Chemical Composition, an Antioxidant, Cytotoxic and Microbiological Activity of the Essential Oil from the Leaves of Aeollanthus suaveolens Mart. ex Spreng

Abstract

Aeollanthus suaveolens species popularly known as catinga de mulata belongs to the Lamiaceae family. In the Amazon region, it is used in folk medicine for the treatment of gastritis, convulsions of epileptic origin, stomach pain and diarrhea in the form of tea and juice. Essential oils have analgesic, anti-inflammatory, and antimicrobial activity. This study evaluated the chemical composition of the A. suaveolens essential oil, and its cytotoxic, antimicrobial and antioxidant activity on Artemia salina Leach. The plant species was collected in Fazendinha district in the city of Macapa-AP. The essential oil obtained from the process was performed by hydrodistillation and identification of components by gas chromatography coupled with mass spectrometer. The antioxidant activity was evaluated by the kidnapping method of 2,2- diphenyl -1-picrilhidrazil radical, while the cytotoxic activity was assessed using saline A. and the microbiological activity was carried out by microdilution method with Escherichia coli, Salmonella sp. and Staphylococcus aureus bacteria. In a chromatographic analysis, the major constituents found in the essential oil of A. suaveolens were (E) -β-farnesene (37.615%), Linalool (33.375%), α-Santalene (3.255%) and linalyl acetate (3.222%). The results showed that the Escherichia coli and Salmonella sp. bacteria were more susceptible to MIC 50 mg.mL-1 when compared with the Staphylococcus aureus bacterium MIC 100 mg.mL-1. With respect to MBC concentration of 100 mg.mL-1 it was sufficient to inhibit the growth of E. coli. The essential oil did not show antioxidant activity, however, has a high cytotoxic activity against the A. salina, LC50 8.90 μg.mL-1.

Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae

“…The chemical compositions of the essential oils were determined by gas chromatography coupled to mass spectrometer (GC-MS) [ 17 ] on Shimadzu equipment, model CGMS-QP 2010 (Kyoto, Japan), on a DB-5HT column of the J & W Scientific brand, column 30 m, diameter 0.32 mm, film thickness 0.10 μm and nitrogen as carrier gas. The apparatus was operated under internal column pressure of 56.7 kPa, split ratio 1:20, gas flow in the column of 1.0 mL.min -1 (210 °C), injector temperature 220 °C, and the detector (CG-EM) of 240 °C.…”

Evaluation of the Larvicidal Potential of the Essential Oil Pogostemon cablin (Blanco) Benth in the Control of Aedes aegypti

“…The data in Table 3 show that the EO of P. cablin presents a LC 50 24.25 μg·mL −1 , p -value < 0.05 and coefficient of determination R 2 of 0.902. This value is above the toxicity standard of potassium dichromate with LC 50 of 12.60 μg·mL −1 , however, the EO of P. cablin has a high toxic action, because, according to Martins et al [16], pure substances extracted from plants are considered toxic when LC 50 < 100 μg·mL −1 , and nontoxic with LC 50 > 1000 μg·mL −1 .…”

“…The chemical composition of the EO was determined by gas chromatography coupled to mass spectrometer (GC-MS), using a model GCMS-QP 5050A, manufactured by Shimadzu company (Kyoto, Japan), under the following conditions: DB-5HT column of the brand J and W Scientific, with length of 30 m, diameter of 0.32 mm, film thickness 0.10 μm, and nitrogen as carrier gas, according to Martins et al [16].…”

Proteomics profiles reveal the potential roles of proteins involved in chicken macrophages stimulated by Lipopolysaccharide

“…Microorganisms compete with them for iron. It also has antimicrobial activity by detaching iron ions (Fe3+), which Gram-negative bacteria like Salmonella need (Martins et al 2016). Another is the complement C3, which is involved in immune defense and immune regulation in humans (Yan et al 2019).…”