Separation of Lipids on Human Very Low‐density Lipoproteins by Micellar Electrokinetic Chromatography

Wei, Lai; Liao, Yi Han; Xie, Huai Guang; Liu, Yi Ning; Lin, Yi Jyun; Liu, Mine-Yine

doi:10.1002/jccs.201300103

Cited by 2 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…),and Large subsidiary structure (cross-channel contact, inclined shafts, underground substations, wind the engine room, etc. );(3) Complicated geological conditions: as the extra-shallow buried, karst, fault fracture zone,and rock burst;(4) The section spacing laid covering different depths; (5) The monitoring section layout around staggered.After comprehensive consideration, 29 typical sections are selected to be monitored. These 29 sections represent the tunnel crossing at different depths, different surrounding rock, the different types of section lining the main tunnel and subsidiary structure and the complex geology of the various conditions.…”

Section: Scheme Of Monitoring and Measurementmentioning

confidence: 99%

See 1 more Smart Citation

Field Monitoring and Numerical Simulation Study on Mechanical Characteristics of Extra-Long Highway Tunnel

2012

AMM

View full text Add to dashboard Cite

Taking Motianling tunnel in Chongqing as an engineering background, the monitoring and measurement scheme is proposed for the extra-long tunnel under complex condition, and the field data and the numerical simulation results is analyzed. The results indicates that, the selected cross-section reflect overall state of the Motianling tunnel, and the structure safety monitoring during operation is considered; by tunnel internal and external observation, it is found that the tunnel face in F22 fault zone may not stay stability, because the monitoring information receive feedback timely, and the supporting measures is proper, the surrounding rock stay stability when excavating; for the typical section, the surrounding rock contact pressure is greater than interlayer pressure, primary lining sustains most of the load ,and the secondary lining plays an assistant role as safety reservation; the maximum of anchor axial force and surrounding rock pressure occur at tunnel crown. By monitoring and numerical simulation, the dynamic excavation and design is realized, the Motianling tunnel is achieved safely and successfully at last.

show abstract

Section: Scheme Of Monitoring and Measurementmentioning

confidence: 99%

“…Supporting Structure. Support structure stress condition is judged mainly by the surrounding rock contact pressure, interlayer pressure, and axial force [5]. The ZK46+370 will serve to illustrate it.…”

Section: Mechanical Characteristics Of Tunnelmentioning

confidence: 99%

Field Monitoring and Numerical Simulation Study on Mechanical Characteristics of Extra-Long Highway Tunnel

2012

AMM

View full text Add to dashboard Cite

show abstract

Separation of total lipids on human lipoproteins using surfactant‐coated multiwalled carbon nanotubes as pseudostationary phase in capillary electrophoresis

Chen

Yang

et al. 2013

Electrophoresis

View full text Add to dashboard Cite

Surfactant-coated multiwalled carbon nanotubes (MWNTs) were used as pseudostationary phase (PSP) in CE to investigate the total lipids of high-density lipoproteins and low-density lipoproteins. To optimize the CE conditions, several experimental factors including carbon nanotube concentration, bile salt concentration, sodium phosphate (PB) concentration, organic modifier concentration and buffer pH value have been examined. In addition, the CE capillary temperature and applied voltage have also been examined. The optimal separation buffer selected was a mixture of 3.2 mg/L MWNT, 50 mM bile salt, 10 mM PB, 20% 1-propanol, pH 9.5. The optimal capillary temperature and applied voltage selected were 50°C and 20 kV, respectively. Phosphatidyl choline (PC) has been used as a model analyte and investigated by the optimal CE method. The linear range for PC was 0.1-3 mg/mL with a correlation coefficient of 0.9934, and the concentration LOD was 0.055 mg/mL. The optimal CE method has been used to characterize the total lipids of high-density lipoprotein and low-density lipoprotein. At absorbance 200 nm, one major peak and two or three minor peaks showed for the total lipids of lipoproteins within 13 minutes. Resolutions of the total lipids were enhanced using surfactant-coated MWNTs as PSPs in the CE separation buffer. However, resolutions of the total lipids were not enhanced using surfactant-coated single-walled carbon nanotubes as PSPs in the CE separation buffer.

show abstract

Separation of Lipids on Human Very Low‐density Lipoproteins by Micellar Electrokinetic Chromatography

Cited by 2 publications

References 9 publications

Field Monitoring and Numerical Simulation Study on Mechanical Characteristics of Extra-Long Highway Tunnel

Field Monitoring and Numerical Simulation Study on Mechanical Characteristics of Extra-Long Highway Tunnel

Separation of total lipids on human lipoproteins using surfactant‐coated multiwalled carbon nanotubes as pseudostationary phase in capillary electrophoresis

Contact Info

Product

Resources

About