1986
DOI: 10.1042/bj2390301
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Charged anaesthetics alter LM-fibroblast plasma-membrane enzymes by selective fluidization of inner or outer membrane leaflets

Abstract: The functional consequences of the differences in lipid composition and structure between the two leaflets of the plasma membrane were investigated. Fluorescence of 1,6-diphenylhexa-1,3,5-triene(DPH), quenching, and differential polarized phase fluorimetry demonstrated selective fluidization by local anaesthetics of individual leaflets in isolated LM-cell plasma membranes. As measured by decreased limiting anisotropy of DPH fluorescence, cationic (prilocaine) and anionic (phenobarbital and pentobarbital) amphi… Show more

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Cited by 39 publications
(15 citation statements)
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“…Decreasing the plasma membrane cholesterol-to-phospholipid ratio significantly increases membrane fluidity and inhibits Na ϩ -K ϩ -ATPase activity (34). Agents that fluidize plasma membranes significantly inhibit Na ϩ -K ϩ -ATPase activity (89,90). Fatty acid-induced cytotoxicity is observed in L cells when membrane fluidity is outside an optimal range (75).…”
Section: Discussionmentioning
confidence: 96%
“…Decreasing the plasma membrane cholesterol-to-phospholipid ratio significantly increases membrane fluidity and inhibits Na ϩ -K ϩ -ATPase activity (34). Agents that fluidize plasma membranes significantly inhibit Na ϩ -K ϩ -ATPase activity (89,90). Fatty acid-induced cytotoxicity is observed in L cells when membrane fluidity is outside an optimal range (75).…”
Section: Discussionmentioning
confidence: 96%
“…Secondly, the plasmalemma also contains lateral domains (lipid rafts/caveolae as well as non-raft domains) that differ in their lipid composition and protein distribution (for reviews, see Pike, 2003;Pike et al, 2002;Schroeder et al, 2005). For example, the Na + ,K + -ATPase appears to be distributed primarily in the non-raft domains of the plasmalemma, at least in some cells (Atshaves et al, 2003;Gallegos et al, 2006) (Schroeder and Sweet, 1988;Sweet and Schroeder, 1986a;Sweet and Schroeder, 1986b;Sweet and Schroeder, 1988a). Lipid bilayers with a high content of cis-polyunsaturated fatty acid chains are both thinner and more flexible than bilayers with predominantly monounsaturated fatty acids (Rawicz et al, 2000).…”
Section: Discussionmentioning
confidence: 99%
“…Na + /K + -ATPase molecular activity has also been shown to correlate more strongly with the lateral pressure of membrane lipid mixtures than with the composition of any individual fatty acid in the membrane (Wu et al, 2001). Na + /K + -ATPase appears to be distributed primarily in non-raft domains of the cell membrane (Atshaves et al, 2003;Gallegos et al, 2006) and is influenced by the transbilayer fluidity gradient (Schroeder et al, 2005;Schroeder and Sweet, 1988;Sweet and Schroeder, 1986a;Sweet and Schroeder, 1986b;Sweet and Schroeder, 1988). This suggests that differences in molecular activity are likely to be influenced by the physical properties of the bulk phase membrane lipids created by the acyl composition of the phospholipids.…”
Section: Discussionmentioning
confidence: 99%