Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts

Gothai, Sivapragasam; Muniandy, Katyakyini; Zarin, Mazni Abu; Sean, Tan Woan; Kumar, Suresh; Munusamy, Murugan A.; Fakurazi, Sharida; Arulselvan, Palanisamy

doi:10.4103/pm.pm_368_16

Cited by 10 publications

(5 citation statements)

References 32 publications

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“…18 The M. oleifera leaves ethanol extract (MOLE), with its flavonoid compounds, is a novel candidate for skin wound healing with minimum side effects. 19 The gross wound size (Figure 1) significantly reduced after oral MOLE administration and was accompanied by a dose-dependent wound area reduction (Figure 2). This was in line with a previous study, where topical MOLE accelerated excisional wound healing in diabetic rats by reducing wound size, increasing wound contraction and tissue regeneration, downregulating inflammatory mediators, antibacterial activity, and regulating VEGF angiogenic markers.…”

Section: Discussionmentioning

confidence: 96%

Effect of Moringa Leave Ethanol Extract on Accelerating Wound Healing Process

Jaya,

Syamsunarno,

Sahiratmadja

et al. 2023

Glob Med Health Commun

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TGF-β and VEGF are vital in cell proliferation and regeneration, as evidenced in processes like wound healing. The leaves of Moringa oleifera Lam exhibit anti-inflammatory and cell regenerative properties that may facilitate the transition from the inflammatory to the proliferative phase, enhancing wound repair. This research sought to discern the potential of orally administered moringa leaf extract in augmenting systemic wound healing, focusing on TGF-β and VEGF serum as in vivo molecular markers. This research was conducted at the Animal Laboratory of the Faculty of Medicine, Universitas Padjadjaran, and the Laboratory of Molecular Genetics, Universitas Padjadjaran, from January to March 2022. We divided thirty Swiss Webster mice into two categories: healthy and wound-treated. Wounded mice received 100 mg/kgBW Na CMC as a negative control, 500 mg/kgBW zinc syrup as a positive control, and M. oleifera leaves ethanol extract (MOLE) in doses of 280 and 560 mg/kgBW orally from day 3 to day 6. Wound progression was documented and measured on days 0, -1, -3, and -6. Day-6 blood samples were obtained, and TGF-β and VEGF serum levels were gauged using ELISA. Results from day 6 revealed that wound coverage in the 280 and 560 mg/kgBW MOLE groups was 13.76±5% and 13.38±4%, respectively. These percentages notably surpassed that of the negative control group (p=0.005). However, the TGF-β and VEGF serum levels in the MOLE-treated groups did not differ significantly from the negative control (p=0.081 and p=0.149, respectively). Thus, the study concludes that while MOLE expedites wound closure, it does so without the systemic involvement of TGF-β and VEGF in vivo.

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Section: Discussionmentioning

confidence: 96%

Effect of Moringa Leave Ethanol Extract on Accelerating Wound Healing Process

Jaya,

Syamsunarno,

Sahiratmadja

et al. 2023

Glob Med Health Commun

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“…The scavenging activity increased in a concentration-dependent manner due to the scavenging capacity of the fraction and was comparable to quercetin. The IC50 value signifies the concentration required to scavenge 50% of the initial DPPH radicals (Gothai et al, 2017). The quercetin compound has the smallest IC50 value of 10.76 ppm with the highest antioxidant activity because this compound is a pure flavonoid compound with the maximum free radical scavenging.…”

Section: Discussionmentioning

confidence: 99%

“…Research showed that Moringa leaves contain quercetin, carotenoid, amino acids and alkaloids, as well as a combination of phenolic compounds (Gupta et al, 2015;Karthivashan et al, 2015). The antioxidant activity of ethanolic extract Moringa leaf is IC50 22.18 ppm Rizkayanti et al, (2017) while the ethyl acetate fraction was 18.21 ± 0.06 ppm (Gothai et al, 2017). The quercetin content of Moringa leaves has antioxidant activity, so it is necessary to study its effectiveness in regenerating skin cells topicall (Almeida et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

FORMULATION, EVALUATION OF PHYSICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACT AND ETHYL ACETATE FRACTION GEL OF Moringa oleifera LEAVES

