Characterization of silica-based reversed-phase columns with respect to retention selectivity

Antle, P.E.; Goldberg, A.P.; Snyder, Lloyd R.

doi:10.1016/s0021-9673(01)90420-0

Cited by 81 publications

(16 citation statements)

References 34 publications

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“…Such materials can be used effectively in the purification of synthetic peptides in order to meet FDA purity expectations. Thus, in order to complement traditionally employed alkyl (generally C 8 , C 18 groups 1, 2) functional groups, developments in silica surface modification over the past two decades or so have included polar‐embedded phases 3–37, polar‐endcapped phases 15, 21, 23, 26, 28, 31–34 and phases containing phenyl groups 26, 31, 38–46, all with intent of providing alternate selectivity.…”

Section: Introductionmentioning

confidence: 99%

Reversed‐phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar‐embedded and polar endcapped columns

Mant

Cepeniene

Hodges

2010

J of Separation Science

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We desired to evaluate the chromatographic selectivity for peptides of silica-based RP high-performance liquid chromatography stationary phases with various modifications (polar embedding and polar endcapping on C(18) columns; ether-linked phenyl column with polar endcapping) compared with n-alkyl (C(18), C(8)) and aromatic phenylhexyl columns. Thus, we have designed and synthesized two series of synthetic peptide standards with the sequence Gly-Gly-Leu-Gly-Gly-Ala-Leu-Gly-X-Leu-Lys-Lys-amide, where the N-terminal either contains a free α-amino group (AmC series) or is N(α)-acetylated (AcC series) and where position X is substituted by Gly, Ala, Val, Ile, Phe or Tyr. These represent series of peptides with single substitutions of n-alkyl (Gly

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Section: Introductionmentioning

confidence: 99%

Reversed‐phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar‐embedded and polar endcapped columns

Mant

Cepeniene

Hodges

2010

J of Separation Science

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“…A sophisticated test employing gradient elution was reported by Antle et al [31], who described a relative columnstrength parameter (J), which was shown to be a function of the stationary phase volume, surface area and polarity and was evaluated relative to an arbitrarily selected standard column. A sophisticated test employing gradient elution was reported by Antle et al [31], who described a relative columnstrength parameter (J), which was shown to be a function of the stationary phase volume, surface area and polarity and was evaluated relative to an arbitrarily selected standard column.…”

Section: Introductionmentioning

confidence: 99%

Characterization of different RP-HPLC columns by a gradient elution technique

et al. 1997

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The literature contains no unified testing procedure for evaluation and characterization of reversed-phase (RP) columns of different pore sizes (surface area) and containing different types of ligand. In our laboratory a testing procedure has been developed using gradient elution under standardized conditions independent of column dimensions and applicable to both narrow-pore and wide-pore columns. Six wide-pore and four narrowpore columns were investigated and compared. The test solutes were selected to cover a wide range of chemical properties. The evaluation and characterization of the columns was performed on the basis of kapp retention factors. Principal components analysis (PCA) was performed to reveal similarities and differences among the columns and test substances. The factors obtained characterize the columns according to the extent of various interactions such as hydrophobicity, HB (hydrogen bond)-donor acidity and HB-acceptor basicity.

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“…Antle et al. showed that the differences in solute retention is correlated with the effective phase ratio of the column, the “polarity” of the bonded phase and the dispersion solubility parameter of the stationary phase. The phase ratio of the column is a function of the chain length, the bonding density, the surface area of the silica and the pore distribution.…”

Section: Methodsmentioning

confidence: 99%

Retention behavior of resorcinarene‐based cavitands on C₈ and C₁₈ stationary phases

Bartó

Prauda

Kilár

et al. 2015

J of Separation Science

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The understanding of the retention behavior of large molecules is an area of interest in liquid chromatography. Resorcinarene-based cavitands are cavity-shaped cyclic oligomers that can create host-guest interactions. We have investigated the chromatographic behavior of two types of cyclic tetramers as analytes in high-performance liquid chromatography. The experiments were performed at four different temperatures (15, 25, 35, 45°C) on two types of reversed stationary phases (C8 and C18 ) from two different manufacturers. We have found a huge difference between the retention of resorcinarenes and cavitands. In some cases, the retention factor of cavitands was even a hundred times larger than the retention factor of resorcinarenes. The retention of methylated derivates was two to four times larger compared to that of demethylated compounds on every column. The opposite retention behavior of the resorcinarenes and cavitands on the two types of stationary phases showed well the difference of the selectivity of the XTerra and BDS Hypersil columns. The retention mechanism was studied by the thermodynamic parameters calculated from the van't Hoff equation.

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Characterization of silica-based reversed-phase columns with respect to retention selectivity

Cited by 81 publications

References 34 publications

Reversed‐phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar‐embedded and polar endcapped columns

Reversed‐phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar‐embedded and polar endcapped columns

Characterization of different RP-HPLC columns by a gradient elution technique

Retention behavior of resorcinarene‐based cavitands on C₈ and C₁₈ stationary phases

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Characterization of silica-based reversed-phase columns with respect to retention selectivity

Cited by 81 publications

References 34 publications

Reversed‐phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar‐embedded and polar endcapped columns

Reversed‐phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar‐embedded and polar endcapped columns

Characterization of different RP-HPLC columns by a gradient elution technique

Retention behavior of resorcinarene‐based cavitands on C8 and C18 stationary phases

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Retention behavior of resorcinarene‐based cavitands on C₈ and C₁₈ stationary phases