Chemical constituents from the leaves ofPinus dalatensisFerré

Abstract

A phytochemical study of n-hexane and ethyl acetate extracts of Pinus dalatensis Ferré leaves led to the isolation of 11 compounds, including one caryolane sesquiterpenoid (1), five labdane diterpenoids (2, 3, 4, 5, 6), one serratane triterpenoid (7), one diacylated flavonoid glucoside (8), one stilbenoid (9) and two sterols (10, 11). The structural characterisation of the isolated compounds was elucidated by spectroscopic data and comparison with the literature report on the chemical constituents from Pinus dalatensis Ferré. Futhermore, three compounds 1, 4 and 6 were obtained for the first time from the genus Pinus. Besides, compounds (2, 3, 5, 8, 9) were also subjected to cytotoxicity effect on SK-LU-1, MCF-7 and Hep-G2 cell lines, but only compound 9 expressed activities with IC values of 141.22, 127.81 and 166.84 μM, respectively.

Chemical components and bioactivity evaluation of extracts from pear (Pyrus UssuriensisMaxim) fruit

“…Compound 2 (Sa et al, ): Daucosterol: White powder, 1 H‐NMR (CDCl 3 ): δ 5.35 (1H, m, H‐6), 4.43 (1H, m, H‐3), 4.25 (1H, d, J = 6.0 Hz, H‐1′). 13 C‐NMR (CDCl 3 ): δ 38.5 (C‐1), 29.1(C‐2), 77.2 (C‐3), 39.6 (C‐4), 140.1 (C‐5), 121.3 (C‐6), 33.2 (C‐7), 31.1 (C‐8), 50.1 (C‐9), 25.9 (C‐10), 21.3 (C‐11), 28.5 (C‐12), 42.2 (C‐13), 56.8 (C‐14), 24.5 (C‐15), 42.3 (C‐16), 55.9 (C‐17), 12.3 (C‐18), 18.7 (C‐19), 36.5 (C‐20), 18.6 (C‐21), 36.0 (C‐22), 37.1 (C‐23), 45.7 (C‐24), 29.3 (C‐25), 18.2 (C‐26), 20.3 (C‐27), 23.3 (C‐28), 11.5 (C‐29), 101.0 (C‐1′), 73.5 (C‐2′), 77.56 (C‐3′), 70.2 (C‐4′), 78.8 (C‐5′), 61.7 (C‐6′).…”

Antiproliferative activity of isolated compounds from Pinus dalatensis and Pinus krempfii on acute myeloid Leukemia cells

“…Pinus dalatensis and Pinus krempfii are endemic species in Vietnam, mainly distributed in Central Highlands Tay Nguyen, Vietnam. [1,3] Previous phytochemical studies of these plants lead to the isolation of diterpenes, sesquiterpenes, simple phenols, flavonoids, stilbenoids, lignans and other compounds. [1,2,3,4 ] Recent bioactivity experiments indicated that stilbenoid isolated from the leaves of P. dalatensis had cytotoxicity on SK-LU-1, MCF-7 and Hep-G2 cell lines.…”

“…[1,3] Previous phytochemical studies of these plants lead to the isolation of diterpenes, sesquiterpenes, simple phenols, flavonoids, stilbenoids, lignans and other compounds. [1,2,3,4 ] Recent bioactivity experiments indicated that stilbenoid isolated from the leaves of P. dalatensis had cytotoxicity on SK-LU-1, MCF-7 and Hep-G2 cell lines. [1] Additionally, all the isolated compounds from P. dalatensis wood showed a selective anti-proliferative activity on leukaemic cells.…”

“…[1,2,3,4 ] Recent bioactivity experiments indicated that stilbenoid isolated from the leaves of P. dalatensis had cytotoxicity on SK-LU-1, MCF-7 and Hep-G2 cell lines. [1] Additionally, all the isolated compounds from P. dalatensis wood showed a selective anti-proliferative activity on leukaemic cells. [2] On the other hand, favonoids from P. krempfii also had cytotoxic activity against KB and Hep-G2 cancer cell lines.…”

“…[3] Recently, compounds [8(17),13-ent-labdadien-15→16-lactone-19-oic acid (1), vanillic acid 4-β-D-glucopyranoside (2), chrysin (3) were isolated from P. dalatensis and galangin (4),cryptostrobin (5), strobopinin(6)] were isolated from P. krempfii (4)(5)(6). [1,3] However, there are no reports on the antiproliferative activity of these compounds on acute myeloid leukemia (ALM) cells. Hence, the present study describes the antiproliferative, apoptotic and cell cycle arrest effects on OCI-AML cancer cells of the compounds isolated from these two Pinus species growing in Vietnam.…”