases they were identical and in the other they showed different biological (3) or antigenical (4) properties. The results suggest, that under natural conditions some HSV-intermediate strains may exist.

A Comparative Analysis of Restriction Enzyme Digests of the DNA of Herpes Simplex Virus Isolated from Genital and Facial Lesions

“…For example, HSV-1 is the predominant serotype associated with common recurring facial lesions and in rare instances herpes encephalitis, whereas HSV-2 is most frequently associated with recurrent infection of the genital regions of adults and less frequently with generalized infection of neonates (Dundarov et al, 1980;Terni & Roizman, 1970). A considerable body of evidence based both on serology and on molecular hybridization now suggests close association of HSV-2 with carcinoma of the cervix (Rawls et al, 1977;Dowdle et al, 1967;Naib et al, 1969;McDougall et al, 1980;Eglin et al, 1981).…”

Could Probiotics and Postbiotics Function as “Silver Bullet” in the Post-COVID-19 Era?

“…In other studies, Serkedjieva et al [41] reported that a Lactobacillus delbrueckii bacteriocin had virus-inhibitory activity. Previously, Dundarov and Andonov [42] had demonstrated a strong anti-herpesvirus effect of the ionophore antibiotic pandavir (nigericin). Pandavir inhibited virus reproduction, even at 0.01-0.02 ng/mL, by specific inhibition of virus DNA synthesis.…”

Developments in herpes simplex virus vaccines: Old problems and new challenges

“…The earliest efforts to develop an anti-herpes vaccine started over 60 years ago. The use of a crude protein extract from HSV-1-and HSV-2-infected cells, which had been inactivated by chemical means, was reported to be beneficial by several authors (Kern and Schiff 1964;Skinner et al 1980;Dundarov et al 1982;Kavaklova et al 1986). The early vaccines (such as the Dundarov vaccine) were not tested for the presence of residual cellular components, which may contaminate the antigen product.…”

“…The Bulgarian (Dundarov) vaccine belonged to the category of inactivated but poorly purified infected cell extracts containing a mix of 5 HSV-1 and HSV-2 strains. The first report registered over 2300 vaccinated persons in an open noncontrolled trial (Dundarov et al 1982). Later on during the 1980s over 5000 patients were vaccinated (S. Dundarov, personal communication).…”