Characterization of the Leukocyte- and Platelet-Rich Fibrin Block: Release of Growth Factors, Cellular Content, and Structure

Castro, Ana Belén Salamanca; Cortellini, Simone; Temmerman, Andy; Li, Xin; Pinto, Nelson; Teughels, Wim; Quirynen, Marc

doi:10.11607/jomi.7275

Cited by 67 publications

(71 citation statements)

References 37 publications

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“…It has to be underlined, however, that those authors did not analyze the secretion of PDGF-BB, as we did. In our experimental conditions, after PDGF-BB, the most expressed factor was TGF-β, in accordance with Castro et al [31].…”

Section: Discussionsupporting

confidence: 92%

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Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Serafini

Lopreiato

Lollobrigida

et al. 2020

JCM

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Liquid fibrinogen is an injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by blood centrifugation. The aim of this study was to analyze the release of different growth factors in the liquid fibrinogen at different times and to assess possible correlations between growth factors and cell counts. The concentration of transforming growth factor beta 1 (TGF-β1), platelet-derived growth factor-AB (PDGF-AB), platelet-derived growth factor-BB (PDGF-BB), bone morphogenetic protein 2 (BMP-2), fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF) released by liquid fibrinogen were examined with ELISA at three time points (T0, time of collection; T7, 7 days; T14, 14 days). The cellular content of the liquid fibrinogen and whole blood was also calculated for each volunteer. A mean accumulation of platelets of almost 1.5-fold in liquid fibrinogen compared to whole blood samples was found. An increase of TGF-β1, PDGF-AB, FGF-2, and VEGF levels was detected at T7. At T14, the level of TGF-β1 returned to T0 level; PDGF-AB amount remained high; the levels of FGF-2 and VEGF decreased with respect to T7, but remained higher than the T0 levels; PDGF-BB was high at all time points; BMP-2 level was low and remained constant at all time points. TGF-β1, PDGF-AB, and PDGF-BB showed a correlation with platelet amount, whereas BMP-2, FGF-2, and VEGF showed a mild correlation with platelet amount. Due to the high concentration of platelets, liquid fibrinogen does contain important growth factors for the regeneration of both soft and hard tissue. The centrifugation protocol tested in this study provides a valid solution to stimulate wound healing in oral and periodontal surgery.

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Section: Discussionsupporting

confidence: 92%

“…A cumulative analysis for all donors at all time points revealed that PDGF-BB was the growth factor present at the highest concentration in the liquid fibrinogen, unlike another study in which TGF-β1 was the most released [31]. It has to be underlined, however, that those authors did not analyze the secretion of PDGF-BB, as we did.…”

Section: Discussioncontrasting

confidence: 68%

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Serafini

Lopreiato

Lollobrigida

et al. 2020

JCM

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“…Different groups have measured specific growth factors released by PRC, compared their enrichment in different types of PRC and measured their kinetics over time 18,19,22,23 . Nevertheless, the total secretome released by PRC has not been yet analysed in detail.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the secretome of leukocyte-platelet rich fibrin: towards a better understanding of its wound healing properties

