Characterization of powdered turmeric by liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry

Hiserodt, Richard D.; Hartman, Thomas G.; Ho, Chi‐Tang; Rosén, Robert

doi:10.1016/0021-9673(96)00103-3

Cited by 97 publications

(44 citation statements)

References 17 publications

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“…The three major curcuminoids found in turmeric are curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) (Hiserodt et al 1996). These curcuminoids are typically measured in turmeric-based extracts and dietary supplements for product strength.…”

Section: Introductionmentioning

confidence: 99%

Curcuminoids in Turmeric Roots and Supplements: Method Optimization and Validation

et al. 2015

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Curcuma longa L. rhizomes are used extensively as a spice in food preparations and dietary supplements for their anti-inflammatory and antioxidant properties. An expert review panel (ERP) evaluated analytical methods for the quantitation of individual curcuminoids for the purpose of identifying a method for official method status. It was requested that several modifications be undertaken to improve method performance prior to subjecting the chosen method to a singlelaboratory validation. Two separate Plackett-Burman factorial studies were used to identify factors that contributed to the chromatographic separation and extraction of curcuminoids. Significant factors were further optimized to produce the improved HPLC method for curcuminoid separation. This method was then subjected to a single-laboratory validation according to the AOAC International guidelines for linearity, detection limits, precision, and accuracy. The two most significant factors impacting the quantitation of curcuminoids were column temperature and extraction solvent, which were optimized to 55°C and 100 % methanol, respectively. The validation was performed on 12 raw materials and finished products containing turmeric roots. The method precision was reported using HorRat values which were within recommended ranges of the AOAC guidelines. Overall accuracy of the method was accessed at three separate levels for each analyte and ranged from 99.3-100.9 %. The validated method is suitable for quantitation of individual curcuminoids in turmeric raw materials and finished products and is recommended for consideration as an official method by the AOAC International.

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Section: Introductionmentioning

confidence: 99%

Curcuminoids in Turmeric Roots and Supplements: Method Optimization and Validation

et al. 2015

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“…Curcumin has recently attracted increased attention because of s potent anti-leishmanial properties 6 , and ability to reduce alcohol-induced liver disease 7 and Alzheimer's disease 8 . Several methods have been developed to separate the curcuminoids: thin-layer chromatography (TLC) 9 , supercritical fluid chromatography (SFC) 10 , gas chromatography (GC) 11 , high performance liquid chromatography (HPLC) 12,13 , and HPLC-mass spectrometry (LC-MS) 11,14 .…”

Section: Introductionmentioning

confidence: 99%

Potential of Ethanolic Extract of Ficus Benghalensis on Open Wounds and Inflammation

Charde¹,

Dhongade²,

Charde³

et al. 2012

Int Jour of Pharmc Res

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AbstractsThe present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of Curcuma longa Linn rhizomes. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200µg/ml to 1000 µg/ml shows activity in range of 9.34% to 18.55%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition and better alignment, with the obtained results it can be concluded that Curcuma longa extract has significant wound healing activity and initial healing may be due to presence of some terpenoids and antimicrobial agents. The extract shows prominent anti-inflammatory activity as compared to that of standard (Ibuprofen gel). The extract shows good anti-inflammatory activity on carageenan induced rat paw odema method.

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“…Several methods have been developed to separate the curcuminoids: thin-layer chromatography (TLC) [8] supercritical fluid chromatography (SFC) [9], gas chromatography (GC) [10], high performance liquid chromatography (HPLC) [11,12], and HPLC-mass spectrometry (LC-MS) [10,13]. Heath [14] developed a simple HPLC method for the analysis of curcumin in plasma and urine, only curcumin was determined and the other two active constituents were not mentioned.…”

Section: Introductionmentioning

confidence: 99%

Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine

Yuan

Weng

Zhang

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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Characterization of powdered turmeric by liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry

Cited by 97 publications

References 17 publications

Curcuminoids in Turmeric Roots and Supplements: Method Optimization and Validation

Curcuminoids in Turmeric Roots and Supplements: Method Optimization and Validation

Potential of Ethanolic Extract of Ficus Benghalensis on Open Wounds and Inflammation

Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine

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