Characterization of the Antibacterial Activity and the Chemical Components of the Volatile Oil of the Leaves of Rubus parvifolius L.

Cai, Yongqing; Hu, Xiaogang; Huang, Mingchun; Sun, Fengjun; Yang, Bo; Ju-ying, HE; Wang, Xianfeng; Xia, Peiyuan; Chen, Jianhong

doi:10.3390/molecules17077758

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…Volatile organic compounds in the extracts were analyzed by using the GC-MS method previously established by Cai and coworkers [16-18]. Conditions included a capillary column (Model Number 19091S-433, HP-5MS, 30 m x 0.25 mm i.d., film thickness 0.25 μm, Agilent Technologies, Santa Clara, CA, USA), sample volume (0.2 mL), helium gas used as a carrier gas with a flow rate of 1.0 mL/min, and mass spectra obtained by electron ionization at 70 eV and detected in a range of 30 - 300 m/z.…”

Section: Methodsmentioning

confidence: 99%

“…In assay, the extracts (50, 100 and 200 µg/mL) were incubated with working ABTS •+ solution for 15 min and the absorbance measured at 734 nm. ABTS • scavenging capacity of the extracts was calculated by comparing with a standard curve of trolox (0 - 200 µg/mL) [17, 18]. The percentage of inhibition was calculated using the following equation (2):…”

Section: Methodsmentioning

confidence: 99%

“…Next, LPS (2 µg/mL) was added to the wells and incubated for 24 h [20]. After centrifugation, the supernatant media were incubated with Griess reagent composing 0.1% (w/v) naphthyl-ethylene-diamide dihydrochloride, 1% (w/v) sulfanilamide and 5% (v/v) concentrated phosphoric acid and measured absorbance at 650 nm [18]. Produced NO • concentration was calculated from the standard curve of sodium nitrite (0 - 200 nM).…”

Section: Methodsmentioning

confidence: 99%

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Anti-inflammatory and Antioxidant Activities of the Extracts from Leaves and Stems of Polygonum odoratum Lour

Chansiw

Chotinantakul

Srichairatanakool

2019

AIAAMC

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Background: Polygonum odoratum is an indigenous vegetable that has been used as a favoring agent and also used as a Thai traditional medicine to treat flatulence. Objective: To analyze active ingredients, total phenolic and total flavonoid contents, antiinflammatory and antioxidant activities from leaf and stem extracts of P. odoratum. Methods: Leaves and stems were dried and extracted by using methanol, dichloromethane and water for obtaining Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MTE), Dichloromethane Leaf Extract (DLE), Dichloromethane Stem Extract (DTE), Water Leaf Extract (WLE) and Water Stem Extract (WTE). The extracts were quantified for total phenolic and total flavonoid contents by spectrophotometry and active compounds were analyzed by using GC-MS. Antioxidant activity was determined by ABTS and DPPH radicals scavenging assays. Anti-inflammatory activity was tested by the inhibition of nitric oxide production in RAW 264.7 macrophage cells induced by lipopolysaccharide. Results: The DLE exhibited the most potent anti-inflammatory effect by inhibiting nitric oxide production in a concentration-dependent manner (IC50 = 53.75±0.72 µg/mL). MLE exhibited strong antioxidant activity and contained the highest concentration of phenolic compounds (52.59±0.58 mg gallic acid equivalent/g extract) and flavonoid (19.97+0.11 mg quercetin equivalent/g extract). E-15-Heptadecenal and 3, 7, 11, 15-tetramethyl-2- hexadecen-1-ol were found predominantly in the methanol extracts. Conclusion: The leaf extract of P. odoratum showed potent anti-inflammatory and antioxidant activities, mediated by DLE and MLE, respectively.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Anti-inflammatory and Antioxidant Activities of the Extracts from Leaves and Stems of Polygonum odoratum Lour

Chansiw

Chotinantakul

Srichairatanakool

2019

AIAAMC

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“…Although no reports on volatile metabolites from R. ideaus leaves have been published, other Rubus species have been phytochemically investigated for their volatile compounds. Cai et al [32] analyzed the components contained in the hydro-distillated volatile oil from R. parvifolius L. leaves by GC/MS. They identified 29 compounds, with 4-hydroxy-3metoxystirene being the most abundant one (66.05%), followed by 2-hexadecen-1-ol (9.79%), 4-tert-butyl benzoic acid (2.22%), hexanoic acid (2.03%), and linalool (1.39%).…”

