Characterization of in Vitro Modified Human Very Low-Density Lipoprotein Particles and Phospholipids by Capillary Electrophoresis

Liu, Yining; Shu, Ting-Yu; Xie, Huai-Guang; Lai, Wei-Ting; Liao, Yi Han; Su, Mei-Yu; Lin, You-Sian; Chen, Yen-Yi; Lin, Yi-Chun; Chong, Chin-Pong; Liu, Mine-Yine

doi:10.3390/ijms131216400

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Liu et al. described the analysis of in vitro oxidized and glycated human very low density lipoprotein in human plasma by MEKC with high‐salt stacking. The BGS contained 10 mM phosphate buffer and 50 mM bile salts (50% sodium cholate and 50% sodium deoxycholate) at pH 8.5 in 30% 1‐propanol and the sample buffer was 100 mM NaCl in 20% 1‐propanol.…”

Section: Micellar Methodsmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2014

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This contribution is a methodological review of the publications about the topic from the last 2 years. Therefore, it is primarily organized according to the methods and procedures used in surveyed papers and the origin and type of sample and specification of analytes form the secondary structure. The introductory part about navigation in the architecture of stacking brings a brief characterization of the various stacking methods, with the description of mutual links to each other and important differences among them. The main body of the article brings a survey of publications organized according to main principles of stacking and then according to the origin and type of the sample. Provided that the paper cited gave explicitly the relevant data, information about the BGE(s) used, procedure, detector employed, and reached LOD and/or concentration effect is given. The papers where the procedure used is a combination of diverse fragments and parts of various stacking techniques are mentioned in a special section on combined techniques. The concluding remarks in the final part of the review evaluate present state of art and the trends of sample stacking in CE.

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Section: Micellar Methodsmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2014

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“…Recently, we have also reported a study for the analysis of in vitro oxidized human VLDL phospholipids by SPE and MEKC methods. 8 However, the MEKC conditions used in this study were different from the previous VLDL study. The MEKC analysis of the total lipids and the four SPE lipid fractions of VLDL are first reported in this study.…”

Section: Introductionmentioning

confidence: 93%

Separation of Lipids on Human Very Low‐density Lipoproteins by Micellar Electrokinetic Chromatography

Wei¹,

Liao²,

Xie³

et al. 2013

J Chinese Chemical Soc

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Liquid-phase and solid-phase extractions (SPE) in combination with a simple micellar electrokinetic chromatography (MEKC) method were used to investigate human very low-density lipoprotein (VLDL) lipids for two healthy donors. At absorbance 200 nm, the effective mobilities and peak areas of the MEKC profiles showed good reproducibility and precisions. A major peak and several minor peaks appeared for the total lipids of native VLDL, but both the peak numbers and areas reduced for the in vitro oxidized VLDL. Two chloroform and two methanol fractions were obtained from SPE of VLDL total lipids. Significant differences were observed for the first methanol fraction between native and in vitro oxidized VLDL lipids. The first methanol fraction showed a major peak and several minor peaks for native VLDL, but both the peak numbers and areas reduced for oxidized VLDL. Oxidation of VLDL caused decomposition of lipids, and thus the reduction of peak numbers and areas.

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Recent advances in capillary electrophoresis separation of monosaccharides, oligosaccharides, and polysaccharides

et al. 2017

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Recent advances in capillary electrophoresis separation of monosaccharides, oligosaccharides, and polysaccharidesThis article illustrates the basis and applications of methodologies for the analysis of simple and complex carbohydrates by means of CE. After a description of the most common and novel approaches useful for the analysis and characterization of carbohydrates, this review covers the recent advances in CE separation of monosaccharides, oligosaccharides, and polysaccharides. Various CE techniques are also illustrated for the study of carbohydrates derived from complex glyco-derivatives such as glycoproteins and glycolipids, essential for biopharmaceutical and glycoproteomics applications as well as for biomarker detection. Most glycans have no significant UV absorption, and derivatization with fluorophore groups prior to separation usually results in higher sensitivity and an improved electrophoretic profile. We also discuss the recent applications and separations by CE of derivatized simple and more complex carbohydrates with different chromophoric active tags. Overall, this review aims to give an overview of the most recent state-of-the-art techniques used in carbohydrate analysis by CE. Keywords:Capillary Electrophoresis / Laser-induced fluorescence / Monosaccharides / Oligosaccharides / Polysaccharides DOI 10.1002/elps.201700290 IntroductionCarbohydrates are considered important key molecules and the largest group of biomolecules. Among these, monosaccharides are the simplest units forming complex macromolecules and glucose is certainly one of the most important carbohydrates for life. Furthermore, complex macromolecules include diverse subgroups such as polysaccharides, proteoglycans, glycolipids, and glycoproteins. Carbohydrates are also a relatively new subject of medical research as they play important roles in biological functions such as cell-cell and cell-host interaction, regulation of the pro-or anti-inflammatory abilities of immunoglobulins according to their level of sialylation, cellular trafficking, solubility of proteins, and assistance in protein folding. Alterations Qualitative and quantitative elucidation of carbohydrates is a challenging task due to the lack of either chromophoric or fluorophoric functional groups and low extinction coefficients of the monosaccharide structure. Moreover, the structural complexities of glycans, along with their diversity [5], and the unique tendency toward branching, different linkages, and isomerism generally present formidable challenges.CE has attracted considerable attention in the recent advances in glycomic analysis. CE is suitable for both derivatized and underivatized carbohydrates and it is established as a powerful and versatile separation tool because of the brief analysis time, the ability to handle very complex glycan mixtures with high sensitivity, the minute quantities of samples and reagent required, the capacity to resolve isomeric glycans, and the possibility to acquire high resolution obtained under physiological conditions [5]. I...

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Characterization of in Vitro Modified Human Very Low-Density Lipoprotein Particles and Phospholipids by Capillary Electrophoresis

Cited by 3 publications

References 33 publications

Contemporary sample stacking in analytical electrophoresis

Contemporary sample stacking in analytical electrophoresis

Separation of Lipids on Human Very Low‐density Lipoproteins by Micellar Electrokinetic Chromatography

Recent advances in capillary electrophoresis separation of monosaccharides, oligosaccharides, and polysaccharides

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