Characterization of Sargassum patens C. Agardh Enzymatic Extracts Using Crude Enzyme from Shewanella oneidensis PKA 1008 and Their Anti-inflammatory Effects

Lee, Jieun; Xu, Xiaotong; Jeong, So-Mi; Kang, Woo-Sin; Ryu, Si-Hyeong; Kim, Hanho; Kim, Su-Ryong; Lee, Ga-Hye; Kim, Minji; Ahn, Dong-Hyun

doi:10.1007/s12257-021-0131-0

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…The secretion of IL-1β was shown to decrease by approximately 62% in 12 h incubation samples. In addition, in a study by Lee et al (2021), the secretion of IL-6, TNF-α, and IL-1β cytokines was significantly inhibited in a concentration-dependent manner when S. patens C. Agardh enzymatic extracts were used from 0.1, 1, 10, 50, or 100 μg/mL. Therefore, we observed that crude enzyme from S. oneidensis PKA 1008 could degrade the polysaccharides of S. fulvellum to get alginic acid oligosaccharide, which could inhibit the LPS-induced IL-6, TNF-α, and IL-1β production in RAW 264.7 cells.…”

Section: Inhibitory Effect On Production Of Lps-induced Il-6 Tnf-α An...mentioning

confidence: 97%

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Xu¹,

Jeong²,

Lee³

et al. 2022

IFRJ

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Alginic acid is a polysaccharide obtained from brown algae, and its oligosaccharide has various functions such as antiviral, antitumor, immunoregulation, and antioxidant. However, because of its high viscosity, numerous studies have degraded the alginic acid by enzymes to improve its utilisation. In the present work, we characterised Sargassum fulvellum enzymatic extract (SFEE) using polysaccharide-degrading enzyme obtained from Shewanella oneidensis PKA 1008, and investigated its anti-inflammatory potential. S. fulvellum powder and crude enzyme were mixed at a ratio of 1:1 (v/v), and reacted at 30°C for 0 - 48 h to obtain the optimum degrading time. The changes in pH, colour, reducing sugar, and viscosity of SFEE were determined. The anti-inflammatory activity of SFEE was confirmed by measuring the expression level of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, TNF-α, and L-1β) in RAW 264.7 macrophage cell line. The reducing sugar content was found to increase 2.75-fold at 24 h as compared to that at the initial reaction point, but pH and viscosity decreased significantly with increasing reaction time. SFEE showed a high inhibitory effect on the levels of NO and pro-inflammatory cytokines. SFEE thus has great potential for development as a functional food and therapeutic material owing to its anti-inflammatory effect.

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Section: Inhibitory Effect On Production Of Lps-induced Il-6 Tnf-α An...mentioning

confidence: 97%

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Xu¹,

Jeong²,

Lee³

et al. 2022

IFRJ

Self Cite

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show abstract

Properties and anti-inflammatory effects of Sargassum muticum enzymatic extracts decomposed using crude enzyme from Shewanella oneidensis PKA1008

Lee

Xu²,

Jeong³

et al. 2022

Food Sci Biotechnol

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A concise review of the highly diverse genus Sargassum C. Agardh with wide industrial potential

et al. 2023

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Characterization of Sargassum patens C. Agardh Enzymatic Extracts Using Crude Enzyme from Shewanella oneidensis PKA 1008 and Their Anti-inflammatory Effects

Cited by 4 publications

References 29 publications

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Properties and anti-inflammatory effects of Sargassum muticum enzymatic extracts decomposed using crude enzyme from Shewanella oneidensis PKA1008

A concise review of the highly diverse genus Sargassum C. Agardh with wide industrial potential

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