Characterization of Lipid-Based Hexosomes as Versatile Vaccine Carriers

Rodrigues, Letícia G.; Kyriakos, Konstantinos; Schneider, Fabian; Dietz, Hendrik; Winter, Gerhard; Papadakis, Christine M.; Hubert, Madlen

doi:10.1021/acs.molpharmaceut.6b00716

Cited by 36 publications

(19 citation statements)

References 42 publications

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“…In the presence of a proper stabilizer [ 6 ], liquid crystal phases can be arranged in different supramolecular structures such as cubosomes [ 7 , 8 ] or hexosomes [ 9 , 10 , 11 ], which could potentially be used for intravenous injection. This is because of their scarce viscosity as well as their ability to maintain the internal nanostructure of the bulk systems and keep it intact.…”

Section: Introductionmentioning

confidence: 99%

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Gagliardi

Cosco

Udongo³

et al. 2020

Pharmaceutics

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Glyceryl monooleate (GMO) is one of the most popular amphiphilic lipids, which, in the presence of different amounts of water and a proper amount of stabilizer, can promote the development of well defined, thermodynamically stable nanostructures, called lyotropic liquid crystal dispersions. The aim of this study is based on the design, characterization, and evaluation of the cytotoxicity of lyotropic liquid crystal nanostructures containing a model anticancer drug such as doxorubicin hydrochloride. The drug is efficiently retained by the GMO nanosystems by a remote loading approach. The nanostructures prepared with different non-ionic surfactants (poloxamers and polysorbates) are characterized by different physico-chemical features as a function of several parameters, i.e., serum stability, temperature, and different pH values, as well as the amount of cryoprotectants used to obtain suitable freeze-dried systems. The nanostructures prepared with poloxamer 407 used as a stabilizer show an increased toxicity of the entrapped drug on breast cancer cell lines (MCF-7 and MDA-MB-231) due to their ability to sensitize multidrug-resistant (MDR) tumor cells through the inhibition of specific drug efflux transporters. Moreover, the interaction between the nanostructures and the cells occurs after just a few hours, evidencing a huge cellular uptake of the nanosystems.

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Section: Introductionmentioning

confidence: 99%

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Gagliardi

Cosco

Udongo³

et al. 2020

Pharmaceutics

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“…21 − 57 Among the nanostructured carriers, the liquid crystalline lipid assemblies have shown considerable potential in protecting instable encapsulated molecules from degradation and enhancing their bioavailability and diffusion through the biological barriers. 2 , 15 , 24 , 36 , 43 , 47 − 50 …”

Section: Introductionmentioning

confidence: 99%

“…Such carrier particles (cubosomes, hexosomes, or spongosomes) do not rapidly break upon contact with biological fluids after administration and may provide sustained release. 3 , 6 , 24 , 43 , 44 , 49 Moreover, they can be sterically stabilized by hydrophilic shells to avoid eventual aggregation in the milieu. 3 , 10 , 14 , 29 , 47 , 48 …”

Section: Introductionmentioning

confidence: 99%

Liquid Crystalline Nanostructures as PEGylated Reservoirs of Omega-3 Polyunsaturated Fatty Acids: Structural Insights toward Delivery Formulations against Neurodegenerative Disorders

et al. 2018

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Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are bioactive lipids with considerable impact in medicine and nutrition. These compounds exert structuring effects on the cellular membrane organization, regulate the gene expression, and modulate various signaling cascades and metabolic processes. The purpose of the present work is to demonstrate the structural features of ω-3 PUFA-containing three-dimensional supramolecular lipid assemblies suitable for pharmaceutical applications that require soft porous carriers. We investigate the liquid crystalline structures formed upon mixing of eicosapentaenoic acid (EPA, 20:5) with the lyotropic nonlamellar lipid monoolein and the formation of multicompartment assemblies. Starting with the monoolein-based lipid cubic phase, double membrane vesicles, cubosome precursors, sponge-type particles (spongosomes), mixed intermediate nonlamellar structures, and multicompartment assemblies are obtained through self-assembly at different amphiphilic compositions. The dispersions containing spongosomes as well as nanocarriers with oil and vesicular compartments are stabilized by PEGylation of the lipid/water interfaces using a phospholipid with a poly(ethylene glycol) chain. The microstructures of the bulk mixtures were examined by cross-polarized light optical microscopy. The dispersed liquid crystalline structures and intermediate states were studied by small-angle X-ray scattering, cryogenic transmission electron microscopy, and quasielastic light scattering techniques. They established that PUFA influences the phase type and the sizes of the aqueous compartments of the liquid crystalline carriers. The resulting multicompartment systems and stealth nanosponges may serve as mesoporous reservoirs for coencapsulation of ω-3 PUFA (e.g., EPA) with water-insoluble drugs and hydrophilic macromolecules toward development of combination treatment strategies of neurodegenerative and other diseases.

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“…In excess of water, LLC can be dispersed into sub-micron sized colloidal particles, maintaining the internal nanostructure of the bulk system [ 16 , 17 , 18 , 19 ]. Thus, thermodynamically stable colloidal dispersions of the inverse bicontinuous cubic phases or hexagonal phases, named cubosomes or hexosomes, respectively, can be also produced, making appealing drug delivery systems [ 14 , 20 , 21 , 22 , 23 ]. The colloidal stability of these LLC particles, also known as ISAsomes (Internally Self-Assembled particles), is achieved by the action of stabilizing polymers [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Hexosomes with Undecylenic Acid Efficient against Candida albicans

et al. 2018

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Due to the growing issues with fungal infections, especially with Candida, there is still a need to develop novel anti-Candida materials. One of the known antifungal agents is undecylenic acid (UA), which still cannot be efficiently used due to its oily nature, and thus limited solubility. By taking advantage of the properties of UA, we developed an emulsion with hexagonal phase, i.e., hexosomes, whose structure and morphology was studied by small-angle X-ray scattering and cryo-electron microscopy, respectively. The presence of UA in the hexosome was confirmed by spectroscopy. Moreover, we studied the anti-Candida effect of hexosomes and their cytotoxicity toward human cells. The minimal inhibitory concentration for the 50% and 90% Candida-growth reduction was found at 0.01 and 0.16 wt % hexosomes, respectively (i.e., 2 and 32 pghex/C.a.cell, respectively). The percentage of metabolically active Candida was reduced by 72–96% at hexosome concentrations of 1.0–8.2 pghex/C.a.cell as compared to untreated Candida. Furthermore, at the same concentration range the embedded filamentation test after 24 and 48 h showed the inhibition of both the filamentation and growth of Candida, while the preliminary toxicity test showed that hexosomes were nontoxic for human cells. All these render the here-developed hexosomes with UA efficient and promising anti-Candida agents.

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Characterization of Lipid-Based Hexosomes as Versatile Vaccine Carriers

Cited by 36 publications

References 42 publications

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Liquid Crystalline Nanostructures as PEGylated Reservoirs of Omega-3 Polyunsaturated Fatty Acids: Structural Insights toward Delivery Formulations against Neurodegenerative Disorders

Hexosomes with Undecylenic Acid Efficient against Candida albicans

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