Chemical and photo-induced nuclease activity of a novel minor groove DNA binder Cu(II) complex

Abstract

A new type of copper(II) metal complex containing 1,10-phenanthroline (phen) and 8-(difluoromethoxy)-3,4-dihydro-2H-[1,3]thiazino[3,2-a]benzimidazole (dtb) ligands was prepared and characterized. The ds-DNA interaction of the complex was studied by UV-Vis spectrophotometry, competitive fluorometric titration with ethidium bromide (EB) and 4′,6-diamidino-2-phenylindole (DAPI), viscosity measurements and agarose gel electrophoresis. The results show that the complex can bind to ds-DNA in the minor groove by displacing DAPI molecules. DNA cleavage mechanism studies revealed that hydrogen peroxide radicals are responsible for DNA oxidative cleavage.

A binary copper(II) complex having stepped polymeric structure: Synthesis, characterization, DNA-binding and anti-fungal studies

“…The values of the DNA-binding constant of the complex determined through cyclic voltammetry and absorption spectroscopy were lower than the unsubstituted 2-phenylacetate copper(II) complex 10 and another minor groove binder of the copper(II) complex. 6 However, these values are comparable to some other copper(II) complexes [Cu(dipica)(diimine)] 2+ , [Cu(L-tyrosine)(5,6-dmp)] 2+ and [Cu(imda)(diimine)] complexes (dipica is di-2--picolylamine, 5,6-dmp is 5,6-dimethyl-1,10-phenanthroline and imda is iminodiacetate). [18][19][20] Viscommetry.…”

“…Owing to its bio-essentiality, its complexes are expected to be less toxic compared to the observed toxic side effects of the currently used platinum-based anticancer drugs. 6 Moreover, it has been found that one of the causes of the toxic behavior of platinum based drugs is their covalent and irreversible binding with cellular DNA of the target tissue. 7 In this context, any newly designed bioactive drug is desired to bind with DNA in a non-covalent binding mode.…”

Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes

“…We found that the addition of the silver(I) dipeptide complexes caused a small decrease in the initial viscosity, indicating the groove-binding mode of all three Ag(I) complexes (Figure 13). The analogous viscosimetric values were established for the [Cu(dtb)(phen)] 2+ complex, which binds into the minor groove of DNA [59].…”