Chemical characterization of essential oils from Drimys angustifolia miers (Winteraceae) and antibacterial activity of their major compounds

Abstract

Nos últimos anos, os óleos essenciais vêm sendo intensamente estudados como fonte natural de novos agentes antimicrobianos. Neste trabalho, os óleos essenciais das folhas e galhos de Drimys angustifolia do sul do Brasil foram obtidos por hidrodestilação e analisados por cromatografias gasosas com detector de ionização de chama (GC-FID) e com espectrômetro de massa (GC-MS). Os constituintes drimenol e biciclogermacreno foram isolados por cromatografia em coluna do óleo essencial dos galhos e folhas, respectivamente. Os óleos, os constituintes isolados e combinações destes foram testados contra bactérias Gram-(+) e Gram-(-). Os óleos essenciais foram mais ativos contra Bacillus cereus, com MIC (concentração inibitória mínima) de 125 e 250 µg mL -1 para os galhos e folhas, respectivamente, inibindo fortemente o crescimento bacteriano. Biciclogermacreno foi mais ativo que drimenol, fornecendo um valor de MIC de 167 µg mL -1 contra B. cereus. Não foi observado qualquer efeito sinérgico nas combinações testadas.Essential oils have been extensively studied in recent years as a natural source of new antimicrobial agents. In this work, essential oils of leaf and branch from Drimys angustifolia growing in Southern Brazil were obtained by hydrodistillation and analyzed by gas chromatographies with flame ionization detector (GC-FID) and with mass spectrometer (GC-MS). Drimenol and bicyclogermacrene were isolated by column chromatography from branch and leaf essential oils, respectively. Oils, isolated compounds and combinations of them were assayed against Gram-(+) and Gram-(-) bacteria. The oils showed to be more active against Bacillus cereus, with minimum inhibitory concentration (MIC) 125 and 250 µg mL -1 for branch and leaf oils, respectively, strongly inhibiting bacterial growth. Bicyclogermacrene was more active then drimenol, providing a MIC value of 167 µg mL -1 against B. cereus. Synergism was not observed in any of the combinations tested.

Chemical Characterization, Antioxidant Capacity and Antimicrobial Potential of Essential Oil from the Leaves of Baccharis oreophilaMalme

“…Essential oil obtained from B. parvidentada showed antimicrobial ability against S. aureus (MRSA BMB 9393) and C. albicans (ATCC 10231) with an MIC value of 1250 μg mL À 1 . The antimicrobial capacity of the B. oreophila essential oil found in this study can be attributed to its major components such as the oxygenated sesquiterpene spathulenol, which has antibacterial effect against S. Aureus [24,25] and also the minor ones such as hydrogenated sesquiterpenes δ-cadinene [26] and bicyclogermacrene [19] which have antibacterial effect. [7,23] In this sense, the essential oil obtained from B. oreophila leaves can be considered as a natural product with moderate capacity of inhibition against S. aureus and with weak inhibitory effect on C. tropicalis (ATCC 13803; Table 2).…”

Kazakh Ziziphora Species as Sources of Bioactive Substances

“…bungeana (0.3%) [201] Bicyclogermacrene (88) Z. clinopodioides (0.6%) [185] Low antibacterial effect (S. aureus, B. cereus, A. baumanii, E. coli, P. aeruginosa) [382] Germacrene D (87)…”

“…bungeana (0.3%) [201] Bicyclogermacrene (88) Z. clinopodioides (0.6%) [185] Low antibacterial effect (S. aureus, B. cereus, A. baumanii, E. coli, P. aeruginosa) [382] Z. clinopodioides subsp. rigida (1.1%) [21] Z. tenuior (0.13%) [94,179] Z. clinopodioides subsp.…”

“…rigida (1.1%) [21] Z. tenuior (0.13%) [94,179] Z. clinopodioides subsp. bungeana (0.3%) [201] Bicyclogermacrene (88) Z. clinopodioides (0.6%) [185] Low antibacterial effect (S. aureus, B. cereus, A. baumanii, E. coli, P. aeruginosa) [382] Molecules 2016, 21, 826 25 of 53…”

Antifreeze proteins in naturally cold acclimated leaves of Drimys angustifolia, Senecio icoglossus, and Eucalyptus ssp.

“…Three species grow wild in Brazil, namely, Drimys angustifolia Miers, D. brasiliensis Miers and D. roraimensis, the first two of which occur in southern Brazil. The D. angustifolia species is popularly known as "casca d'anta" or "cataia", and occurs more restrictively at high altitudes (SANTOS et al, 2013). The Senecio icoglossus DC.…”