Characterization of the complete mitochondrial genome of Ixodes granulatus (Ixodidae) and its phylogenetic implications

Lu, Xinyan; Zhang, Quanfu; Jiang, Dandan; Du, Chunhong; Xu, Rong; Guo, Xian‐Guo; Xing, Yang

doi:10.1007/s00436-022-07561-0

Cited by 3 publications

(12 citation statements)

References 31 publications

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“…Ticks, which are common hematophagous ectoparasites, are capable of parasitizing almost all groups of terrestrial vertebrates, including mammals, birds, lizards, snakes, and toads [ 1 , 2 , 3 ]. Ticks rank second in the world as vectors of disease, and they can harbor and transmit a great variety of pathogens, including virus, bacteria, fungi, protozoa, and nematode, to humans, livestock, and wildlife [ 2 , 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Correct identification of tick species is essential for the study of ticks and tick-borne pathogens [ 21 ]. The conventional method for identifying ticks is based on microscopic observation of morphological features, which is suitable for adult ticks [ 3 ]. Many tick species share great morphological similarities and therefore group into species complexes until differentiating characters can be described [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Comparative mitogenomic analyses are of great significance for understanding the biodiversity and evolution, and mitogenomic data can provide efficient phylogenetic signals for a wide variety of animal groups [ 3 , 24 , 33 , 34 ]. The phylogenetic relationships of hard ticks have been studied in depth [ 3 , 4 , 35 , 36 , 37 , 38 , 39 ], but comprehensive comparative mitogenome analyses of them are rarely performed. Herein, we sequenced the mitogenomes of the giant panda-infecting ticks I. acutitarsus and I. ovatus through high-throughput sequencing technology, and performed comparative and phylogenetic genomic analyses for the newly sequenced and publicly available mitogenomes of hard ticks.…”

Section: Introductionmentioning

confidence: 99%

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Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Liu

et al. 2022

Genes

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Ticks rank second in the world as vectors of disease. Tick infestation is one of the factors threatening the health and survival of giant pandas. Here, we describe the mitogenomes of Ixodes acutitarsus and Ixodes ovatus parasitizing giant pandas, and perform comparative and phylogenetic genomic analyses on the newly sequenced and other available mitogenomes of hard ticks. All six newly determined mitogenomes contain a typical gene component and share an ancient Arthropoda gene arrangement pattern. Our study suggests that I. ovatus is a species complex with high genetic divergence, indicating that different clades of I. ovatus represent distinct species. Comparative mitogenomic analyses show that the average A + T content of Ixodidae mitogenomes is 78.08%, their GC-skews are strongly negative, while AT-skews fluctuate around 0. A large number of microsatellites are detected in Ixodidae mitogenomes, and the main microsatellite motifs are mononucleotide A and trinucleotide AAT. We summarize five gene arrangement types, and identify the trnY-COX1-trnS1-COX2-trnK-ATP8-ATP6-COX3-trnG fragment is the most conserved region, whereas the region near the control region is the rearrangement hotspot in Ixodidae mitogenomes. The phylogenetic trees based on 15 genes provide a very convincing relationship (Ixodes + (Robertsicus + ((Bothriocroton + Haemaphysalis) + (Amblyomma + (Dermacentor + (Rhipicentor + (Hyalomma + Rhipicephalus))))))) with very strong supports. Remarkably, Archaeocroton sphenodonti is embedded in the Haemaphysalis clade with strong supports, resulting in paraphyly of the Haemaphysalis genus, so in-depth morphological and molecular studies are essential to determine the taxonomic status of A. sphenodonti and its closely related species. Our results provide new insights into the molecular phylogeny and evolution of hard ticks, as well as basic data for population genetics assessment and efficient surveillance and control for the giant panda-infesting ticks.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Liu

et al. 2022

Genes

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“…The PCR conditions used in the amplification procedure were as follows: initial denaturation at 95 • C for 5 mins, followed by 45 cycles of denaturation (98 • C for 10 s), annealing (68 • C for 30 s), and extension (68 • C for 10 mins), and a final extension was subjected to 68 • C for 10 mins. The findings of the PCR were examined using 1.2 percent agarose gel electrophoresis stained with ethidium bromide (15). Libraries were sequenced on the Illumina HiSeq 2500 platform at Shanghai Biotechnology Co. Ltd. after the amplified PCR products had been purified.…”

