Characterizing components of the Saw Palmetto Berry Extract (SPBE) on prostate cancer cell growth and traction

Scholtysek, Carina; Krukiewicz, Aleksandra A.; Alonso, José L.; Sharma, Karan; Sharma, Pal C.; Goldmann, Wolfgang H.

doi:10.1016/j.bbrc.2008.11.114

Cited by 39 publications

(25 citation statements)

References 19 publications

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“…p53 increased after treatment with this extract, whereas p27 and p21 decreased. This report justifies the administration of the entire phytocomplex to ensure a proper pharmacological efficacy not limiting the interest only to free fatty acids [36];…”

Section: Discussionmentioning

confidence: 93%

Modern extraction techniques and their impact on the pharmacological profile of Serenoa repens extracts for the treatment of lower urinary tract symptoms

et al. 2014

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BackgroundBioactive compounds from plants (i.e., Serenoa repens) are often used in medicine in the treatment of several pathologies, among which benign prostatic hyperplasia (BPH) associated to lower urinary tract symptoms (LUTS).DiscussionThere are different techniques of extraction, also used in combination, with the aim of enhancing the amount of the target molecules, gaining time and reducing waste of solvents. However, the qualitative and quantitative composition of the bioactives depends on the extractive process, and so the brands of the recovered products from the same plant are different in terms of clinical efficacy (no product interchangeability among different commercial brands).SummaryIn this review, we report on several and recent extraction techniques and their impact on the composition/biological activity of S. repens-based available products.

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Section: Discussionmentioning

confidence: 93%

Modern extraction techniques and their impact on the pharmacological profile of Serenoa repens extracts for the treatment of lower urinary tract symptoms

et al. 2014

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“…7,15,28 As shown in Figure 3, E-Syt1 bound specifically to biotinylated β-sitosterol at 600 nM, just as in macrophage lysates. In contrast, 17β-HSD4 bound more strongly to biotinylated cholesterol than biotinylated β-sitosterol in both prostate cancer cell lines (Figure 3), which is opposite to what we observed in macrophage lysates.…”

mentioning

confidence: 69%

“…5,17 In support of this hypothesis, β-sitosterol exhibits growth inhibitory and cytotoxic effects against a range of cancer cell lines. 7,18–20 However, the precise molecular mechanisms underlying its health promoting effects remain largely uncharacterized. To understand the molecular mechanism(s) by which β-sitosterol exerts its many beneficial health effects, we performed affinity chromatography using biotinylated β-sitosterol to identify its protein targets.…”

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confidence: 99%

Identification and characterization of β-sitosterol target proteins

Lomenick

Shi

Huang

et al. 2015

Bioorganic & Medicinal Chemistry Letters

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β-Sitosterol is the most abundant plant sterol in the human diet. It is also the major component of several traditional medicines, including saw palmetto and devil’s claw. Although β-sitosterol is effective against enlarged prostate in human clinical trials and has anti-cancer and anti-inflammatory activities, the mechanisms of action are poorly understood. Here, we report the identification of two new binding proteins for β-sitosterol that may underlie its beneficial effects.

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“…Stigmasterol (4b) decreased tumor volume and cell viability, increasing the life span of Ehrlich ascites carcinoma (EAC)-bearing mice (Ghosh, 2011). It was reported that 4a and 4b exhibited anti-proliferative activities against human prostate cancer cells (DU-145) by increasing p53 protein expression and inhibiting carcinoma development by decreasing p21 and p27 protein expression (Scholtysek et al, 2009). Other studies reported that 4b showed cytostatic activity against HEp-2 and McCoy cells (Gómez et al, 2001), significantly inhibited tumor promotion in mouse skin two-stage carcinogenesis experiments (Kasahara et al, 1994), and demonstrated antimutagenic (Lim et al, 2005), and anti-oxidant (Panda et al, 2009) properties.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob.

Reyes¹,

Oyong²,

Ebajo³

et al. 2015

J App Pharm Sci

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The triterpenes, squalene (1), friedelin (2) and a mixture of ursolic acid (3a) and oleanolic acid (3b) in a 2:3 ratio, and a mixture of β-sitosterol (4a) and stigmasterol (4b) in a 2:1 ratio, obtained from the dichloromethane extract of Pipturus arborescens (Link) C.B. Rob., were evaluated for their anti-proliferative activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast-neonatal (HDFn) using the in vitro PrestoBlue ® cell viability assay. The HCT-116 cell line was most susceptible to the compounds and mixtures tested. Triterpene 1 was most cytotoxic against HCT-116 and MCF-7 with IC50 values of 4.21 and 5.92 μg/mL, respectively. Triterpene 2 and the mixture of 3a and 3b were highly anti-proliferative against HCT-116 cells (IC50 of 1.22 and 1.66 μg/mL, respectively) and moderately inhibitory against MCF-7 cells (IC50 of 16.51 and 23.97 μg/mL, respectively). The mixture of 4a and 4b exhibited high cytotoxicity against HCT-116 cells (IC50 of 1.14 μg/mL). Compounds 1-4b showed the least activity against HT-29 cells (IC50 of 11.97 to 52.52 μg/mL). Cytotoxic effect was not observed against HDFn cells (>100 μg/mL). Comparing the effects of 1-4b on the two colon cancer cell lines, the IC50 values of 1-4b against HCT-116 were lower than those of HT-29.

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Characterizing components of the Saw Palmetto Berry Extract (SPBE) on prostate cancer cell growth and traction

Cited by 39 publications

References 19 publications

Modern extraction techniques and their impact on the pharmacological profile of Serenoa repens extracts for the treatment of lower urinary tract symptoms

Modern extraction techniques and their impact on the pharmacological profile of Serenoa repens extracts for the treatment of lower urinary tract symptoms

Identification and characterization of β-sitosterol target proteins

Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob.

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