Characterization of the genomic responses in early Senegalese sole larvae fed diets with different dietary triacylglycerol and total lipids levels

Hachero‐Cruzado, Ismael; Rodríguez-Rúa, Ana; Román-Padilla, Javier; Ponce, Marian; Fernández‐Díaz, Catalina; Manchado, Manuel

doi:10.1016/j.cbd.2014.09.005

Cited by 28 publications

(36 citation statements)

References 84 publications

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“…In this work, we wanted to describe the expression of digestive enzymes in chame D. latifrons during the transition from endogenous to exogenous feeding without the influence of the diet, to establish its digestive strategy or preferred metabolic substrate. It has been suggested that feeding does not affect the expression of the genes that code for digestive enzymes during the first days of development because they are genetically programmed (Zambonino‐Infante & Cahu, ; Zambonino‐Infante et al, ), but other species such as Solea senegalensis shows an effect of the diet during the first days of life in this species (Gamboa‐Delgado et al, ; Hachero‐Cruzado et al, ) changing enzymatic activity patterns. Nevertheless, given the poor knowledge of the actual feeding habits of the larvae, in both their natural habit of this species or laboratory rearing conditions, is not known, we consider that carrying out the analyses in starvation conditions provides a baseline for future feeding trials.…”

Section: Discussionmentioning

confidence: 99%

Expression analyses of digestive enzymes during early development and in adults of the chame fishDormitator latifrons

et al. 2019

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This study describes the expression of genes that encode digestive enzymes (trypsin, pepsinogen, maltase-glucoamylase, sucrase-isomaltase, alkaline phosphatase and leucine aminopeptidase) using qRT-PCR in 0-to 7-day post-hatching (dph) chame (Dormitator latifrons). Additionally, adults liver and intestine were analysed for enzyme expression.Results showed that transcripts for all enzymes are present in both adult and larvae tissues. In adults, the expression of maltase-glucoamylase and sucrose-isomaltase showed the highest values in the middle intestine, while the liver and anterior and posterior intestine showed low expression levels. Other enzymes showed low expression in all tissues, with the exception of leucine aminopeptidase, which showed high expression in liver. In larvae whole-body samples of D. latifrons, maltase-glucoamylase and sucrose-isomaltase showed the highest expression from 3 to 5 dph, while other enzymes maintained low levels from hatching to 7 dph. The highest expression of disaccharidases such as maltaseglucoamylase and sucrose-isomaltase corresponded to the beginning of exogenous feeding, suggesting that this species exhibits an herbivorous profile. However, the presence of proteolytic enzymes may indicate that D. latifrons is programmed to be able to digest other substrates. This information will contribute to the development of larval feeding protocols to produce chame juveniles in laboratory conditions, as prior larval feeding essays have not been successful, using either live feeds or commercial fish larval diets. K E Y W O R D S chame, digestive enzyme, Dormitator latifrons, gene expression How to cite this article: Morelos-Castro, Román-Reyes JC, Luis-Villaseñor IE, Ramírez-Pérez JS, Rodríguez-Montes de Oca GA. Expression analyses of digestive enzymes during early development and in adults of the chame fish Dormitator latifrons. Aquac Res. 2020;51:265-275. https ://doi.

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Section: Discussionmentioning

confidence: 99%

Expression analyses of digestive enzymes during early development and in adults of the chame fishDormitator latifrons

et al. 2019

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“…This reference transcriptome is considered a useful tool for deep sequencing studies to minimize data processing times without losing any significant information about regulatory pathways in the functional analysis (Hachero-Cruzado et al, 2014;Fatsini et al, 2016). Sequence comparisons of the reference transcriptome with the previously published transcriptome in T. lutea (Carrier et al, 2014) indicated an FIGURE 4 | Expression profiles of sod1, sod2, sod3, aryl, ccp, and dio during the growth of T. lutea.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta)

et al. 2018

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Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related molecular processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant-(aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H 2 O 2 ) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.

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“…Therefore, although the molecular expression patterns of digestive enzymes can be used as markers for fish larval development (Lazo et al, 2011), the molecular mechanisms for the regulation of digestion are not well understood. It seems that gene expression of some digestive enzymes is genetically programmed during the first days of feeding, but a potential effect of feeding level and diet composition has also been reported (Gamboa-Delgado et al, 2011;Hachero-Cruzado et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cloning and molecular ontogeny of digestive enzymes in fed and food-deprived developing gilthead seabream (Sparus aurata) larvae

Mata-Sotres

Martos-Sitcha

Astola

et al. 2016

Comparative Biochemistry and Physiology Part B: Biochemistry an

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We have determined the expression pattern of key pancreatic enzymes precursors (trypsinogen, try; chymotrypsinogen, ctrb; phospholipase A2, pla2; bile salt-activated lipase, cel; and α-amylase, amy2a) during the larval stage of gilthead seabream (Sparus aurata) up to 60days after hatching (dph). Previously, complete sequences of try, cel, and amy2a were cloned and phylogenetically analyzed. One new isoform was found for cel transcript (cel1b). Expression of all enzyme precursors was detected before the mouth opening. Expression of try and ctrb increased during the first days of development and then maintained high values with some fluctuations during the whole larval stage. The prolipases pla2 and cel1b increased from first-feeding with irregular fluctuation until the end of the experiment. Contrarily, cel1a maintained low expression values during most of the larval stage increasing at the end of the period. Nevertheless, cel1a expression was negligible as compared with cel1b. The expression of amy2a sharply increased during the first week followed by a gradual decrease. In addition, a food-deprivation experiment was performed to find the differences in relation to presence/absence of gut content after the opening of the mouth. The food-deprived larvae died at 10dph. The expression levels of all digestive enzymes increased up to 7dph, declining sharply afterwards. This expression pattern up to 7dph was the same observed in fed larvae, confirming the genetic programming during the early development. Main digestive enzymes in gilthead seabream larvae exhibited the same expression profiles than other marine fish with carnivorous preferences in their juvenile stages.

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Characterization of the genomic responses in early Senegalese sole larvae fed diets with different dietary triacylglycerol and total lipids levels

Cited by 28 publications

References 84 publications

Expression analyses of digestive enzymes during early development and in adults of the chame fishDormitator latifrons

Expression analyses of digestive enzymes during early development and in adults of the chame fishDormitator latifrons

Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta)

Cloning and molecular ontogeny of digestive enzymes in fed and food-deprived developing gilthead seabream (Sparus aurata) larvae

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