Nos últimos anos, os óleos essenciais vêm sendo intensamente estudados como fonte natural de novos agentes antimicrobianos. Neste trabalho, os óleos essenciais das folhas e galhos de Drimys angustifolia do sul do Brasil foram obtidos por hidrodestilação e analisados por cromatografias gasosas com detector de ionização de chama (GC-FID) e com espectrômetro de massa (GC-MS). Os constituintes drimenol e biciclogermacreno foram isolados por cromatografia em coluna do óleo essencial dos galhos e folhas, respectivamente. Os óleos, os constituintes isolados e combinações destes foram testados contra bactérias Gram-(+) e Gram-(-). Os óleos essenciais foram mais ativos contra Bacillus cereus, com MIC (concentração inibitória mínima) de 125 e 250 µg mL -1 para os galhos e folhas, respectivamente, inibindo fortemente o crescimento bacteriano. Biciclogermacreno foi mais ativo que drimenol, fornecendo um valor de MIC de 167 µg mL -1 contra B. cereus. Não foi observado qualquer efeito sinérgico nas combinações testadas.Essential oils have been extensively studied in recent years as a natural source of new antimicrobial agents. In this work, essential oils of leaf and branch from Drimys angustifolia growing in Southern Brazil were obtained by hydrodistillation and analyzed by gas chromatographies with flame ionization detector (GC-FID) and with mass spectrometer (GC-MS). Drimenol and bicyclogermacrene were isolated by column chromatography from branch and leaf essential oils, respectively. Oils, isolated compounds and combinations of them were assayed against Gram-(+) and Gram-(-) bacteria. The oils showed to be more active against Bacillus cereus, with minimum inhibitory concentration (MIC) 125 and 250 µg mL -1 for branch and leaf oils, respectively, strongly inhibiting bacterial growth. Bicyclogermacrene was more active then drimenol, providing a MIC value of 167 µg mL -1 against B. cereus. Synergism was not observed in any of the combinations tested.

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Solid residues generated by the petroleum industry are often classified as hazardous, in part due to the lack of adequate analytical methods for their characterization, given their gluey and highly viscous nature. In this study, an alternative to the method described by the United States Environmental Protection Agency (US EPA) method 1311, replacing the zero headspace extraction (ZHE) approach with solid-phase microextraction followed by analysis via gas chromatographymass spectrometry was developed, for the determination of aromatic chlorinated and nonchlorinated volatile organic compounds. The method was validated according to national and international regulations, with a working range of 2.00 to 90.00 μg L -1 and limit of detection varying from 0.12 to 0.41 μg L -1 . Spiked matrices were analyzed and recovery values ranged from 79.7 to 98.4%. The analysis of real samples revealed levels below limit of quantification for chlorobenzene derivatives (2.00 μg L -1 ) and for the non-chlorinated aromatic compounds < 19.85 μg L -1, proving to be a viable alternative to overcome the difficulties encountered with filtration techniques.

Aiming to valorise the Atlantic Rainforest biodiversity in Santa Catarina, the chemical characterization of the essential oils (EOs) from leaves of Vernonanthura montevidensis (Spreng.) H. Rob. is described for the first time. Fresh leaves collected in the year 2014 and 2015, were submitted to hydrodistillation to give pale blue EOs in yields of 0.21 and 0.19%, respectively. The EOs were characterized by GC-MS and GC-FID quantitative method. The monoterpene β-pinene was the major constituent in both samples reaching a maximum of 26.3%. Besides β-pinene, the monoterpene α-pinene and the sesquiterpene β-caryophyllene, were among the major constituents in both samples. By means of extracted ion chromatogram procedure, it was possible to detect chamazulene, which was associated to the pale blue colour of the essential oils. In the in vitro antimollicute assays, the essential oil was moderately active against Mycoplasma genitalium and M. pneumoniae with MIC values of 250 µg mL -1 .