Characterization of side population (SP) cells in murine cochlear nucleus

Abstract

In all, 1% of cells in the CN were detected as BrdU-positive. SP cells were detected at a frequency of 4.4% and expressed stem/progenitor markers, Abcb1b, Abcg2, Sca1, Notch1, Notch4, Hes1, and Jag1 in microarray analysis. Expression of Abcb1b, Abcg2, Sca1,Oct3/4, and Sox2 as determined by RT-PCR was supported by the microarray data. CN cells also had sphere-forming activity in young mice, but this activity was decreased by aging.

Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus

“…The occurrence of postnatal neurogenesis confirms recent observations of neural progenitor cells in the CN identified by neurosphere assays (18)(19)(20). We also readily observed sphere formation from all three CN subdivisions when tested at P2, although the dorsal subdivision contained significantly fewer cells with this ability (Fig.…”

“…Although the marker NeuN labels the vast majority of neurons in the CN, a few large neurons in the dorsal CN are NeuN-negative (14), which suggests that we might even underestimate the number of newborn neurons in the dorsal part of the CN. Overall, the finding of newly generated neurons combined with recent observations that the neonatal CN harbors cells with progenitor features (18)(19)(20) spurred our interest to investigate the characteristics of CN neural progenitor cells.…”

Aldehyde Dehydrogenase 1 Identifies Cells with Cancer Stem Cell-Like Properties in a Human Renal Cell Carcinoma Cell Line

“…In our present study, in order to identify the SP cells in ACHN and KRC/Y we used Hoechst staining at a concentration of 5 µg/mL and 10 µg/mL, respectively. Hoechst staining is generally carried out at a concentration of 5 µg/mL, but in the present study we used a higher concentration in KRC/Y cells [36]. Thus, we cannot completely rule out the possibility that cellular damage due to Hoechst staining was responsible for the difference in biological characteristics observed between KRC/Y cells in vivo and in vitro in our current study.…”

Cochlear nucleus whole mount explants promote the differentiation of neuronal stem cells from the cochlear nucleus in co-culture experiments

“…Cells with similar characteristics have recently been discovered in the cochlear nucleus of the mouse. These cells showed dependency in their neurogenic potential by the afferent input and were regulated by Wnt, Notch and Tgfß signalling (Ooka et al, 2012;Volkenstein et al, 2013). Thus, activation of endogenous NSCs in the cochlear nucleus is a possible treatment strategy for hearing loss.…”