Characterization of the human E2F4 promoter region and its response to 12-O-tetradecanoylphorbol-13-acetate

Hamada, Hiroshi; Goto, Yuta; Arakawa, Jun; Murayama, Erisa; Ogawa, Yasuhiro; Konno, Midori; Oyama, Takahiro; Sato, Akira; Tanuma, Sei Ichi; Uchiumi, Fumiaki

doi:10.1093/jb/mvz047

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…In addition, RELB is required for TNF-induced differentiation of bone marrow cells into M1 macrophages in mice ( 33 ). Our previous studies indicated that the duplicated GGAA-motif is responsible for the activation of the E2F4 and ZNFX1 genes during TPA-induced macrophage-like differentiation of HL-60 cells ( 34 , 35 ). In HeLa S3 cells, the Sp1/PU.1 ratio is notably increased following the Rsv treatment ( 21 ).…”

Section: Discussionmentioning

confidence: 99%

Induction of the human CDC45 gene promoter activity by natural compound trans‑resveratrol

Arakawa,

Kondoh,

Matsushita

et al. 2024

Mol Med Rep

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GGAA motifs in the human TP53 and HELB gene promoters play a part in responding to trans -resveratrol (Rsv) in HeLa S3 cells. This sequence is also present in the 5′-upstream region of the human CDC45 gene, which encodes a component of CMG DNA helicase protein complex. The cells were treated with Rsv (20 µM), then transcripts and the translated protein were analyzed by quantitative RT-PCR and western blotting, respectively. The results showed that the CDC45 gene and protein expression levels were induced after the treatment. To examine whether they were due to the activation of transcription, a 5′-upstream 556-bp of the CDC45 gene was cloned and inserted into a multi-cloning site of the Luciferase (Luc) expression vector. In the present study, various deletion/point mutation-introduced Luc expression plasmids were constructed and they were used for the transient transfection assay. The results showed that the GGAA motif, which is included in a putative RELB protein recognizing sequence, plays a part in the promoter activity with response to Rsv in HeLa S3 cells.

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Section: Discussionmentioning

confidence: 99%

Induction of the human CDC45 gene promoter activity by natural compound trans‑resveratrol

Arakawa,

Kondoh,

Matsushita

et al. 2024

Mol Med Rep

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Transcription factor E2F4 is a positive regulator of milk biosynthesis and proliferation of bovine mammary epithelial cells

Zhen

Zhang

Yuan

et al. 2019

Cell Biology International

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The transcription factor E2F4 is a key determinant of cell differentiation and cell‐cycle progression, but its function and regulatory mechanism are not completely understood. Here, we report that E2F4 acts as a positive regulator of the biosynthesis of milk components and proliferation of bovine mammary epithelial cells (BMECs). Overexpression of E2F4 in BMECs resulted in the upregulation of β‐casein, triglyceride, and lactose levels and increased cell proliferation, whereas E2F4 knockdown by small interfering RNA had the opposite effects. We further detected that overexpression of E2F4 significantly increased the messenger RNA expression of mTOR, SREBP‐1c, and Cyclin D1, and increased protein levels of SREBP‐1c, and Cyclin D1, and the ratio of p‐mTOR/mTOR, whereas E2F4 knockdown had the opposite effects. E2F4 was almost entirely located in the nucleus, and we further identified, via ChIP‐qPCR analysis, that mTOR, SREBP‐1c, and Cyclin D1 were E2F4 target genes, and exogenous administration of methionine, leucine, β‐estradiol, and prolactin markedly increased the protein levels of E2F4 and its binding to the promoters of these three genes. In summary, our data reveal that E2F4 responds to extracellular stimuli and regulates the expression of mTOR, SREBP‐1c, and Cyclin D1 for milk biosynthesis and proliferation of BMECs.

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Characterization of the human zinc finger nfx‑1‑type containing 1 encoding ZNFX1 gene and its response to 12‑O‑tetradecanoyl‑13‑acetate in HL‑60 cells

et al. 2019

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Human promyelocytic HL-60 cells can be differentiated into macrophage-like cells by treatment with 12-O-tetra decanoylphorbol-13-acetate (TPA). Certain 5' upstream regions of the zinc finger protein (ZNF)-encoding genes contain duplicated GGAA motifs, which are frequently found in the TPA-responding gene promoter regions. To examine transcriptional responses to TPA, 5'flanking regions of human zinc finger CCCH-type containing, antiviral, ZNF252, ZNF343, ZNF555, ZNF782 and zinc finger nfx-1-type containing 1 (ZNFX1) genes were isolated by polymerase chain reaction (PCR) and ligated into a multiple-cloning site of the pGL4.10[luc2] vector. Transient transfection and a luciferase assay revealed that the ZNFX1 promoter most prominently responded to the TPA treatment. Deletion and point mutation experiments indicated that the duplicated GGAA motif in the 100-bp region positively responded to TPA. In addition, reverse transcription-quantitative PCR and western blotting showed that the mRNA and protein of ZNFX1 accumulate during the differentiation of HL-60 cells. These results indicated that expression of the TPA-inducible ZNFX1 gene, which belongs to the group of interferon-responsive genes, is regulated by the cis-action of the duplicated GGAA motif.

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Characterization of the human E2F4 promoter region and its response to 12-O-tetradecanoylphorbol-13-acetate

Cited by 3 publications

References 41 publications

Induction of the human CDC45 gene promoter activity by natural compound trans‑resveratrol

Induction of the human CDC45 gene promoter activity by natural compound trans‑resveratrol

Transcription factor E2F4 is a positive regulator of milk biosynthesis and proliferation of bovine mammary epithelial cells

Characterization of the human zinc finger nfx‑1‑type containing 1 encoding ZNFX1 gene and its response to 12‑O‑tetradecanoyl‑13‑acetate in HL‑60 cells

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