Differences in membrane acyl phospholipid composition between an endothermic mammal and an ectothermic reptile are not limited to any phospholipid class

Abstract

This study examined questions concerning differences in the acyl composition of membrane phospholipids that have been linked to the faster rates of metabolic processes in endotherms versus ectotherms. In liver, kidney, heart and brain of the ectothermic reptile, Trachydosaurus rugosus, and the endothermic mammal, Rattus norvegicus, previous findings of fewer unsaturates but a greater unsaturation index (UI) in membranes of the mammal versus those of the reptile were confirmed. Moreover, the study showed that the distribution of phospholipid head-group classes was similar in the same tissues of the reptile and mammal and that the differences in acyl composition were present in all phospholipid classes analysed, suggesting a role for the physical over

Diblock Copolymer Hydrophobicity Facilitates Efficient Gene Silencing and Cytocompatible Nanoparticle-Mediated siRNA Delivery to Musculoskeletal Cell Types

“…Differences in uptake mechanisms are unknown, but could be partly attributed to species and/or cell type differences in lipid bilayer composition. 54,55 Cationic drug delivery systems, such as PEI and Lipofectamine, are endocytosed after interaction with anionic surface receptors. 45,56–58 However, differences in endocytosis receptors have not been characterized in these cell types or across different species.…”

High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry

“…Importantly, this device produces similar lipid profiles as compared to syringe pump-driven capillary interfaces [40] and conventional nanoESI (not shown). We note that several studies have successfully applied the TriVersa NanoMate device for shotgun lipidomics analysis of lipid extracts [4,8,23,31,37,[40][41][42].…”

“…We also note that these GPL standards are detected in the same PIS spectrum (PIS m/z 269.25 C17:0) that allows the intensity profile of the standards to be used as a quality control of the analysis. The C17:0 containing lipid standards have successfully been applied in various quantitative lipidomic studies, either by shotgun lipidomics [4,8,42] or LC-based lipidomics [10,60]. An overview of the mode of analysis we use for detection of different lipid species and the respective internal standards are shown in Table 1.…”

In vivo MRS assessment of altered fatty acyl unsaturation in liver tumor formation of a TGFα/c-myc transgenic mouse model

“…More recently this effect has been characterized to occur under conditions of high total lipid concentration. At low concentrations, a linear correlation of ion intensity with the lipid concentration of each class is observed (25)(26)(27)(28). In this study, although we did not know a priori the absolute concentration of the lipid extracts, mass spectra of each lipid extract were initially acquired at a range of different dilutions to determine the dilution range at which linearity in the response of specific lipids was observed.…”