Chemical Composition of Miscanthus sinensis var. purpurascens Flower Absolute and Its Beneficial Effects on Skin Wound Healing and Melanogenesis‐Related Cell Activities

Abstract

Miscanthus sinensis var. purpurascens (MSP, flame grass) is found in Korea, Japan, and China, and its biological activities include anti‐cancer, detoxifying, vasodilatory, antipyretic, and diuretic effects. However, no study has investigated the effects of MSP on skin‐related biological activities. In this study, we explored the effects of the absolute extracted from the MSP flowers (MSPFAb) on skin wound healing‐ and whitening‐related responses in keratinocytes or melanocytes. MSPFAb contained 6 components and induced the proliferation, migration, and syntheses of type I and IV collagens in keratinocytes. MSPFAb also increased the phosphorylations of serine/threonine‐specific protein kinase, p38 mitogen‐activated protein kinase, and extracellular signal‐regulated kinase1/2 in keratinocytes. In addition, treatment with MSPFAb decreased serum‐induced melanoma cell proliferation and inhibited tyrosinase activity and melanin contents in α‐MSH‐stimulated melanoma cells. Taken together, this study indicates MSPFAb may promote wound healing‐ and whitening‐associated activities in dermal cells, and suggests that it has potential use as a wound healing and skin whitening agent.

Chemical Composition of Salix koreensis Anderss Flower Absolute and Its Skin Wound Healing Activities In Vitro

“…Woodley et al reported that type I and type IV collagens increase keratinocyte migration [ 49 ]. Previous studies have shown many plant extracts enhance the syntheses and secretions of type I and/or IV collagens by HaCaT cells [ 25 , 30 , 50 ], which implies keratinocytes can synthesize and secrete collagen types I and IV. In the present study, SKAFAb increased collagen type I at concentrations of 150 μg/mL and 50 and IV syntheses 150 μg/mL in HaCaT cells.…”

Chemical Composition and Skin-Whitening Activities of Siegesbeckia glabrescens Makino Flower Absolute in Melanocytes

“…B16BL6 cell proliferation was assayed using a BrdU proliferation ELISA kit (Roche, Indianapolis, IN, USA) [24,51]. Briefly, 2 × 10 3 cells were seeded in each well of 96-well plates and cultured for 12 h. Different concentrations of SGMFAb in MEM containing 0.2% DMSO with or without 2% FBS were added to each well, followed by incubation for 36 h. The cells were labeled in BrdU-labeling solution (10 µM) for 12 h at 37 • C, and the culture medium was then removed.…”

“…Tyrosinase activity was analyzed based on the dopachrome method using L-DOPA as substrate, as reported previously [24,51]. In brief, B16BL6 cells (1 × 10 5 cells/dish) were cultured in 60 mm culture dishes for 12 h and then incubated in MEM (with 2% FBS) in the presence or absence of SGMFAb at different concentrations with or without α-MSH (200 nM) for 48 h at 37 • C. Cells were then lysed and centrifuged as in the melanin content assay described above.…”

“…Western blot analysis was carried out as in a previous report [24,51]. Cells were lysed using RIPA (radioimmunoprecipitation assay) buffer (Cell Signaling) and centrifuged (17,000× g, 15 min, 4 • C).…”