Fatmawati¹,

Fauzi²,

Gunawan³

et al. 2022

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The leaves of Moringa (Moringa oleifera Lam) contain quercetin, a flavonoid that plays a role in the skin regeneration process with an antioxidant mechanism, can play a role in the healing process of skin wounds. The ethanol extract of Moringa leaves was fractionated using petroleum ether and ethyl acetate. The MLEE (Moringa leaf ethanolic extract) gel formula was made to vary the extract weights in the order of 2%, 4% and 6% (b/b), while EFML (Moringa leaf ethyl acetate fraction) gel preparations were made to vary the extract weights in the order of 1%, 2% and 4% (b/b). Evaluation of the physical properties of the gel preparations, including organoleptic observation, homogenity tests, pH measurements, spreadability tests, adhesion tests, and antioxidant activity using the DPPH method. The physical properties of all preparation fulfill the requirements. The test for antioxidant gel MLEE IC50: MLEE 1 144.72 ± 3.52 ppm; MLEE 2 138.15 ± 0.93 ppm; MLEE 3 136.59 ± 1.68 ppm when compared to extracts IC50 MLEE 23.14 ppm (very strong antioxidant activity). The EFML gel formulation (IC50: EFML 1 208.81 ± 4.09 ppm; EFML 2 193.22 ± 2.53 ppm; EFML 3 182.48 ± 2.11 ppm) gave the same results when compared to the thick fraction of Moringa leaves, which has moderate antioxidant activity. The formula gel MLEE 3 has moderate antioxidant activity, which is the best gel formulation based on the results of physical properties test.

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“…Phenolic compounds in the M. oleifera leaves EtOAc fraction have antioxidant activity that acts as hydrogen donors or reducing agents, which improves regeneration and organization of the new tissue in wound healing. 53 Moringa flowers and seeds also have antioxidant and anti-inflammatory activities due to their compounds such as ascorbic acid, carotenoids, tannins, alkaloids, glycosides, flavonoids and phenolic compounds kaempferol, quercetin, and the unique combination of fatty acids in the flower extract. 38,54 In an in vitro study, a combination of M. oleifera and Aloe vera containing quercetin to treat wounds, showed the highest anti-inflammatory effect and quercetin treatment with higher phenolic content was a modality with higher anti-inflammatory activity.…”

Section: Moringa Oleifera Compounds In Promoting Wound Healing Processmentioning

confidence: 99%

“…The M. oleifera ethyl acetate fraction supported the wound healing activity by promoting the proliferation and migration of fibroblast cells and neovascularization. 53 Moringa oleifera contains amino acids, fatty acids, vitamins, and trace elements that are all important in cellmediated immune responses. Fatty acids, vitamin D, and trace elements that not only facilitate the proliferation and maturation of neutrophils, but also the secretion of cytokines that enhance neutrophil migration and adhesion.…”

Section: Effect Of M Oleifera Extract On Chronic Woundsmentioning

confidence: 99%

Moringa oleifera Lam. to accelerate wound healing: a review

Jaya,

Syamsunarno,

Sahiratmadja

2023

JMedScie

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An injury to the skin that disrupts the soft tissue may form a wound. The healing process in response to injury is a dynamic and well-regulated process of cellular, humoral, and molecular mechanisms that consists of four partly overlapping phases: hemostasis, inflammation, proliferation, and remodeling. An impaired wound-healing process may cause a formation of an abnormal scar and chronic wounds, leading to a reduced life quality. Therefore, it needs an optimal prevention strategy. Many modalities have been claimed to accelerate wound healing. The trend of using natural products is increasing in most Southeast Asian countries due to their biodiversity. Nowadays, studies on natural compounds are increasing to accelerate wound healing. Moringa oleifera Lam. is a high-value plant that each part of it has a high nutritional value as well as a great range of medicinal uses, including anti-inflammatory, antimicrobial, antioxidant, and wound healing properties. In this review, we have explored the M. oleifera that are very rich in vitamins, minerals, fatty acids, and phytochemical compounds like quercetin, kaempferol, and vicenin-2, that play a role in the wound healing process. Moreover, these compounds may enhance the healing of wounds with pathological conditions such as diabetes, immunocompromised and persistent infection.

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Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts

Cited by 10 publications

References 32 publications

Effect of Moringa Leave Ethanol Extract on Accelerating Wound Healing Process

Effect of Moringa Leave Ethanol Extract on Accelerating Wound Healing Process

FORMULATION, EVALUATION OF PHYSICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACT AND ETHYL ACETATE FRACTION GEL OF Moringa oleifera LEAVES

Moringa oleifera Lam. to accelerate wound healing: a review

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