Characterization of the respiration of 3T3 cells by laser-induced fluorescence during a cyclic heating process

Beuthan, J.; Dressler, Cathrin; Zabaryło, Urszula; Minet, Olaf

doi:10.1002/lapl.200910144

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…A significant amount of H + protons is released during the enzyme activity of the NADH dehydrogenase. H + protons are pumped into the mitochondrial intermembrane space by both the NADH hydrogenase and the cytochrome C complex, together with the cytochrome oxidase [49,50]. In this part when the spectrum of near 785 nm wavelength is absorbed by the ferrous heme a +2 3 in the c-oxidase cytochrome of the mitochondria [14], promotes rapid modifications in the cascade of intracellular chemical and physical reactions, leading to metabolic alterations [51].…”

Section: Discussionmentioning

confidence: 99%

In vitro effect of low-level laser on odontoblast-like cells

et al. 2010

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The aim of this study was to evaluate the metabolism of odontoblast-like MDPC-23 cells subjected to direct LLL irradiation. The cells were seeded (20,000 cells/well) in 24-well plates and incubated for 24 hours at 37• C. After this period, the culture medium (DMEM) was replaced by fresh DMEM supplemented with 2 or 5% (stress induction by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to laser doses of 2, 4, 10, 15 and 25 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780±3 nm, 40 mW). One control group (sham irradiation) was established for each experimental condition (laser dose x FBS supplementation). Three and 72 hours after the last irradiation, cells were analyzed with respect to metabolism, morphology, total protein expression and alkaline phosphatase (ALP) activity. Higher metabolism and total protein expression were observed 72 hours after the last irradiation at the doses of 15 and 25 J/cm 2 (Mann-Whitney; p<0.05). Higher ALP activity was obtained with 5% FBS when the cells were irradiated with doses of 2 and 10 J/cm 2 . For the dose of 25 J/cm 2 , the highest ALP activity was observed with 10% FBS. It was concluded that the LLLT parameters used in this study stimulated the metabolic activity of the MDPC-23 cells, especially at the doses of 15 and 25 J/cm 2 .

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Section: Discussionmentioning

confidence: 99%

In vitro effect of low-level laser on odontoblast-like cells

et al. 2010

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“…This fact cannot be described in a systemic context and needs to be analyzed as specific issues such as NAD(P)H coenzyme autofluorescence [28,39,40,44], intracellular calcium metabolism [15,16,41,42], and various subcellular structures [19] using analytical and numerical modeling [38,40,[43][44][45].…”

Section: Resultsmentioning

confidence: 99%

Thermally induced changes of optical and vital parameters in human cancer cells

et al. 2010

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Minimally invasive laser-induced thermotherapy (LITT) presents an alternative method to conventional tumor therapeutically interventions, such as surgery, chemotherapy, radiotherapy or nuclear medicine. Optical tissue characteristics of tumor cells and their heat-induced changes are essential issues for controlling LITT progressions. Therefore, it is indispensable to exactly know the absorption coefficient μ a , the scattering coefficient μ s and the anisotropy factor g as well as their changes under rising temperatures in order to simulate the treatment parameters successfully. Optical parameters of two different cancer model tissues -breast cancer cells species MX1 and colon cancer cells species CX1 -were measured in the spectral range 400 -1100 nm as well as in the temperature range 37 -60• C. The absorption coefficient of both cell species was low throughout the spectral range analyzed, while μ s of both species rose with increasing temperatures. The anisotropy factor g however dropped for both tissues with increasing temperatures. Light scatterings inside tissues proceeded continuously forward for all species tested. It was demonstrated that optical tissue properties undergo significant changes along with the vital status of the cells when the temperature increases. Screening of the metabolic activities of MX1 and CX1 cells after exposure to heat stress

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“…Thereby all subjective sources of error can be avoided during the diagnosis of the mac roscopic threshold. Furthermore the regeneration ability can be monitored for a longer period [21][22][23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Comparison of retina damage thresholds simulating the femtosecond-laser in situ keratomileusis (fs-LASIK) process with two laser systems in the CW- and fs-regime

Sander¹,

Minet

Zabaryło

et al. 2012

Laser Phys.

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The femtosecond laser in situ keratomileusis procedure affords the opportunity to correct ametropia by cutting transparent corneal tissue with ultra short laser pulses. Thereby the tissue cut is gener ated by a laser induced optical breakdown in the cornea with ultra short laser pulses in the near infrared range. Compared to standard procedures such as photorefractive keratectomy and laser in situ keratomileusis with the excimer laser, where the risk potential for the eye is low due to the complete absorption of ultraviolet irradiation from corneal tissue, only a certain amount of the pulse energy is deposited in the cornea during the fs LASIK process. The remaining energy propagates through the eye and interacts with the retina and the strong absorbing tissue layers behind. The objective of the presented study was to determine and compare the retina damage thresholds during the fs LASIK process simulated with two various laser systems in the CW and fs regime.

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Characterization of the respiration of 3T3 cells by laser-induced fluorescence during a cyclic heating process

Cited by 5 publications

References 27 publications

In vitro effect of low-level laser on odontoblast-like cells

In vitro effect of low-level laser on odontoblast-like cells

Thermally induced changes of optical and vital parameters in human cancer cells

Comparison of retina damage thresholds simulating the femtosecond-laser in situ keratomileusis (fs-LASIK) process with two laser systems in the CW- and fs-regime

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