Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis

Abstract

Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93.4% of the strains. Of which, TEM was the most prevalent (93.4%), followed by CTX-M and SHV were 57.6% and 39.4%, respectively. A main mutation pattern of quinoloneresistance-determining region, Thr83-Ieu and Asp87-Asn in gyrA and Ser87-Ile in parC, was detected in 33 K. pneumoniae isolates. All the isolates harbored at least two virulence factor genes, with ureA (97.0%) and wabG (91.0%) exhibiting high carriage rates in 33 K. pneumoniae isolates. MLST revealed 7 sequence types, of which 3 STs (2541, 2581 and 2844) were newly assigned. Using eBURST, ST2844 and ST2541 were assigned to new clonal complex 2844. Our study provides evidence and biological characteristics of K. pneumoniae isolates from cattle upper respiratory tract in Southwest China.

First evidence of Klebsiella pneumoniae infection in Aceh cattle: Pathomorphology and antigenic distribution in the lungs

“…Klebsiella pneumoniae is a facultative anaerobic, Gram-negative, rod-shaped, non-motile encapsulated bacterium capable of fermenting glucose and lactose, which can cause infection in humans, animals, and plants [ 1 , 2 ]. K. pneumoniae were classified into classical K. pneumoniae and hypervirulent K. pneumonia (hvKp) strains based on their genotypic and phenotypic biomarkers [ 3 ].…”

“…K. pneumoniae is considered one of the most important pathogens in public health because they are animal-borne bacterial pathogens (zoonoses) [ 1 ]. In humans, K. pneumoniae has been associated with pneumonia, urinary tract infections, bacteremia, and liver abscess, primarily in immunocompromised hosts [ 11 , 12 ].…”

“…To the best of our knowledge, one study published the isolation of KP in only one pneumonic lung of Bali cattle in Indonesia [ 21 ], but the capsular serotypes, histopathology, and distribution of the bacteria are not available in the literature. Recently, 12 isolates belonging to hvKp have been detected from cattle nasal swabs in Southwest China [ 1 ]. However, the information about the capsular serotypes of K. pneumoniae in cattle is still limited, and the pathological evidence has been poorly investigated.…”

Detection of Multidrug Resistant Gram Negative Bacteria in Healthy Cattle from Maiduguri Metropolitan, Nigeria

“…In the present study, MDR Klebsiella spp was found to be 80% (Table 1). In a study from China, high rate resistance Klebsiella spp was reported to be 93.4% [45]. Similar studies documented MDR Klebsiella spp in cattle [46,47,48,49].…”

Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods

“…K. pneumonia contamination was recorded in 32% of the street food samples examined in Malaysia [8]. This bacterium is also an issue of domestic animals that threatens livestock production as well as scourging public health since these animals could serve as a vehicle for multidrug-resistant K. pneumoniae strains [9].…”