Section: Dna Amplification and Sequencingmentioning

confidence: 99%

Complete mitogenomes and phylogenetic relationships of Haemaphysalis nepalensis and Haemaphysalis yeni

Zhang

Jiang

et al. 2022

Front. Vet. Sci.

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The mitochondrial genome may include crucial data for understanding phylogenetic and molecular evolution. We sequenced the complete mitogenome of Haemaphysalis nepalensis and Haemaphysalis yeni for the first time. H. nepalensis and H. yeni's complete mitogenomes were 14,720 and 14,895 bp in size, respectively, and both contained two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 13 protein-coding genes (PCG). Haemaphysalis nepalensis have one control region (D-loop). The adenine + thymine concentration of the genomes of H. nepalensis and H. yeni was 77.75 and 78.41%, respectively. The codon use pattern and amino acid content of proteins were both observed to be affected by the AT bias. Genes in the mitogenome were organized and located in a comparable manner to previously known genes from Haemaphysalis ticks. Mitochondrial PCGs were used to perform phylogenetic relationships based on the Minimum Evolution (ME) approach using MEGA 7.0 software, the results reveal that H. nepalensis has tight links with H. tibetensis, H. yeni and H. kolonini share a sister group relationship, and that H. nepalensis and H. yeni belong to Haemaphysalis. The results of this study include the following: (i) discovered and supplied new tick records (H. nepalensis) for China, (ii) provided the first complete mitochondrial genome for H. nepalensis and H. yeni and revealed their phylogenetic relationships, and (iii) the features of the mitochondrial genome of H. nepalensis and H. yeni provided more genetic reference for Phylogeography, systematics, and population genetics of the Haemaphysalis species.

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Analysis of complete mitogenomes and phylogenetic relationships of Frontopsylla spadix and Neopsylla specialis

Liu,

Chen,

et al. 2023

Front. Vet. Sci.

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Fleas represent a group of paramount medical significance, subsisting on blood and acting as vectors for an array of naturally occurring diseases. These pathogens constitute essential elements within the plague biome, exerting deleterious effects on both human and livestock health. In this study, we successfully assembled and sequenced the whole mitochondrial genome of Frontopsylla spadix and Neopsylla specialis using long-range PCR and next-generation sequencing technologies. The mitogenomes of F. spadix and N. specialis both have 37 genes with full lengths of 15,085 bp and 16,820 bp, respectively. The topology of the phylogenetic tree elucidates that species F. spadix is clustered in a branch alongside other members of the family Leptopsyllidae, whereas species N. specialis is a sister taxon to Dorcadia ioffi and Hystrichopsylla weida qinlingensis. It also suggests that Pulicidae form a monophyletic clade, Ctenopthalmidae, Hystrichopsyllidae, Vermipsyllidae form a sister group to Ceratophyllidae/Leptopsyllidae group. The mitochondrial genomes of F. spadix and N. specialis were sequenced for the first time, which will contribute to a more comprehensive phylogenetic analysis of the Siphonaptera order. The foundation for subsequent systematic studies, and molecular biology of fleas was established.

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Characterization of the complete mitochondrial genome of Ixodes granulatus (Ixodidae) and its phylogenetic implications

Cited by 3 publications

References 31 publications

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Complete mitogenomes and phylogenetic relationships of Haemaphysalis nepalensis and Haemaphysalis yeni

Analysis of complete mitogenomes and phylogenetic relationships of Frontopsylla spadix and Neopsylla specialis

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