Chemical Composition of Tubers of Bletilla striata

Hu, Mingjuan; Jiang, Min; Zhang, Guo; Liu, Huayi; Yong, He; Wu, Zeng-Bao

doi:10.1007/s10600-019-02741-4

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…ESI-MS m/z 181.05 [M + H] + . Compound 5 was tentatively identified as caffeic acid (Hu et al, 2019).…”

Section: Isolation and Identificationmentioning

confidence: 99%

A study of the protease inhibitory activity component of SARS-CoV-2 3CL in Bletilla striata

Yang,

Zhang,

Liu

et al. 2024

Front. Nat. Prod.

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The protease Mpro (referred to as 3CLpro or 3CL protease) is a cysteine protease that is highly conserved in coronavirus and is indispensable for viral replication. Because there is no homologous protein for MPro in the human body, SARS-CoV-2 3CL protease is an ideal target against coronavirus. Bletilla striata (Reich. Bf.) is a well-known form of Traditional Chinese Medicine and can exert many pharmacological effects, including hemostasis, anti-microbial and anti-virus activities. Our preliminary screening showed that the n-butanol component of a methanol extract of B. striata exhibited potent inhibitory activity against SARS-CoV-2 3CL protease (58.82% at 200 μg/mL). In this study, we biologically evaluated ten isolated chemical compounds from B. striata and investigated the inhibitory activities of its constituents on SARS-CoV-2 3CL protease. Following bioactivity-guided fractionation, four bibenzyls (1, 4, 6 and 7), three phenols (5, 8 and 9), two anthraquinones (2 and 3) and one glucosyloxybenzyl 2-isobutylmalate (10) were isolated and evaluated for their ability to inhibit SARS-CoV-2 3CL protease by fluorescence resonance energy transfer (FRET) analysis. The binding mode between compounds and enzymes was investigated by molecular docking and Saturation Transfer Differences - nuclear magnetic resonance (STD-NMR). Moreover, Pleiobibenzynin B (7), Blestritin B, Gymconopin D (4), Physcion, 3′-O-methyl dioscin III (6), Gastrodin (8) and caffeic acid (5) all exhibited inhibitory activity against 3CL protease in vitro. The four bibenzyls (1, 4, 6 and 7) exhibited good inhibitory activity against SARS-CoV-2 3CL protease (7.37–39.86 μM). These findings identify potential new inhibitors against SARS-CoV-2 3CL protease, which offers promising lead compounds for the development of novel anti-SARS-CoV-2 drugs.

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“…ESI-MS m/z 181.05 [M + H] + . Compound 5 was tentatively identified as caffeic acid (Hu et al, 2019).…”

Section: Isolation and Identificationmentioning

confidence: 99%

A study of the protease inhibitory activity component of SARS-CoV-2 3CL in Bletilla striata

Yang,

Zhang,

Liu

et al. 2024

Front. Nat. Prod.

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“…[1,2] Additionally, active compounds such as militarine, ginsenoside II, and 6'''-acetylmilitaline have also been found in B. striata. [3] The pharmacological research showed that B. striata possessed various biological activities, such as antitumor, anti-inflammatory, neuroprotection, immunoenhancement, and antimicrobial. [4][5][6][7][8] B. striata polysaccharides (BSP) can promote wound healing.…”

Section: Introductionmentioning

confidence: 99%

“…Various active compounds have been isolated from B. striata , including polysaccharides, bibenzyl, anthocyanin glycosides, and saponins [1,2] . Additionally, active compounds such as militarine, ginsenoside II, and 6′′′‐acetylmilitaline have also been found in B. striata [3] . The pharmacological research showed that B. striata possessed various biological activities, such as antitumor, anti‐inflammatory, neuroprotection, immunoenhancement, and antimicrobial [4–8] .…”

Section: Introductionmentioning

confidence: 99%

Effect of Flowering Stages on the Content of Active Ingredients and Antioxidant Capability of Bletilla striata Flowers

Wang

Liu

Wang

et al. 2023

Chemistry & Biodiversity

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Bletilla striata (Thunb.) Reichb.f. is a perennial herb with abundant active ingredients. Previous research mainly focused on its tubers, however, the study on flowers, especially the variation of active ingredient contents at different flowering stages, was rarely seen. This study analyzed the total phenols, flavonoids, polysaccharides, anthocyanins, and cyanidin‐3‐O‐glucoside content of B. striata flowers which were in cultivated in Herb Garden of Zhejiang A&F University and collected in May, 2019, in order to investigate the changes in active ingredients and antioxidant capacity among different flowering stages (bud, initial, and full bloom). Changes in radical scavenging capability of DPPH (1,1‐Diphenyl‐2‐picrylhydrazyl radical), ABTS (2,2′‐azinobis(3‐ethylbenzthiazoline‐6‐sulphonate)), and hydroxy were analyzed. Significant differences in active ingredient content of flowers were detected among different flowering stages. The total phenolic content increased continuously during the entire flowering stage. The contents of total flavonoid, total polysaccharide, and cyanidin‐3‐O‐glucoside reached peaks at the initial blooming stage and then fell as the flowering process continued. The antioxidant activity in initial stage was the highest than in any other flowering stages. Therefore, we conclude that the initial blooming stage is the best harvesting stage of B. striata flowers. This study provides a robust basis for the harvest and utilization of B. striata flowers in food, medical, and cosmetic industries.

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The medicinal uses of the genus Bletilla in traditional Chinese medicine: A phytochemical and pharmacological review

Jiang

Wang

Jiang

et al. 2021

Journal of Ethnopharmacology

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Chemical Composition of Tubers of Bletilla striata

Cited by 4 publications

References 6 publications

A study of the protease inhibitory activity component of SARS-CoV-2 3CL in Bletilla striata

A study of the protease inhibitory activity component of SARS-CoV-2 3CL in Bletilla striata

Effect of Flowering Stages on the Content of Active Ingredients and Antioxidant Capability of Bletilla striata Flowers

The medicinal uses of the genus Bletilla in traditional Chinese medicine: A phytochemical and pharmacological review

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