Section: General Aspectsmentioning

confidence: 99%

Bioactivity and Chemical Profile of Rubus idaeus L. Leaves Steam-Distillation Extract

Santis

Carbone

Garzoli

et al. 2022

Foods

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The leaves of Rubus idaeus L., a by-product of the fruit food industry, are a known source of bioactive molecules, although the chemical composition has only been partially investigated. The main objective of this study was to examine the biological activities and the chemical composition of the extract of leaves of R. idaeus (RH), obtained by steam distillation (SD). The antioxidant capacity; the total phenolic content (TPC); the cytotoxic activity against tumor cell lines; and the antibacterial activity, in addition to the study of the chemical fingerprinting, carried out by Gas/Chromatography-Mass-Spectrometry (GC/MS) and Headspace (HS)-GC/MS, were established. The extract showed a strong antioxidant capacity and a modest antibacterial activity against two bacterial strains, as well as significant cytotoxic activity against tumor cell lines (Caco-2 and HL60) and being proliferative on healthy cells. Many of the GC-identified volatile molecules (1,8-cineol, β-linalool, geraniol, caryophyllene, τ-muurolol, citral, α-terpineol, 3- carene, α-terpinen-7-al, etc.) can explain most of the biological properties exhibited by the extract of R. idaeus L. The high biological activity of the RH and the high compatibility with the various matrices suggest good prospects for this extract, both in the food and cosmetic fields or in dietary supplements for improving human health.

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“…In an in vitro experiment, Seleshe et al showed that RP has the antioxidant and antimicrobial effects [ 17 ]. The study of Cai et al exhibited that RP showed an antibacterial effect in vitro [ 22 ]. In in vivo animal experiment, Zhang et al reported that RP can inhibit the growth of leukemia K562 cells in vitro and in vivo animals [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Total saponins from Rubus parvifolius L. inhibits cell proliferation, migration and invasion of malignant melanoma in vitro and in vivo

Cao

Zhao

et al. 2021

Bioscience Reports

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Background: Total saponins from Rubus parvifolius L. (TSRP) is the main bioactive fractions responsible for the antitumor activities. The work was aimed to evaluate the anti-tumor effect of TSRP in malignant melanoma in vitro and in vivo. Methods and Results: Anti melanoma cell proliferation, invasion and migration effect of TSRP were detected in human malignant melanoma A375 cells under the indicated time and dosages. In vivo anti-tumor effect of TSRP was measured in A375 xenograft immunodeficient nude mice. Sixty A375 xenografts were randomly divided into five groups: Vehicle, cyclophosphamide (CTX, 20 mg/kg), TSRP (25 mg/kg), TSRP (50 mg/kg) and TSRP (100 mg/kg) groups for 14 days’ treatment. In addition, the melanoma cell metastasis in lung in vivo of TSRP was detected in A375 tail vein injection mice, and the histopathalogical analysis of the metastasis lung was detected by H & E stating. TSRP was significantly inhibited the cell proliferation, invasion and migration of A375 in vitro at the indicated time and dosages. TSRP treatment was effectively blocked the tumor growth in immunodeficient nude mice. In addition, TSRP was also significantly inhibited the melanoma metastasis of lung. Conclusion: This study indicated that the TSRP has a remarkable anti malignant melanoma effect, which mainly through the inhibition of the cell invasion,migration and tumor metastasis.

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Characterization of the Antibacterial Activity and the Chemical Components of the Volatile Oil of the Leaves of Rubus parvifolius L.

Cited by 10 publications

References 13 publications

Anti-inflammatory and Antioxidant Activities of the Extracts from Leaves and Stems of Polygonum odoratum Lour

Anti-inflammatory and Antioxidant Activities of the Extracts from Leaves and Stems of Polygonum odoratum Lour

Bioactivity and Chemical Profile of Rubus idaeus L. Leaves Steam-Distillation Extract

Total saponins from Rubus parvifolius L. inhibits cell proliferation, migration and invasion of malignant melanoma in vitro and in vivo

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