Chemical composition, antioxidant and antibacterial activities of essential oil and methanol extract of Artemisia vulgaris and Gaultheria fragrantissima collected from Nepal

Pandey, Bishnu Prasad; Thapa, Rupak; Upreti, Anil

doi:10.1016/j.apjtm.2017.09.005

Cited by 59 publications

(51 citation statements)

References 26 publications

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“…Subsequently, A. vulgaris leaves extract has shown a reasonable potential of antioxidants activity with the percentage inhibition of 68.06% at 60 μg/ml concentration and ferric reducing power of 0.95 ± 0.05 at100 μg/ ml. The resulted antioxidant properties might be considered due to the high contents of flavonoid compound which act as a scavenging agents for different oxidizing species such as hydroxyl radical and super oxide anion (O2-•) [1]. Iqbal et al reported that the methanol extract of A. annua leaves showed the higher scavenging activities and reducing power comparatively with the other solvents.…”

Section: Discussionmentioning

confidence: 99%

“…Aromatics and Medicinal plants are one of the main significant sources of phytochemical constituent called secondary metabolites, which have been used widely in most of the pharmaceutical companies [1]. The World Health Organization (WHO) conducted a survey and reported that the total world′s populations of around 80% were depends on traditional knowledge of medicines, especially the medicinal plants for supporting their healthcare [2,3].…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves

2020

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Background Artemisia vulgaris commonly known as “mugwort” is a very important medicinal plant which is used widely for the treatment of various ailments traditionally. The present studies aimed to conduct preliminary phytochemical screening and evaluate antioxidants and analgesic activities of leaves extract. Results Phytochemical screening showed the presence of saponins, glycosides, flavanoids, protein, triterpenoids in leaves extract. In DPPH, percentage inhibition of the scavenging activity (68.06%) by the extract in comparison with ascorbic acid (93.53%) at 60 μg/ml while in reducing power assay, the result shown the reducing power increases with the increase of concentration as (0.13 ± 0.02), (0.27 ± 0.03), (0.42 ± 0.03), (0.62 ± 0.05), (0.79 ± 0.02), (0.95 ± 0.05) at concentration of 5, 10, 15, 30, 60 and 100 μg/ml respectively. The evaluation of analgesic activities revealed that at the dose of 200 and 400 mg/kg of the extract showed significantly inhibited the writhing response induced by acetic acid by 8.60% and 32.03% comparatively with indomethacin at a dose of 10 mg/kg b. wt. exhibited 56.87% inhibition. Whereas, in the tail immersion methods, at the dose of 400 mg/kg b. wt. was showed higher deflexion value as 3.40 ± 0.300, while 200 mg/kg b. wt. was resulted at 2.90 ± 0.200 value in dose dependent manner at the maximum time of the studies (60 min). Comparatively, the reference drug indomethacin at dose 10 mg/kg b. wt. has shown the highest deflexion value (5.134 ± 0.351). Conclusion This finding concludes that the methanolic leaves extract has a potent antioxidants and analgesic activities and it could be attributed to the presence of flavonoids, triterpenoids, saponin, glycosides and proteins. A further study is required to find out the novel bioactive compound of Artemisia vulgaris L. which can lead more effective in various biological activities.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves

2020

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“…Among the analysed metabolites, sabinene, β-myrcene, δ-3-carene, γ-terpinene, limonene, α-capaene, octanoic acid, and isocaryophillene were reported to have antimicrobial properties. [14][15][16][17][18][19] Identified metabolites reported to have antimicrobial and antioxidant properties are also tabulated in Table 5. Figure 4A).…”

Section: Gc-ms Analysis Of Eomentioning

confidence: 99%

TLC‐bioautography identification and GC‐MS analysis of antimicrobial and antioxidant active compounds in Musa × paradisiaca L. fruit pulp essential oil

Fahim

Ibrahim

Zahiruddin

et al. 2019

Phytochemical Analysis

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Introduction The absence of microbial growth and resistance to oxidative deterioration in fruits of Musa × paradisiaca L. (bananas) is an indication of the presence of antimicrobial and antioxidant metabolites. Objective In order to investigate the secondary metabolomic spectrum as well as the active antimicrobial and antioxidants present in essential oils (EOs) from fruits of different geographical areas of M. × paradisiaca, gas chromatography‐mass spectroscopy (GC‐MS) principal component data correlation analysis is complemented with antimicrobial assays and phytochemical and bioautographic antioxidant fingerprints with thin layer chromatography (TLC). Methodology An EO was obtained by steam distillation and subjected to GC‐MS and TLC for metabolomic profiling from fruit pulp. The antimicrobial potential was tested in both Escherichia coli as a gram negative and Bacillus subtilis as a gram positive microbe. Potential antioxidant metabolites were identified through TLC‐bioautography and GC‐MS analysis of active zones. Results A maximum of 0.56% v/w EO was isolated from fruit pulps of M. × paradisiaca. Minimum inhibitory concentrations (MICs) against B. subtillis and E. coli were 0.25 and 0.35 μg/mL, respectively. Thus, 56 metabolites were identified through GC‐MS. The major abundant antimicrobial metabolites found in EOs are α‐thujene, γ‐terpinene, α‐ and β‐pinene, sabinene, β‐myrcene, limonene, α‐capaene, caryophyllene and (Z,E)‐α farnesene. Aceteugenol, palmitic acid, stearic acid, palmitin, and stearin were identified as antioxidant metabolites. Principal component analysis of metabolite data reveals correlations and a clear separation based on metabolites obtained from various areas. Conclusion The data generated using metabolic profiling and cluster analysis helped to identify antimicrobial and antioxidant compounds in M. × paradisiaca.

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“…The bestdescribed property of almost every group of flavonoids is their ability to act as antioxidants. The flavonoids seem to be the most powerful compound to protect the body against reactive oxygen species [18].…”

Section: Extract and Fraction Of Daemonorops Dracomentioning

confidence: 99%

Antioxidant Activity of Daemonorops draco Resin

2019

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Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

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Chemical composition, antioxidant and antibacterial activities of essential oil and methanol extract of Artemisia vulgaris and Gaultheria fragrantissima collected from Nepal

Cited by 59 publications

References 26 publications

Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves

Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves

TLC‐bioautography identification and GC‐MS analysis of antimicrobial and antioxidant active compounds in Musa × paradisiaca L. fruit pulp essential oil

Antioxidant Activity of Daemonorops draco Resin

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