Characterization of Plasmids from Antibiotic-resistant Shigella Isolates by Agarose Gel Electrophoresis

Jamieson, A. F.; Bremner, D. A.; Bergquist, Peter L.; Lane, H. E. D.

doi:10.1099/00221287-113-1-73

Cited by 30 publications

(24 citation statements)

References 7 publications

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“…However, previously published reports revealed a heterogeneous plasmid population in strains of S. flexneri, with most plasmids being smaller than 6 MDa (17,42). A correlation between serotype and plasmid pattern was described in a previous report, in which it was suggested that the plasmid profile might be useful in the identification of epidemic clones of S. flexneri as they are introduced in a population (24).…”

Section: Resultsmentioning

confidence: 84%

Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Talukder

Islam

et al. 2003

J Clin Microbiol

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The serotypes of 144 strains of Shigella flexneri serotype 1 (serotypes 1a, 1b, and 1c) isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease Research, Bangladesh, between 1997 and 2001 were serologically confirmed by using commercially available antisera and a panel of monoclonal antibodies specific for S. flexneri group and type factor antigen (MASF). Among serotype 1 isolates, the prevalence of provisional serotype S. flexneri 1c increased from 0 to 56% from 1978 to 2001 in Bangladesh. Detailed biochemical studies revealed that none of the strains of serotype 1 produced indole, while all the strains fermented mannose, mannitol, and trehalose. Twenty percent of the serotype 1c and all the serotype 1a strains fermented maltose and 53% of the serotype 1c strains and 60% of the serotype 1a strains fermented arabinose, whereas all serotype 1b strains were negative for fermentation of these sugars. Only 18% of serotype 1b strains were resistant to nalidixic acid, and most of the serotype 1c and 1b strains were resistant to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. All the strains of serotypes 1a and 1b and about 88% of the serotype 1c strains were found to be invasive by the Sereny test, had a 140-MDa plasmid, and had Congo red absorption ability. Plasmid profile analysis showed that 26% of the strains of serotype 1 contained identical patterns. Most of the serotype 1c strains (72%) had the 1.6-MDa plasmid, which was not found in either serotype 1a or 1b strains. A self-transmissible middle-range plasmid (35 to 80 MDa) was found in some strains carrying the multiple-antibiotic-resistance gene. Pulsed-field gel electrophoresis analysis yielded three types (types A, B, and C) with numerous subtypes among the serotype 1c strains, whereas serotypes 1b and 1a yielded only one type for each serotype, and those types were related to the types for serotype 1c strains. Ribotyping analysis yielded three patterns for serotype 1c strains and one pattern each for serotype 1a and 1b strains which were similar to the patterns for the serotype 1c strains. Overall analysis of the results concluded that subserotype 1c is closely related to serotypes 1a and 1b. Furthermore, the high rate of prevalence of serotype 1c necessitates the commercial production of antibody against this subserotype to allow the determination of the actual burden of shigellosis caused by provisional serotype 1c.

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Section: Resultsmentioning

confidence: 84%

Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Talukder

Islam

et al. 2003

J Clin Microbiol

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“…Shigellae usually harbour various plasmids, such as those required for bacterial invasion into the host intestinal epithelial cells and antibiotic resistance [17]. As many as 10 plasmids were contained in one strain [18]. Thus, plasmid profile analysis is one of the appropriate methods for molecular characterization of shigellae, while the technique is less useful for the bacteria that have only a few or no plasmids, e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Results conformed with the data reported elsewhere that Shigella spp. harbours heterogeneous population of plasmids [17,18].…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

et al. 2006

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“…The possibility also exists that these genes reside on a mobile genetic element acquired from another source. The finding that a region of DNA homologous to msgA exists on one of several cryptic plasmids known to exist in Shigella species (20) suggests a possible role of extrachromosomal elements in the mode of transfer of this region.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Salmonella typhimurium pagC/pagD chromosomal region

et al. 1995

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The PhoP/PhoQ two-component system regulates Salmonella typhimurium genes that are essential to bacterial virulence and survival within macrophages. The best characterized of these PhoP-activated genes (pag) is pagC, which encodes a 188-amino-acid envelope protein (W. S. Pulkkinen and S. I. Miller, J. Bacteriol. 173:86-93, 1991). We here report the identification of four genes (pagD, envE, msgA, and envF) located 5 to pagC. Each gene is transcribed from its own promoter, two of which (msgA and pagD) were defined by primer extension analysis. Three of these genes (pagD, envE, and envF) are predicted to encode envelope proteins. The pagD gene is transcribed in a direction opposite from that of and adjacent to pagC and is positively regulated by PhoP/PhoQ. Transposon insertions within pagD and msgA attenuate bacterial virulence and survival within macrophages; however, deletion of pagD has no effect on virulence. The product of the envF gene is predicted to be a lipoprotein on the basis of the presence of a consensus lipid attachment site. The low G؉C content of these genes and the homology of msgA to Shigella plasmid DNA suggest that this region may have been acquired by horizontal transmission.

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Characterization of Plasmids from Antibiotic-resistant Shigella Isolates by Agarose Gel Electrophoresis

Cited by 30 publications

References 7 publications

Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh

Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

Characterization of the Salmonella typhimurium pagC/pagD chromosomal region

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