Hermida-Nogueira

Barrachina

Morán

et al. 2020

Sci Rep

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Leukocyte-platelet rich fibrin (L-PRF) is extensively used in the dentistry field and other clinical scenarios due to its regeneration properties. the goal of the present study was to depict the L-pRf secretome and how it changes over time. We obtained L-PRF membranes and cultured them in DMEM. The secretome was collected at days 3, 7 and 21. The secretome at day 3 was analysed by LC-MS/ MS and differences over time were analysed by Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH). Overall, 705 proteins were identified in the secretome of L-PRF membranes after 3 days of culture, including growth factors (EGF, PDGFA) and proteins related to platelet and neutrophil degranulation. A total of 202 differentially secreted proteins were quantified by SWATH when comparing secretomes at days 3, 7 and 21. Most of them were enriched at day 3 such as MMP9, TSP1 and CO3. On the contrary, fibrinogen and CATS were found down-regulated at day 3. Growth factor and western blotting analysis corroborated the proteomic results. This is the most detailed proteome analysis of the L-PRF secretome to date. Proteins and growth factors identified, and their kinetics, provide novel information to further understand the wound healing properties of L-pRf. In the last two decades, the use of platelet-rich concentrates (PRC) has become very popular in different fields, especially in dentistry, due to their regeneration properties. Different scientists and companies have developed methods to obtained PRC with presence or absence of leukocytes but all of them with addition of anticoagulants to the tubes. In 2001, Choukroun et al. developed the second generation of PRC, Leukocyte platelet rich fibrin (L-PRF) 1 , which is obtained by blood centrifugation without anticoagulant in the tubes. Nowadays, L-PRF is the most commonly used PRC since it is easy to obtain and can be applied as a gel or compressed into a membrane depending on the application site. It has been mostly used in a dentistry field, especially for the treatment of osteonecrosis 2,3 , implant placement in bone-deficient sites 4 and sinus floor elevation 5. Additionally, L-PRF has started to be used in other clinical scenarios, for example for the treatment of chronic wounds, leg ulcers or diabetic foot 6 or for the treatment of ocular lesions 7. Wound healing is a complex process, which encompass different phases: haemostasis, inflammation, proliferation and remodelling. In each phase, different cell types take part in order to regenerate the tissue. Two types of blood cells, platelets and leukocytes, are among the cells that participate first. Growth factors, cytokines and other proteins released by these blood cells have chemoattractant properties that contribute to wound repair 8-10 .

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“…Numerosos son los usos clínicos descritos en la abultada literatura disponible actualmente para los concentrados plaquetarios, siendo quizás la reconstrucción de rebordes alveolares atróficos ( Fig. 1) como preparación previa a la instalación de implantes óseo integrados su principal indicación (Castro et al, 2019).…”

Section: Aplicaciones Clínicas De Los Concentra-dos Plaquetariosunclassified

L-PRF y Ciclo Celular. Revisión Narrativa

Correa-Aravena

Alister

Olate

et al. 2019

Int. J. Odontostomat.

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RESUMEN: Los concentrados plaquetarios han emergido como un potencial material regenerativo, utilizado de forma aislada o como andamiaje para otros materiales de injerto. Son extractos de sangre, obtenidos después de procesar una muestra de sangre completa, mediante centrifugación. El primer reporte data de 1970, con un CP utilizado como pegamento para mejorar cicatrización de heridas de piel. En 1998, se usaron en cirugía oral y maxilofacial. Desde entonces, se han desarrollado diferentes técnicas y una variedad de preparaciones. Entre ellas, cabe destacar el plasma rico en plaquetas, fibrina rica en plaquetas y leucocitos (L-PRF) y plasma rico en factores de crecimiento (PRGF). El desarrollo de estos biomateriales, se debe en parte, a la posibilidad de alterar la concentración de mediadores químicos liberados en una lesión que provoque la formación de un coágulo, que pueda madurar conforme transcurran las fases del proceso inflamatorio y concluya con la regeneración íntegra del tejido dañado. El objetivo de este manuscrito fue describir las principales vías de señalización intracelular que se activan en presencia del L-PRF en cirugía oral, y sus efectos en la regulación del ciclo celular. PALABRAS CLAVE: factores de crecimiento; receptores de citoquinas; agentes angiogénicos moduladores; periodoncia; endodoncia regenerativa; plaquetas ricas en fibrina; medicina regenerativa.

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Characterization of the Leukocyte- and Platelet-Rich Fibrin Block: Release of Growth Factors, Cellular Content, and Structure

Cited by 67 publications

References 37 publications

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

Deciphering the secretome of leukocyte-platelet rich fibrin: towards a better understanding of its wound healing properties

L-PRF y Ciclo Celular. Revisión Narrativa

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