Chemical analyses of the cell wall of the murine leprosy bacillus

Kanetsuna, Fuminori; Imaeda, T; Blas, F San

doi:10.1128/jb.96.3.860-861.1968

Cited by 8 publications

(3 citation statements)

References 3 publications

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“…The chemical composition of cell walls of various mycobacteria (M. phlei, M. rhodochrous, M. tuberculosis, M. bovis, M. smegmatis, M. lepraemurium, Mycobacterium sp., and murine leprosy bacillus) has been studied qualitatively (2,34,76) and quantitatively (78,180,181,255). The cell walls always contained the amino acids Ala, Glu, m-Dpm and the monosaccharides arabinose and galactose.…”

Section: Gram-negative Bacteriamentioning

confidence: 99%

Peptidoglycan types of bacterial cell walls and their taxonomic implications.

Schleifer¹,

Kandler²

1972

Bacteriological Reviews

2,530

1,259

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Section: Gram-negative Bacteriamentioning

confidence: 99%

Peptidoglycan types of bacterial cell walls and their taxonomic implications.

Schleifer¹,

Kandler²

1972

Bacteriological Reviews

2,530

1,259

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“…These infected tissues were prepared in the National Hansen's Disease Center and supplied through the National Institutes of Health. d M. lepraemurium was isolated from spleens and mesenteric nodules of infected Swiss white mice as previously described (16). ' ATCC, American Type Culture Collection.…”

mentioning

confidence: 99%

DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation

Imaeda¹,

Kirchheimer²,

Barksdale³

1982

J Bacteriol

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DNA derived from Mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. The genome size and the guanineplus-cytosine content of M. leprae were 1.3 x 109 and 55.8%, respectively. Among selected strains of mycobacterial, nocardial, and corynebacterial species, Corynebacterium sp. 2628 LB, isolated from a human leprosy patient, showed the highest DNA homology with M. leprae. Of the DNAs derived from mycobacteria, those of M. tuberculosis and M. scrofulaceum showed a comparatively high reassociation with the DNA of M. leprae.

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“…Uyeda (18,19) claimed that, from the morphological features of this bacillus, it should not be classified as a mycobacterium, but rather should be placed in a new genus related to nocardia. However, results of recent works on the chemical analysis of the M. lepraemurium cell wall (7)(8)(9)14) suggest that its chemical composition qualitatively resembles that of the cell walls of mycobacteria (15). Also, Fukui and co-workers (12) have reported that the crude extracts of eight strains of M. lepraemurium contain a cross-reactive material whose antigenic determinants are partially in common with an alpha antigen of Mycobacterium avium.…”

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Cell Wall of Mycobacterium lepraemurium Strain Hawaii

et al. 1973

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The chemical properties of the cell wall of Mycobacterium lepraemurium strain Hawaii were investigated. Five subunits of the cell wall, arabinose mycolate, mycolic acids, tetrapeptide (Ala-Gln-diaminopimelic acid-Ala), disaccharide (N-acetylglucosaminyl.B-1,4-N-glycolylmuramic acid), and arabinogalactan, were obtained, and their chemical structures were identified.It is well known that Mycobacterium lepraemurium is the causative microorganism of murine leprosy, a chronic infection in the rat and the mouse. Its taxonomy has not yet been established. Uyeda (18,19) claimed that, from the morphological features of this bacillus, it should not be classified as a mycobacterium, but rather should be placed in a new genus related to nocardia. However, results of recent works on the chemical analysis of the M. lepraemurium cell wall (7-9, 14) suggest that its chemical composition qualitatively resembles that of the cell walls of mycobacteria (15). Also, Fukui and co-workers (12) have reported that the crude extracts of eight strains of M. lepraemurium contain a cross-reactive material whose antigenic determinants are partially in common with an alpha antigen of Mycobacterium avium. This finding also suggests the propriety of the serological typing of M. lepraemurium as a Mycobacterium.In this study, the chemical and immunological properties of the cell wall and polysaccharides of M. lepraemurium strain Hawaii have been examined in detail, and, based on these properties, it has been concluded that this microorganism is a Mycobacterium.Collection and fractionation of cells. A 0.2-ml portion of an emulsion of M. lepraemurium Hawaii leproma, diluted 1,000 times with Hanks solution, was inoculated subcutaneously into 400 4-week-old C3H mice. Six months later, the lepromas were excised and homogenized, and the M. lepraemurium Hawaii cells were collected by differential cen-' Present address: Rocky Mountain Laboratory, National

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Chemical analyses of the cell wall of the murine leprosy bacillus

Cited by 8 publications

References 3 publications

Peptidoglycan types of bacterial cell walls and their taxonomic implications.

Peptidoglycan types of bacterial cell walls and their taxonomic implications.

DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation

Cell Wall of Mycobacterium lepraemurium Strain Hawaii

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