Characterization of the viral genomes present in commercial batches of horse serum obtained by high-throughput sequencing

Paim, Willian Pinto; Weber, Matheus Nunes; Cibulski, Samuel Paulo; Silva, M.S. da; Puhl, D.E.; Budaszewski, R.F.; Varela, Ana Paula Muterle; Mayer, Fabiana Quoos; Canal, Cláudio Wageck

doi:10.1016/j.biologicals.2019.08.005

Cited by 11 publications

(15 citation statements)

References 33 publications

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“…Blood derivates are widely employed both for research purposes and in clinical practice. In research, many cell culture systems, vaccines, and the production of anti-sera depend on serum: the main product used in biotechnology is usually fetal bovine serum (FBS), but its frequent contamination with ruminant pestiviruses (family: Flaviviridae ) highlighted several issues that, to be overcome, resulted in attempts to adapt cell lines to equine sera [ 34 , 35 ]. Since 2012, many authors have searched for the presence of different viruses and Flaviviridae specimens in particular also in commercial equine serum products commonly used for the production of anti-sera, antitoxins, cell culture propagation, vaccines, and pregnant mare serum gonadotropin (PMSG) [ 1 , 11 , 34 , 35 , 36 , 37 ].…”

Section: Epidemiologymentioning

confidence: 99%

Equine Hepacivirus: A Systematic Review and a Meta-Analysis of Serological and Biomolecular Prevalence and a Phylogenetic Update

Pacchiarotti

Nardini

Scicluna

2022

Animals

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Viral hepatitis has recently assumed relevance for equine veterinary medicine since a variety of new viruses have been discovered. Equine Hepacivirus (EqHV) is an RNA virus belonging to the Flaviviridae family that can cause subclinical hepatitis in horses, occasionally evolving into a chronic disease. EqHV, to date, is considered the closest known relative of human HCV. EqHV has been reported worldwide therefore assessing its features is relevant, considering both the wide use of blood products and transfusions in veterinary therapies and its similitude to HCV. The present review resumes the actual knowledge on EqHV epidemiology, risk factors and immunology, together with potential diagnostics and good practices for prevention. Moreover, adhering to PRISMA guidelines for systematic reviews a meta-analysis of serological and biomolecular prevalence and an updated phylogenetic description is presented as a benchmark for further studies.

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Section: Epidemiologymentioning

confidence: 99%

Equine Hepacivirus: A Systematic Review and a Meta-Analysis of Serological and Biomolecular Prevalence and a Phylogenetic Update

Pacchiarotti

Nardini

Scicluna

2022

Animals

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“…Beside the above studies focused on Theiler’s disease viral metagenomics study of CSF from a horse with neurological signs identified another parvovirus named horse parvovirus-CSF [20]. Metagenomics studies of equine serum pools recently identified the four equine viruses listed above plus unexpectedly the porcine Suid betaherpesvirus 2 [21].…”

Section: Introductionmentioning

confidence: 99%

Viruses in Horses with Neurologic and Respiratory Diseases

Altan

Sabino‐Santos

et al. 2019

Viruses

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Metagenomics was used to identify viral sequences in the plasma and CSF (cerobrospinal fluid) of 13 horses with unexplained neurological signs and in the plasma and respiratory swabs of 14 horses with unexplained respiratory signs. Equine hepacivirus and two copiparvoviruses (horse parvovirus-CSF and a novel parvovirus) were detected in plasma from neurological cases. Plasma from horses with respiratory signs contained the same two copiparvoviruses plus equine pegivirus D and respiratory swabs contained equine herpes virus 2 and 5. Based on genetic distances the novel copiparvovirus qualified as a member of a new parvovirus species we named Eqcopivirus. These samples plus another 41 plasma samples from healthy horses were tested by real-time PCRs for multiple equine parvoviruses and hepacivirus. Over half the samples tested were positive for one to three viruses with eqcopivirus DNA detected in 20.5%, equine hepacivirus RNA and equine parvovirus-H DNA in 16% each, and horse parvovirus-CSF DNA in 12% of horses. Comparing viral prevalence in plasma none of the now three genetically characterized equine parvoviruses (all in the copiparvovirus genus) was significantly associated with neurological and respiratory signs in this limited sampling.

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“…In South America, the horse "hepatitis viruses" as designated by , have been studied since 2013, so far with the description of infections by equine hepacivirus (Hepacivirus A, EqHV) (A.S. Figueiredo et al, 2015), equine pegivirus (Pegivirus E, EPgV) and the Theiler's disease-associated virus (Pegivirus D, TDAV) in Brazil (Figueiredo et al, 2019). These viruses were also detected in Brazilian commercial horse serum (Paim et al, 2019), but the EqPV-H genome was not found in Brazilian or Chilean batches (Meister, Tegtmeyer, Postel, et al, 2019;Paim et al, 2019).…”

mentioning

confidence: 99%

Equine parvovirus‐hepatitis is detected in South America, Brazil

Moraes

Salgado

Godoi

et al. 2021

Transbounding Emerging Dis

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The equine parvovirus-hepatitis (EqPV-H), recently identified in association with serum hepatitis in horses (also known as Theiler's disease), has been so far described in horses from North America, Asia and Europe. There is no information regarding its circulation in South America. Our retrospective study (2013)(2014)(2015)(2016) screened by EqPV-H nested-PCR a total of 96 Brazilian horses grouped according to previous status of infection: Known to be positive for one or more horse "hepatitis viruses" (equine hepacivirus, equine pegivirus-EPgV and Theiler's disease-associated virus) and known to be negative. Serum biochemical parameters (aspartate aminotransferase, gammaglutamyl transferase and glutamate dehydrogenase) were evaluated in EqPV-H positive horses. Molecular characteristics of the isolates were analyzed by DNA sequencing and phylogenetic analysis. EqPV-H DNA was detected in 12.5% (12/96) of horses from 46.6% (7/15) of the farms evaluated. Similar results were obtained between coinfected group (12.3%, 7/57) and non-coinfected group (12.8%, 5/39). Coinfection with EPgV was the most frequent (5/7). Altered serum biochemical parameters suggested a subclinical hepatopathy in some animals (3/12), but the majority presented no clinical or laboratory signs of infection. Nucleotide identity was higher than 94% in comparison with previous isolates. In conclusion, we demonstrated, for the first time in South America, the circulation of EqPV-H. The Brazilian isolates presented a low genetic variability, thus corroborating previous evidence.

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Characterization of the viral genomes present in commercial batches of horse serum obtained by high-throughput sequencing

Cited by 11 publications

References 33 publications

Equine Hepacivirus: A Systematic Review and a Meta-Analysis of Serological and Biomolecular Prevalence and a Phylogenetic Update

Equine Hepacivirus: A Systematic Review and a Meta-Analysis of Serological and Biomolecular Prevalence and a Phylogenetic Update

Viruses in Horses with Neurologic and Respiratory Diseases

Equine parvovirus‐hepatitis is detected in South America, Brazil

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