Characterization of two PR genes from Fragaria chiloensis in response to Botrytis cinerea infection: A comparison with Fragaria x ananassa

González, Gloria; Fuentes, Lida; Moya-León, Marı́a Alejandra; Sandoval, C.; Herrera, Raúl

doi:10.1016/j.pmpp.2013.02.001

Cited by 32 publications

(25 citation statements)

References 45 publications

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“…In these wild accessions, B. cinerea grew much slower. Comparative studies of disease progression indicated that fruit from the cultivar Chandler developed lesions at 24 hpi, while fruit from an F. chiloensis ecotype developed symptoms at 72 hpi (González et al , ). Fruit were entirely covered with mould at 6 days post‐infection (dpi) for the cultivar Chandler and at 9 dpi for the F. chiloensis ecotype.…”

Section: Variation Of Quantitative Resistance To Botrytis Cinerea In mentioning

confidence: 99%

“…Another mechanism of resistance could be the presence of fungal inhibitors or the induction of PR proteins. FcPR5 and FcPR10 are highly induced in resistant F. chiloensis accessions when compared to commercial F. x ananassa cultivars (González et al , ). Based on sequence homology, FcPR5 probably possesses antifungal activity, and FcPR10 is likely a ribonuclease.…”

Section: Variation Of Quantitative Resistance To Botrytis Cinerea In mentioning

confidence: 99%

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Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea

Petrasch

Knapp

Kan

et al. 2019

Molecular Plant Pathology

314

172

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Summary The fungal pathogen Botrytis cinerea causes grey mould, a commercially damaging disease of strawberry. This pathogen affects fruit in the field, storage, transport and market. The presence of grey mould is the most common reason for fruit rejection by growers, shippers and consumers, leading to significant economic losses. Here, we review the biology and epidemiology of the pathogen, mechanisms of infection and the genetics of host plant resistance. The development of grey mould is affected by environmental and genetic factors; however, little is known about how B. cinerea and strawberry interact at the molecular level. Despite intensive efforts, breeding strawberry for resistance to grey mould has not been successful, and the mechanisms underlying tolerance to B. cinerea are poorly understood and under‐investigated. Current control strategies against grey mould include pre‐ and postharvest fungicides, yet they are generally ineffective and expensive. In this review, we examine available research on horticultural management, chemical and biological control of the pathogen in the field and postharvest storage, and discuss their relevance for integrative disease management. Additionally, we identify and propose approaches for increasing resistance to B. cinerea in strawberry by tapping into natural genetic variation and manipulating host factors via genetic engineering and genome editing.

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Section: Variation Of Quantitative Resistance To Botrytis Cinerea In mentioning

confidence: 99%

Section: Variation Of Quantitative Resistance To Botrytis Cinerea In mentioning

confidence: 99%

Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea

Petrasch

Knapp

Kan

et al. 2019

Molecular Plant Pathology

314

172

View full text Add to dashboard Cite

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“…In particular, PR10 genes seem to play a central role in the response of the Rosaceae. Studies of the Rosaceae model plant Fragaria infected by pathogens with different lifestyles, such as the necrotrophic fungus Botrytis cinerea (González et al 2013), the obligate biotrophic powdery mildew fungus Podosphaera aphanis (Jambagi and Dunwell 2015) and the hemibiotrophic oomycete Phytophthora cactorum (Toljamo et al 2016), showed a strong upregulation of PR10 genes or major allergens. Studies on apple (Malus) infected with Venturia inaequalis (apple scab) (Poupard et al 2003;Cova et al 2017) and treated with fungal elicitors (Pühringer et al 2000) also showed an upregulation of the PR10 genes.…”

Section: Common Response To Both Pathogensmentioning

confidence: 99%

“…The recognition of a pathogen by the plant cells leads to drastic changes in the transcriptome and activates a diverse set of immune responses, including the synthesis of secondary metabolites, cell wall modifications and the expression of pathogenesis-related (PR) genes (Slusarenko et al 2000). In addition to common immune responses in both, compatible and incompatible interactions, the responses to fungal infections may involve specific reactions depending on the life style of the infecting fungus (González et al 2013;Silvia Sebastiani et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation

et al. 2019

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Key message Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification for powdery mildew. Abstract Plant pathogenic fungi successfully colonize their hosts by manipulating the host defense mechanisms, which is accompanied by major transcriptome changes in the host. To characterize compatible plant pathogen interactions at early stages of infection by the obligate biotrophic fungus Podosphaera pannosa, which causes powdery mildew, and the hemibiotrophic fungus Diplocarpon rosae, which causes black spot, we analyzed changes in the leaf transcriptome after the inoculation of detached rose leaves with each pathogen. In addition, we analyzed differences in the transcriptomic changes inflicted by both pathogens as a first step to characterize specific infection strategies. Transcriptomic changes were analyzed using next-generation sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throughput qPCR. We identified a large number of differentially regulated genes. A common set of the differentially regulated genes comprised of pathogenesis-related (PR) genes, such as of PR10 homologs, chitinases and defense-related transcription factors, such as various WRKY genes, indicating a conserved but insufficient PTI [pathogen associated molecular pattern (PAMP) triggered immunity] reaction. Surprisingly, most of the differentially regulated genes were specific to the interactions with either P. pannosa or D. rosae. Specific regulation in response to D. rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes, such as paralogs of PR1 and PR5, and other factors of the salicylic acid signaling pathway. Differently, inoculation with P. pannosa leads in addition to the general pathogen response to a downregulation of genes related to photosynthesis and cell wall modification.Keywords Black spot · Powdery mildew · MACE analysis · High-throughput qPCR · WRKY genes · PR genes Enzo Neu and Helena Sophia Domes have contributed equally to this work. Electronic supplementary materialThe online version of this article (https ://doi.

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“…Additionally, the gene expression of PR proteins [chitinase (CHI), PR10 and β-glucanase] was detected earlier in a susceptible variety after 24 h of C. acutatum inoculation (Miles et al, 2011). Furthermore, two PR genes (PR5 and PR10) were found to be over-expressed in Fragaria chiloensis and Fragaria x ananassa after B. cinerea infection and showed different expression profiles depending on the tissue analyzed (González et al, 2013). Vilanova et al (2014b) showed that P. expansum inoculation in apples induced a greater number of PR proteins [two CHIs, endoglucanase (EGL), pathogenesis-related thaumatin (TAU), and the defensin-like protein (DEFL)], than inoculation with P. digitatum.…”

Section: Unravelling Fruit Defense Mechanismsmentioning

confidence: 99%

Molecular aspects in pathogen-fruit interactions: Virulence and resistance

Tian

Torres

Ballester

et al. 2016

Postharvest Biology and Technology

159

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Fruit losses during postharvest storage and handling due to pathogen infections are one of the major problems in the global food chain supply. The application of chemical fungicides to control diseases is currently limited by legislation in some countries and also raises concerns about food and environmental safety. Exploring molecular aspects of pathogen-fruit interactions therefore has biological and economic significance as a means to help develop rational alternatives for disease control. In this review we present the current knowledge of molecular aspects in pathogen-fruit interactions, addressing the following topics: the application of new "omics" technologies for studying these interactions; the molecular mechanisms of fungal pathogen attack; the regulation of virulence by exogenous factors; and, finally, fruit defense mechanisms.

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Characterization of two PR genes from Fragaria chiloensis in response to Botrytis cinerea infection: A comparison with Fragaria x ananassa

Cited by 32 publications

References 45 publications

Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea

Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea

Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation

Molecular aspects in pathogen-fruit interactions: Virulence and resistance

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