Antioxidant Activity of Daemonorops draco Resin

Abstract

Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

Antioxidant Activity, Sun Protection Activity, and Phytochemical Profile of Ethanolic Extracts of Daemonorops acehensis Resin and Its Phytosomes

“…This indicates that the increasing polarity of the solvent (more water) does not increase the compound's solubility from the resin to the solvent. However, previous studies have shown that the yield produced from an extraction with nonpolar solvents (n-hexane) produces a very small yield, while the highest yield was obtained in an extraction with ethyl acetate [11]. Other studies also showed that a higher ethanol composition increased the extract yield of propolis, which was dominated by resin [17].…”

“…In a previous report using D. draco resin, the antioxidant activity of the ethyl acetate extract was relatively high, with a half-maximal inhibitory concentration (IC 50 ) of 27.61 µg/mL [11]. Moreover, the methanol extract of D. draco resin from Sarolangun (Jambi, Indonesia) has antioxidant content with an IC 50 value of 117.63 ± 3.02 µg/mL [12].…”

Antioxidant and Antibacterial Activity of Selected Indonesian Honey against Bacteria of Acne

“…The solution was incubated for 30 minutes at room temperature. The absorbance of the solution was measured at a wavelength of 517 nm using a multi-well plate reader [ 19 ]. The positive control used was ascorbic acid.…”

Ektraksi getah jernang (Daemonorops draco) sistem basah dengan dua tahapan proses: perbedaan rendemen dan mutu [Extraction of dragon’s blood (Daemonorops draco) wet system by two-step process: effect of yield and quality]

“…Selain itu, getah jernang juga banyak digunakan untuk bahan baku pembuatan dupa. Di industri kesehatan, getah jernang banyak digunakan sebagai bahan obatobatan seperti obat disentri, diare, asma, obat untuk penyembuhan luka pada kulit (Jiang et al, 2017;; Namjoyan, Kiashi, Moosavi, Saffari, & Makhmalzadeh, 2016), obat untuk meningkatkan libido (aphrodisiac), bahan pasta gigi, anti bakteri, antifungal dan krem kulit antiageing (Gupta, Bleakley, & Gupta, 2007;; Jura-Morawiec, & Tulik, 2016) dan juga sebagai antioksidan (Purwanti, Wahyuni, & Batubara, 2019). Gambar 1.…”