Chemical fingerprinting of <i>Equisetum arvense</i> L. using HPTLC densitometry and HPLC

Gallo, Francesca Romana; Multari, Giuseppina; Federici, E.; Palazzino, Giovanna; Giambenedetti, Massimo; Petitto, Valentina; Poli, Ferruccio; Nicoletti, Marcello

doi:10.1080/14786419.2011.558015

Cited by 29 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is interesting to note that previous phytochemical studies of Equisetum mostly focused their general antioxidant properties and on phenolic compounds, sterols and the silica content of the herb [33,34,37,43,44,70-76]. To the best of our knowledge, the role of inositol and choline in relation to the beneficial effects of E. arvense has not been investigated previously.…”

Section: Discussionmentioning

confidence: 99%

“…E. arvense is distributed worldwide over the northern hemisphere [32]. Equisetum species and hybrids are well understood to possess extensive morphological, morphometric and chemotypical variation [33,34] . E. arvense is used in traditional medicine as diuretic, anti-inflammatory, antioxidant, antidiabetic, vasorelaxant and hemostatic [35-41].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from America, China, Europe and India

et al. 2013

View full text Add to dashboard Cite

BackgroundPattern-oriented chemical profiling is increasingly being used to characterize the phytochemical composition of herbal medicines for quality control purposes. Ideally, a fingerprint of the biological effects should complement the chemical fingerprint. For ethical and practical reasons it is not possible to test each herbal extract in laboratory animals or humans. What is needed is a test system consisting of an organism with relevant biology and complexity that can serve as a surrogate in vitro system. The purpose of this study was to test the hypothesis that the Saccharomyces cerevisiae transcriptome might be used as an indicator of phytochemical variation of closely-related yet distinctly different extracts prepared from a single species of a phytogeographically widely distributed medicinal plant. We combined phytochemical profiling using chromatographic methods (HPTLC, HPLC-PDA-MS/MS) and gene expression studies using Affymetrix Yeast 2.0 gene chip with principal component analysis and k-nearest neighbor clustering analysis to test this hypothesis using extracts prepared from the phytogeographically widely distributed medicinal plant Equisetum arvense as a test case.ResultsWe found that the Equisetum arvense extracts exhibited qualitative and quantitative differences in their phytochemical composition grouped along their phytogeographical origin. Exposure of yeast to the extracts led to changes in gene expression that reflected both the similarities and differences in the phytochemical composition of the extracts. The Equisetum arvense extracts elicited changes in the expression of genes involved in mRNA translation, drug transport, metabolism of energy reserves, phospholipid metabolism, and the cellular stress response.ConclusionsOur data show that functional genomics in S. cerevisiae may be developed as a sensitive bioassay for the scientific investigation of the interplay between phytochemical composition and transcriptional effects of complex mixtures of chemical compounds. S. cerevisiae transcriptomics may also be developed for testing of mixtures of conventional drugs (“polypills”) to discover novel antagonistic or synergistic effects of those drug combinations.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from America, China, Europe and India

et al. 2013

View full text Add to dashboard Cite

show abstract

“…HPTLC, High Performance Thin Layer Chromatography, is the most recent evolution of planar chromatography and has been specifically tailored for analysis of natural products [14,15]. Typical product of the HPTLC is the fingerprint, as the individual chromatographic track representing, as near as possible, a mixture of organic substances [16,17]. Furthermore, the same plate can be visualized in several ways and individual spot quantitatively determined by densitometry.…”

Section: Towards Validationmentioning

confidence: 99%

HPTLC Fingerprint Analysis of Plant Staminal Cells Products

Nicoletti

Toniolo²

2012

J Chromat Separation Techniq

Self Cite

View full text Add to dashboard Cite

Utilization of natural products is radically changing. Changes were mainly due to the outcome in the market of a plethora of new food supplements, and in particular those generally named botanicals for their common plant origin. The validation of these novel products needs powerful analytical devices tailored for the study of herbal extracts in order to assess composition and face their natural complexity as a resource. The last item is important and crucial for the capacity and utility of the analytical results that means that each product should be analyzed with the right approach. Having in mind these arguments, we selected HPTLC as useful tool for the analysis of products based on plant staminal (stem) cells. Nowadays these products, generally named bud-derivatives, are waiting scientific validation to obtain their own place into food supplements regulation, after gained that in the market. Our analyses, based on HPTLC fingerprints, were able to show bud-derivatives complex compositions that resulted very similar, but also in part different, to those of the corresponding leaf hydro-alcoholic extract.

show abstract

“…Quality of henna dye is ranges from orange, auburn to burgundy. Lawsone occurs in the form of a glycosidic precursor that proficiently split the glycosidic bond when applied onto skin (Al-Tufail et al 1999;Cartwright-Jones 2006;Gallo et al 2008). Currently, numerous henna based formulations of natural dyes are available in the market.…”

Section: Introductionmentioning

confidence: 99%

An improved micropropagation and ex vitro rooting of a commercially important crop Henna (Lawsonia inermis L.)

Shiji

Siril

2018

Physiol Mol Biol Plants

View full text Add to dashboard Cite

An improved micropropagation protocol has been developed for a cosmetically important, dye yielding crop, henna (Lawsonia inermis). Quality of henna product is governed by naphthoquinone based pigment lawsone, thus in vitro multiplication of superior healthy plant to achieve enhanced productivity in terms of dye content and biomass deserve due attention. In the present study, nodal explants collected from an elite plant screened on the basis of superiority in lawsone content was cultured on MS medium supplemented with 0.5 lM benzyl adenine (BA) gave significantly (p \ 0.05) high number of shoots (24.33). The explants placed on MS medium augmented with 0.5 lM BA and 2-isopentenyladenine (2-iP) resulted in the formation of maximum number of shoots (43.67) and was elongated (12.57 cm) within 4 weeks of culture period. Enhanced axillary bud proliferation and production of mass number of micro shoots was achieved by the continuous subculture in MS medium containing 0.5 lM BA and 2-iP. In vitro raised micro shoots were dipped in 0.44 mM NAA for 5 min followed by planting in polyethylene pots containing a soil: vermiculite (1:1 v/v) mixture produced rooted plantlets (100%). Different auxin types and its concentrations had significant role rooting of L. inermis. Rooting response of various size shoots of L. inermis treated with 0.44 mM NAA showed 100% rooting in 4.1-5 cm size class shoots. After two months of potting, survived (95%) plants were successfully transferred to medicinal plant garden of the Department. The lawsone content of one-year-old micropropagated plants (23.04 mg/ g dw) growing in normal environmental conditions and elite mother plant (22.84 mg/g dw) was almost similar. Through the present study, efficient cloning of superior germplasm of L. inermis was established. Keywords Axillary bud Á Explant Á Ex vitro rooting Á Lawsonia inermis L. Á Micro-shoots Á MS medium

show abstract

Chemical fingerprinting of Equisetum arvense L. using HPTLC densitometry and HPLC

Cited by 29 publications

References 22 publications

The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from America, China, Europe and India

The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from America, China, Europe and India

HPTLC Fingerprint Analysis of Plant Staminal Cells Products

An improved micropropagation and ex vitro rooting of a commercially important crop Henna (Lawsonia inermis L.)

Contact Info

Product

Resources

About