Chemical fingerprint and quantitative analysis of Fructus Psoraleae by high‐performance liquid chromatography

Abstract

Fructus Psoraleae, a widely used traditional Chinese medicine, is well known as a health supplement ingredient. In our study, an improved and comprehensive HPLC fingerprint of Fructus Psoraleae was established. Two important new benzofuran glycosides, psoralenoside and isopsoralenoside, were identified as characteristic constituents for the first time. HPLC separation was performed on an RP-C8 column. The mobile phase was acetonitrile and 0.1% acetic acid solution with linear gradient change of acetonitrile from 10 to 82% in 40 min. The flow rate was 1.0 mL/min, and the detection wavelength was set at 310 nm. The HPLC chromatograms of twenty-six samples from different regions of China showed a similar pattern. Twelve peaks were selected as characteristic peaks and further identified as psoralenoside, isopsoralenoside, psoralen, isopsoralen, bavachromene, corylifolin, corylin, psoralidin, isobavachalcone, bavachinin, corylifol A, and bakuchiol, respectively. Nine of them were simultaneously quantitatively analyzed for the first time. A more comprehensive analytical method was established for the fingerprint of Fructus Psoraleae. It is very useful for authentication and quality assessment of the crude drug.

Production of bakuchiol by in vitro systems of Psoralea drupacea Bge

“…However, the aerial parts of P. drupacea grown in vitro are a potentially useful source of bakuchiol since these accumulate the compound up to a level of 11% on a dry weight basis. In planta Aerial part 6.5 a --Seeds -Up to 5.14 c -Concentration are expressed as % DW, -lack of data a Bondarenko et al (1977) b Abhyankar et al (2005) c Qiao et al (2007) …”

Recent analytical approaches in quality control of traditional Chinese medicines—A review

“…By this method, Psoralea corylifolia, Belamcanda chinensis and some TCM preparations, such as ShuangHuang-Lian oral liquid, Xuesetong injection, etc. were analyzed successfully [165][166][167][168][169].…”

Quality assessment of Chrysanthemum indicum Flower by simultaneous quantification of six major ingredients using a single reference standard combined with HPLC fingerprint analysis

“…Thus, it is reasonable and essential to combine multicomponent determination with fingerprint analysis to control the total quality of NDSs. However, the limited availability of various chemical standard substances for quantitative analysis is a major hurdle [11]. On assessing the alternative measures available for the practical comprehensive determination of NDSs, the single standard of determination multiple components (SSDMC) method is worth considering [12,13].Through the above analysis, it is necessary to perform the method based on SSDMC method and fingerprint analysis in routine quality control of CIF.…”