Chemical and immunologic studies of low ragweed pollen extract

Studies on the allergens of ragweed pollen

“…Although precipitating antibodies to ragweed pollen extracts have never been found in sera of allergie individuals, ragweed pollen has been found to be antigenic in beth rabbits and guinea pigs, precipitating antibodies consistently being formed by beth these animals (23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)40). A large number of investigations have demonstrated that ragweed pollen contains several antigens (26,(28)(29)(30)(31)(32)37).…”

“…It is interesting to note that investigators who claimed to have prepared active carbohydrate fractions from WSR (27,50,78,79,82) were never able to eliminate the nitrogencontaining material from their active fractions without an accompanying loss of allergenic activity. In most cases, the nitrogencontaining material was regarded as an inactive contaminant.…”

Environmental Galactans Which React With IgA Myeloma T191B

“…Crude pollen extracts were prepared by extracting defatted pollen grains (Greer Laboratories) overnight at 4°C with Coca's solution (20% w/v). An arabinogalactan from Timothy pollen was isolated from a crude Timothy pollen extract by serial alcohol precipitation followed by chloroform treatment to remove protein (6). Further purification was carried out using Biogel P-200 columns, and the presence of arabinose and galactose was determined by thin-layer and gas chromatography (manuscript in preparation).…”