Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates

García-Álvarez, Andrés; Vela, Ana Isabel; Martín, E. San; Cháves, Fernando; Fernández‐Garayzábal, J. F.; Domı́nguez, Lucas; Hierro, Eva

doi:10.1016/j.vetmic.2017.04.015

Cited by 35 publications

(18 citation statements)

References 29 publications

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“…When we used the Multiple host MLST database to re-assigned these strains, we found that all ST50, ST13, and ST74 strains were re-assigned as ST11, ST3, and ST10, respectively. These results are in agreement with the latest epidemical data from China (Peng et al, 2018 ), and also in agreement with the data from Spain (García-Alvarez et al, 2017 ).…”

Section: Discussionsupporting

confidence: 93%

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

et al. 2018

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Pasteurella multocida is a leading cause of respiratory diseases in many host species. To understand the genetic characteristics of P. multocida strains isolated from different host species, we sequenced the genomic DNA of P. multocida isolated from pigs and analyzed the genetic characteristics of strains from avian species, bovine species, pigs, and rabbits using whole genome sequence (WGS) data. Our results found that a capsular: lipopolysaccharide (LPS): multilocus sequence typing (MLST) genotype A: L1: ST129 (43.75%) was predominant in avian P. multocida; while genotypes B: L2: ST122 (60.00%) and A: L3: ST79 (30.00%) were predominate in bovine P. multocida; genotype D: L6: ST50 (37.50%) in porcine P. multocida; and genotype A: L3: ST9 (76.47%) in rabbit P. multocida. Comparative genomic analysis of P. multocida from different host species found that there are no genes in the P. multocida genome that are specific to any type of host. Phylogenetic analysis using either whole-genome single nucleotide polymorphisms (SNPs) or the set of SNPs present in all single-copy core genes across genomes showed that P. multocida strains with the same LPS genotype and MLST genotype were clustered together, suggesting the combining both the LPS and MLST typing schemes better explained the topology seen in the P. multocida phylogeny.

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Section: Discussionsupporting

confidence: 93%

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

et al. 2018

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“…P. multocida is genetically diverse on the basis of numerous studies [ 5 , 13 , 24 ]. Since different subspecies, serogroups and molecular characterization of P. multocida have different epidemiological significances in the fowl cholera disease, in order to effectively prevent this disease, it is important to identify the biological characteristics of P. multocida clinical isolates that causes infections.…”

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confidence: 99%

“…Gene technologies such as 16S rRNA , restriction endonuclease analysis (REA), ribotyping, random amplification of polymorphic DNA (RAPD)-PCR, pulsed-field gel electrophoresis (PFGE) and multilocus sequence type analysis (MLST), have pushed forward studies of molecular epidemiology and genetic diversity of P. multocida [ 24 , 35 , 43 ]. MLST is considered the appropriate bacterial typing techniques for global and long-term studies [ 13 , 36 ] and the results can be easily compared among different laboratories for long-term and global surveillance of bacterial strains [ 36 , 43 ]. MLST is effective in identifying cases or outbreaks related to P. multocida infection, as well as identifying the source and dissemination events [ 33 ].…”

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confidence: 99%

Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Cheng

Lan

et al. 2018

The Journal of Veterinary Medical Science

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Fowl cholera caused by Pasteurella multocida has always been a disease of global importance for poultry production. The aim of this study was to obtain more information about the epidemiology of avian P. multocida infection in southwest China and the genetic characteristics of clinical isolates. P. multocida isolates were characterized by biochemical and molecular-biological methods. The distributions of the capsular serogroups, the phenotypic antimicrobial resistance profiles, lipopolysaccharide (LPS) genotyping and the presence of 19 virulence genes were investigated in 45 isolates of P. multocida that were associated with clinical disease in poultry. The genetic diversity of P. multocida strains was performed by 16S rRNA and rpoB gene sequence analysis as well as multilocus sequence typing (MLST). The results showed that most (80.0%) of the P. multocida isolates in this study represented special P. multocida subspecies, and 71.1% of the isolates showed multiple-drug resistance. 45 isolates belonged to capsular types: A (100%) and two LPS genotypes: L1 (95.6%) and L3 (4.4%). MLST revealed two new alleles (pmi77 and gdh57) and one new sequence type (ST342). ST129 types dominated in 45 P. multocida isolates. Isolates belonging to ST129 were with the genes ompH+plpB+ptfA+tonB, whereas ST342 included isolates with fur+hgbA+tonB genes. Population genetic analysis and the MLST results revealed that at least one new ST genotype was present in the avian P. multocida in China. These findings provide novel insights into the epidemiological characteristics of avian P. multocida isolates in southwest China.

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“…In 2004 and 2010, two multilocus sequencing typing systems were also developed to genotype P. multocida strains (https://pubmlst.org/pmultocida/) from multiple mammalian hosts and birds, respectively [16,17]. In 2017, a virulence genotyping system based on the detection of different virulence factor gene (VFG) pro les was also reported for distinguishing P. multocida strains from different hosts [18]. Compared to the traditional serological typing methods, these molecular DNA-based typing systems are indeed highly effective and accurate, and they are now wildly used to determine the epidemiological and genetic characteristics of clinical isolates [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Development of a Rapid, Efficient, Intelligent and Cost-Saving Tool to Diagnose Pasteurella Multocida by Using Whole Genome Sequence and Genotypes of Pasteurella Multocida From Different Hosts

Peng

Liu

Liang

et al. 2020

Preprint

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Background: Different typing systems including capsular genotyping, lipopolysaccharide (LPS) genotyping, multilocus sequence typing (MLST), and virulence genotyping based on the detection of different virulence factor-encoding gene (VFG) profiles have been applied to characterize Pasteurella multocida strains from different host species. However, these methods require much time and effort in laboratories. Particularly, relying on one of these methods is difficult to address the biology of P. multocida from host species. Recently, we found that assigning P. multocida strains according to the combination of their capsular, LPS, and MLST genotypes (marked as capsular genotype: LPS genotype: MLST genotype) could help address the biological characteristics of P. multocida circulation in multiple hosts. However, it is still lack of a rapid, efficient, intelligent and cost-saving tool to diagnose P. multocida according to this system. Results: We have developed an intelligent genotyping and host tropism prediction tool PmGT for P. multocida strains according to their whole genome sequences by using machine learning and web 2.0 technologies. By using this tool, the capsular genotypes, LPS genotypes, and MLST genotypes as well as the main VFGs of P. multocida isolates in different host species were determined based on whole genome sequences. The results revealed a closer association between the genotypes and pasteurellosis rather than between genotypes and host species. Finally, we also used PmGT to predict the host species of P. multocida strains with the same capsular: lipopolysaccharide: MLST genotypes. Conclusions: With the advent of high-quality, inexpensive DNA sequencing, this platform represents a more efficient and cost-saving tool for P. multocida diagnosis in both epidemiological studies and clinical settings.

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Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates

Cited by 35 publications

References 29 publications

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

Investigation of genetic diversity and epidemiological characteristics of <i>Pasteurella multocida</i> isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Development of a Rapid, Efficient, Intelligent and Cost-Saving Tool to Diagnose Pasteurella Multocida by Using Whole Genome Sequence and Genotypes of Pasteurella Multocida From Different Hosts

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