Characterization of the chicken preprogonadotrophinreleasing hormone-I gene

Abstract

Partial cDNA clones for chicken gonadotrophin-releasing hormone (GnRH)-I were isolated by reverse transcription-polymerase chain reaction using total RNA from the hypothalami of domestic chickens. Primers for amplification were based on the nucleotide sequence of the mammalian GnRH genes. These amplified clones were used to screen a genomic library from which a series of overlapping clones was isolated. A 6.3 kb EcoRI fragment containing all the exons and 3.0 kb of the 5' upstream region was sequenced. The exon-intron structure of the gene was found to be of a similar configuration to those of the mammalian and osteichthyes GnRH genes analysed so far. Individual domains of the predicted prepropeptide are similar to those of mammalian GnRH prepropeptides, comprising a 23 amino acid signal peptide, the decapeptide hormone and a Gly-Lys-Arg cleavage site, followed by a 56 amino acid GnRH-associated peptide. The nucleotide sequence coding for the decapeptide hormone translates into the amino sequence for chicken GnRH-I. The prepropeptide has approximately 50% identity with mammalian prepropeptides and 25% identity with the teleost prepropeptides.

Molecular cloning, expression pattern, and immunocytochemical localization of a gonadotropin-releasing hormone-like molecule in the gastropod mollusk, Aplysia californica

“…First, they both share the common molecular architecture of a signal peptide, a GnRH dodecapeptide, followed by a GAP. This general architecture is also observed in chordate prepro-GnRHs (Seeburg and Adelman, 1984;Suzuki et al, 1992;Dunn et al, 1993;Hayes et al, 1994;Adam et al, 2003). Second, a moderate level of similarity (37.2%) exists in the GAP regions, but the greatest sequence similarity exists in the GnRH dodecapeptide region (83.3%; Fig.…”

A study of association between genetic markers in candidate genes and reproductive traits in one generation of a commercial broiler breeder hen population

“…Despite using 19 primer pairs covering 46% of the 6.4-kb of the known GNRH gene sequence and its upstream region (Dunn et al, 1993), no polymorphic sites were detected in the 30 individuals.…”

Isolation, characterization and expression of cDNAs encoding the catfish‐type and chicken‐II‐type gonadotropin‐releasing‐hormone precursors in the African catfish

“…The amino acid sequences of the two catfish gonadotropin-releasing-hormone precursors and the chicken gonadotropin-releasing-hormone-I1 precursor of the African catfish were compared to the amino acid sequences of the salmon gonadotropin-releasing-hormone precursor of masu salmon [13], of Atlantic salmon [12] and of a cichlid [14], to the chicken gonadotropin-releasing-hormone-I precursor of chicken [22], and to the mammalian gonadotropin-releasinghormone precursor of mouse [20], of rat [21] and of human [19], respectively (Fig. 3).…”