Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

Plieva, Fatima M.; Savina, Irina N.; Deraz, Sahar F.; Andersson, Jonatan; Galaev, Igor Yu.; Mattìasson, Bo

doi:10.1016/j.jchromb.2004.01.050

Cited by 159 publications

(117 citation statements)

References 6 publications

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“…For example, co-polymerisation with allyl glycidyl ether (AGE) is often used to introduce the epoxy functionality for further conversion into anion-exchange or chelating group or immobilisation of a bio-ligand. However, an increase in the allyl glycidyl ether content results in the formation of brittle and non-elastic cryogels [41]. Co(polyacrylamide-allyl glycidyl ether) cryogels become brittle when concentration of AGE exceeds 20%.…”

Section: Cryotropic Gelation Features and The Nature Of Freezing Processmentioning

confidence: 99%

“…Therefore, determination of the amounts of different types of water in cryogels [50,51,54] is of importance to understand the nature of adsorption/desorption and diffusion processes within the pore network in cryogels, as well as to estimate the biocompatibility and structural organisation of whole cryogels [2][3][4][10][11][12][13][14][15][16][17][18][19][20][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49]54]. A variety of experimental methods sensitive to the water state can be used for these purposes [50][51][52][53][54][55][56].…”

mentioning

confidence: 99%

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Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

288

205

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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Section: Cryotropic Gelation Features and The Nature Of Freezing Processmentioning

confidence: 99%

mentioning

confidence: 99%

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

288

205

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“…SEM clearly demonstrated the formation of newlyformed polymer phase inside the cryogel pores ( Figure 3, for structure of non-grafted pAAm cryogel see Figure 2 in Plieva et al [23]). The grafted polymer is visible in the form of particles of different shape and size, which are unevenly distributed over the polymer surface of the cryogel walls (Figure 3).…”

Section: Resultsmentioning

confidence: 94%

“…Cryogels are pro- duced by radical copolymerization of acrylamide with methylene-bis-acrylamide in partially frozen reaction media [22,23]. They are characterized by dense non-porous polymer walls and a system of large interconnected pores filled with water.…”

Section: Resultsmentioning

confidence: 99%

Analysis of polymer grafted inside the porous hydrogel using confocal laser scanning microscopy

Tuncel¹,

Tuncel²,

Galaev³

et al. 2007

Express Polym. Lett.

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Abstract. Graft polymerization of glycidyl methacrylate onto the pore surface of polyacrylamide macroporous gel was implemented in DMSO-aqueous solution using diperiodatocuprate(III) complexes as an initiator. The grafting densities up to 410% were achieved. The graft polymerization was confirmed by gravimetrical methods and FTIR. The graft polymerization of polymer inside the pores of the macroporous gel resulted in increased flow resistance through the gel matrix. The distribution of grafted polymer on the gel pore surface material was studied by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). CLSM is an alternative method for studying morphology of gel surface with grafted polymer having the advantages over the SEM allowing to investigate the distribution of grafted polymer inside the hydrogel in a native hydrated state. The microscopic techniques demonstrated uneven distribution of the grafted polymer inside the gel pores as a result of initiating the graft polymerization by insoluble initiator deposited on the pore surface.

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“…The water content of cryogel disks were determined according to the studies of Plieva et al, and Horak et al 19,20,21 Dried disks were mounted into chamber saturated with water vapor. Water vapor adsorbed onto cryogel disk by time was determined and then weight of cryogel disks adsorbed water vapor was measured (mwater bound disk).…”

Section: Characterization Of the Poly(hema) Cryogel Disksmentioning

confidence: 99%

Cadmium Removal Performances of Different Dye Ligands Attached Cryogel Disks

Keleş¹,

Inanan²,

Tüzmen³

et al. 2015

Croat. Chem. Acta

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Abstract. Poly(HEMA) cryogel disks were synthesized by free radical polymerization of 2-hydroxyethylmethacrylate (HEMA), and then Cibacron Blue F3GA (CB), Reactive Green 19 (RG) and Congo Red (CR) were immobilized as dye ligands. Disks were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and swelling degree, porosity calculations. Cd 2+ adsorption experiments were performed for 60 min. Maximum adsorption capacities were determined as 25.5 mg/g; 48.0 mg/g and 28.5 mg/g at pH = 7.0 for poly(HEMA)-CB; poly(HEMA)-RG and poly-(HEMA)-CR, respectively. Langmuir isotherm fitted best with the adsorption data and adsorption thermodynamics showed that Cd 2+ adsorption is thermodynamically favorable and a physisorption process. A great majority of adsorbed Cd 2+ desorbed with 1 M NaCl and cryogel disks can be re-used in adsorption experiments. Cd 2+ removal efficiencies of disks from human plasma are approximately 45 %. Dyeattached cryogel disks synthesized in this study have potential in use for environmental and therapeutic applications.

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Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

Cited by 159 publications

References 6 publications

Cryogels: Morphological, structural and adsorption characterisation

Cryogels: Morphological, structural and adsorption characterisation

Analysis of polymer grafted inside the porous hydrogel using confocal laser scanning microscopy

Cadmium Removal Performances of Different Dye Ligands Attached Cryogel Disks

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