Characterization of spelt (Triticum spelta L.) forms by gel electrophoretic analyses of seed storage proteins. I. The gliadins

Harsch, S.; Günther, Thomas; Rozynek, B.; Hesemann, C. U.; Kling, C. I.

doi:10.1007/s001220050381

Cited by 24 publications

(19 citation statements)

References 23 publications

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“…Combined together, ω-and γ-gliadins give 35 different combinations in our study. The variation found among the analysed accessions is higher than that found in other materials, thus Harsch et al (1997) found 30 bands in sixteen spelt wheat cultivars. Sewa et al (2005) reported 45 different gliadin bands in 157 Indian wheats, Caballero et al (2004) Diversity index.…”

Section: Resultscontrasting

confidence: 65%

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The genetic diversity of gliadins in Aegilops geniculata from Algeria

Medouri¹,

Bellil²,

Khelifi³

2015

CZECH J GENET PLANT

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Medouri A., Bellil I., Khelifi D. (2015): The genetic diversity of gliadins in Aegilops geniculata from Algeria. Czech J. Genet. Plant Breed., 51: 9-15.The gliadins of the wild wheat Aegilops geniculata represent a valuable gene pool in breeding for bread making quality. The genetic diversity of gliadins in A. geniculata was studied among 36 of its accessions, collected in the north of Algeria, using acid polyacrylamide-gel electrophoresis (Acid-PAGE). In total, sixty-one polymorphic bands and 35 gliadin patterns were identified. Twenty-eight different bands and 34 patterns were found in the ω-gliadin region, 13 polymorphic bands and 33 patterns for γ-gliadins, 12 bands and 34 different patterns for β-gliadins and eight bands in combination resulted in 25 different patterns in the α-gliadin zone. Thirty-five patterns were found for each of the Gli-1 (γ/ω region) and Gli-2 (α/β region) loci. The genetic diversity index (H) was higher for ω-gliadins (0.968), followed by γ-and β-gliadins (0.964 and 0.961, respectively), and the lowest value was detected in α-gliadin patterns (0.944). Cluster analysis based on Ward's method divided the analysed collection into five separated groups in which genetic diversity did not follow the geographical distribution. The polymorphism observed in the electrophoretic patterns highlights close correlations between bioclimatic features and some ω-gliadin proteins.

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Section: Resultscontrasting

confidence: 65%

“…Also Harsch et al (1997) found 30 different bands only when analysing 16 spelt cultivars using densitometry methods.…”

Section: Resultsmentioning

confidence: 98%

The genetic diversity of gliadins in Aegilops geniculata from Algeria

Medouri¹,

Bellil²,

Khelifi³

2015

CZECH J GENET PLANT

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“…The similar electrophoretic characterisation of spelt seed gliadins was studied by several authors (Abdel-Aal et al 1996, Harsch et al 1997, aek and Èerný 1997. HMW glutenin subunits may be used as a molecular marker of bread-making quality of wheat.…”

Section: Resultsmentioning

confidence: 81%

Variation in HMW glutenin subunits of different species of wheat

Gálová¹,

MichalíkI²,

Knoblochová³

et al. 2002

PLANT SOIL ENVIRON

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Method ISTA SDS-PAGE was used for separation, detection and evaluation of high molecular weight glutenin subunits (HMW) in the different wheat species. The relation has been studied between the HMW glutenin subunit alleles and the bread-making quality of 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. The highest frequency of occurrence of HMW glutenin subunits 2*, 13 + 16 and 5 + 10 were found in world wheat cultivars. In Slovak wheat varieties were analysed subunits 0, 7 + 9 and 5 + 10, 2 + 12. The HMW subunits 0, 7 + 8 with Glu-score 4 were determined in Triticum durum DESF. Three electrophoretical profile groups of different HMW glutenin subunits were found in Triticum turgidum L. and Triticum polonicum L. and six electrophoretical profile groups were determined in Triticum spelta L. The verified correlations between bread-making quality and specific HMW subunits of glutenin can be utilised by wheat breeders using SDS-PAGE of proteins as a screening test for the prediction of bread-making quality of wheat. The glutenin proteins of wheat, particularly the highly aggregative glutenin fraction (HMW) strongly influence the bread-making properties of flours. The HMW glutenin subunits are encoded by genes at three genetically unlinked loci Glu-A1, Glu-B1, and Glu-D1, located close to the centromere on the long arm of the homoeologous chromosomes 1A, 1B, and 1D, respectively. There is the significant relationship between the presence and the absence of some specific HMW glutenin subunits and the bread-making quality of wheat varieties (Payne and Lawrence 1983, Payne et al. 1987, Vlasák 1995, aek and Èerný 1997. The most important subunits are 5 + 10 and 2 + 12, which are expressed from Glu-D1 loci. Method of SDS-PAGE is possible to apply for separation, detection and evaluation of high molecular weight glutenin subunits in wheat. KeywordsThe aim of our present contribution was to determine the type of the HMW glutenin subunits and to calculate the Glu-score in the grain of different species of wheat Triticum aestivum L., Triticum spelta L., Triticum durum DESF., Triticum turgidum L. and Triticum polonicum L. MATERIAL AND METHODSThe relationship between the HMW glutenin subunit alleles and the bread-making quality of different wheat species has been studied. The winter wheats included 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. which were obtained from the Slovak Republic Genbank and from the Czech Republic Genbank. The cultivars Neepawa, Chinese spring, and Marquist were used as standards.For identification and characterisation of wheat varieties the standard vertical disc...

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“…Seed storage proteins are highly polymorphic, largely independent of environmental factors and have been widely used for identification of genetic similarity and diversity (8,9,21). Therefore, the seed storage protein profiling of germplasms is used for several purposes such as legal plant cultivar protection, registration, certification, cultivar purity testing, crop origin and evolutionary studies (5,10,26,30).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Genetic Similarity and Agronomic Traits of Castor Bean Populations Naturally Grown in the Eastern Mediterranean Region of Turkey

Arslan

2012

Biotechnology & Biotechnological Equipment

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Characterization of spelt (Triticum spelta L.) forms by gel electrophoretic analyses of seed storage proteins. I. The gliadins

Cited by 24 publications

References 23 publications

The genetic diversity of gliadins in Aegilops geniculata from Algeria

The genetic diversity of gliadins in Aegilops geniculata from Algeria

Variation in HMW glutenin subunits of different species of wheat

Evaluation of Genetic Similarity and Agronomic Traits of Castor Bean Populations Naturally Grown in the Eastern Mediterranean Region of Turkey

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