Characterization of serotypes and virulence genes of Haemophilus parasuis isolates from Central Vietnam

Chào, Nguyễn Văn; Thanh, Tam Vu Thi; Zou, Geng; Jia, Ming; Wang, Qiaona; Zhang, Lijun; Ding, Wenge; Huang, Qi; Zhou, Rui

doi:10.1016/j.vetmic.2019.02.008

Cited by 10 publications

(10 citation statements)

References 25 publications

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“…The serovars' overall distribution varied slightly among countries, and four serovars (1, 4, 5/12, and 7) accounted for half of all isolates. Serovars 4 and 5/12, in particular, were consistently represented across all regions in this study and previous studies [13,[22][23][24][25][26][27][28].…”

Section: Discussionsupporting

confidence: 85%

“…The serovars of utmost clinical significance were identified in samples from internal sites (serovars 4, 5/12, 1, 7, and 13) in this study. These serovars have been previously considered disease-associated [12,13,[22][23][24][25][26][27][28][29][30]. Variable sample sizes limited our ability to discern significant differences in serovar prevalence at the country level.…”

Section: Discussionmentioning

confidence: 99%

“…Data for Chinese isolates ( n = 167) and Vietnamese isolates ( n = 56) were obtained from Huazhong Agricultural University Veterinary Diagnostic Laboratory. The Chinese and Vietnamese isolates were firstly identified with biochemical tests [ 23 ] and confirmed by 16S rRNA PCR [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

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Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR

Macedo

Gottschalk

Strutzberg-Minder

et al. 2021

Vet Res

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Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly detected. Serovars of greatest clinical relevance were systemic isolates that had a higher probability of being serovar 5/12, 13, or 7. In comparison, pulmonary isolates had a higher likelihood of being serovars 2, 4, 7, or 14. Serovars 5/12 and 13 have previously been considered disease-associated, but this study agrees with other recent studies showing that serovar 7 is indeed associated with systemic G. parasuis disease. Serovar 4 strains illustrated how isolates can have varying degrees of virulence and be obtained from pulmonary, systemic, or nasal sites. Serovars 8, 9, 15, and 10 were predominantly obtained from nasal samples, which indicates a limited clinical significance of these serovars. Additionally, most internal G. parasuis isolates were classified as virulent by LS-PCR and were disease-associated isolates, including serovars 1, 2, 4, 5/12, 7, 13, and 14. Isolates from the nasal cavity, including serovars 6, 9, 10, 11, and 15, were classified as non-virulent by LS-PCR. In conclusion, the distribution of G. parasuis serovars remains constant, with few serovars representing most of the strains isolated from affected pigs. Moreover, it was confirmed that the LS-PCR can be used for G. parasuis virulence prediction of field strains worldwide.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR

Macedo

Gottschalk

Strutzberg-Minder

et al. 2021

Vet Res

Self Cite

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“…As in many other countries in Europe and all over the world [6,7,12,13,[23][24][25][26][27][28], serovar 4 is strongly represented in the present study with a portion of approximately 25% of our isolates belonging to this serovar. Similarly to recent studies from Northern Italy, the UK and Southern China [12,13,23], serovar 4 is the most important serovar in our collection, too, whereas in other countries serovar 5 (or serotype 5/12 respectively) appears to be more frequent [7,24,27,28]. However, eight other serovars (namely 13, 6, 1, 12, 7, 2, 9, and 5) were detected at frequencies of 12.3-5.2% each, and only serovar 3 was not isolated at all.…”

Section: Serotypingsupporting

confidence: 72%

Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods

Schuwerk

Hoeltig

Waldmann

et al. 2020

Vet Res

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Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glässer´s disease isolated 2012–2019 in Germany was analysed. Isolates were characterized for serovar implementing two different PCR methods. Additionally, two different PCR methods for pathotyping isolates were applied to the collection and results compared. Serovar 6 (p < 0.0001) and 9 (p = 0.0007) were correlated with carrier isolates and serovar 4 was associated with isolates from animals with respiratory disease (p = 0.015). In systemic isolates, serovar 13 was most frequently detected (18.9%). Various other serovars were isolated from all sites and the ratio of serovar 5 to serovar 12 was approximately 1:2. These two serovars together represented 14.3% of the isolates; only serovar 4 was isolated more frequently (24.7%). The pathotyping method based on the leader sequence (LS = ESPR of vta) was easy to perform and corresponded well to the clinical background information. Of the carrier isolates 72% were identified as non-virulent while 91% of the systemic isolates were classified as virulent (p < 0.0001). Results of the pathotyping PCR based on 10 different marker genes overall were in good agreement with clinical metadata as well as with results of the LS-PCR. However, the pathotyping PCR was more complicated to perform and analyze. In conclusion, a combination of the serotyping multiplex-PCR and the LS-PCR could improve identification of clinically relevant G. parasuis isolates, especially from respiratory samples.

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“…According to the research on the serotype of H. parasuis, the most prevalent serotypes were serotype 5, followed by serotype 2 and serotype 4 in Quang Binh and Thua Thien Hue provinces in Central Vietnam (17). One study about the prevalence and characteristics of H. parasuis from healthy pigs in China from 2016 to 2017 showed that the most prevalent serovars were 7, followed by 3, 2, 11, 5/12 and 4 (18).…”

Section: Discussionmentioning

confidence: 99%

Molecular Serotyping and Resistance of Clinical Strains of Haemophilus (Glaesserella) Parasuis in Chinese Pig Farms From 2016 to 2018.

Liu¹,

Guo²,

Yu³

et al. 2021

Preprint

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Haemophilus parasuis (H. parasuis) is the etiological agent of Glässer's disease and brings great economic losses to the pig industry. The goal of our research is to reveal the serotypes of H. parasuis isolated from large-scale pig farms in China from 2016 to 2018. From 2016 to 2018, 8153 H. parasuis field strains were isolated from 14610 clinical samples of sick pigs with clinical symptoms from 26 provinces and cities of China. Among them, 1386 strains were identified as H. parasuis by PCR, and the isolation rate was 9.49%. Through multiplex PCR, we showed that type 5/12 and type 4 strains had the highest separation rate, followed by type 13 and type 14 strains. Using disk diffusion method, we found cephalosporin antibiotics and peptide antibiotics all had good inhibitory effect on H. parasuis. Our conclusion may play a positive role in the prevention and treatment of H. parasuis.

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Characterization of serotypes and virulence genes of Haemophilus parasuis isolates from Central Vietnam

Cited by 10 publications

References 25 publications

Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR

Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR

Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods

Molecular Serotyping and Resistance of Clinical Strains of Haemophilus (Glaesserella) Parasuis in Chinese Pig Farms From 2016 to 2018.

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