CHEMICAL NATURE OF Α-Lipoic ACID

Reed, Lester J.; DeBusk, Betty G.; Gunsalus, I. C.; Schnakenberg, G. H.

doi:10.1021/ja01156a567

Cited by 39 publications

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“…faecalis as described by Korkes et al (1951). The sensitivity of both the oxidation and dismutation systems to inhibition by arsenite is consistent with a requirement for a-lipoic acid (Reed et al 1951;Moyed and O'Kane 1952b).…”

Section: Discussionsupporting

confidence: 60%

The Breakdown of Pyruvate by Asterococcus Mycoides, the Organism of Bovine Pleuropneumonia

Rodwell¹,

Rodwell²

1954

Aust. Jnl. Of Bio. Sci.

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Section: Discussionsupporting

confidence: 60%

The Breakdown of Pyruvate by Asterococcus Mycoides, the Organism of Bovine Pleuropneumonia

Rodwell¹,

Rodwell²

1954

Aust. Jnl. Of Bio. Sci.

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“…Similar properties. Recent reports on the properties of the acetate replacing factor (Reed et al, 1951b(Reed et al, , 1951c, pyruvic oxidase (Reed et al, 1951a(Reed et al, , 1951d, and protogen (Pierce et al, 1951) have disclosed marked similarities in properties to those previously reported for the B.R. factor (Kline and Barker, 1950).…”

mentioning

confidence: 78%

“…Since the activity was extractable with organic solvents from acidic solution (pH 1), it did not appear to contain a basic amino group. The highly purified or crystalline preparations of Reed and collaborators have also now been reported as acidic substances with pK values of 4.7 and 4.8 (Reed et al, 1951a(Reed et al, , 1951d). Their preparations are similarly extractable with organic solvents from strongly acid solutions (Reed et al, 1951c).…”

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confidence: 96%

“…These workers also found that liver contained a microbiologically inactive form or forms of acetate replacing factor. It appears significant that both Reed and collaborators and the Lederle group have found that highly purified concentrates of acetate replacing factor and protogen, respectively, contain two principal components, the less polar of which is converted to the more polar during partition manipulation (Reed et al, 1951b(Reed et al, , 1951dPierce et al, 1951).…”

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confidence: 99%

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Probable Identity of the Growth Promoting Factor for Butyribacterium Rettgeri With Other Biologically-Active Substances

et al. 1952

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Kline and Barker (1950) reported the existence and some properties of an apparently new unidentified growth factor (B.R. factor) required by Butyribacterium rettgeri for growth on lactate as the fermentable carbon source. Certain similarities in properties were given as the basis for a postulated relationship of the B.R. factor to other unidentified growth or metabolically active substances. These related factors included: (a) the acetate replacing factor reported by Guirard, Snell, and Williams (1946) as stimulatory for the early growth of Lactobacillus casei; (b) the pyruvic oxidase factor reported by O'Kane and Gunsalus (1947, 1948) as necessary for activation of the apo-pyruvic oxidase of Streptococcus faecalis; and (c) a factor necessary for the growth of Tetrahymena geleii caled protogen by Stokstad et al. (1949) who reported some of its properties. Stokstad et al. (1950) later reported that protogen was also necessary for the growth of an unidentified C(nebacterium. Snell and Broquist (1949) presented evidence for the interchangeability of these three latter activities. They reported that concentrates, purified with respect to pyruvic oxidase activity and having activities of 1, 70, and 3,000 pyruvic oxidase units per mg, had relative acetate replacing activity for L. cacsei of 1, 90, and 3,300, respectively. They also showed that a protogen preparation purified 10,000-fold over the source material as indicated by Tetrahymena assay was highly active for L. casei; 3 mug of this preparation were equivalent to the growth promoting activity of 400,ug of sodium acetate. The purpose of this report is to present further evidence on the relation of the B.R. factor to the three activities previously related by Snell and Broquist (1949). METHODS The methods employed in this work were the same as those previously described (Kline and Barker, 1950). EXPERIMENTAL RESULTS AND DISCUSSION Activity of pyrvic oxidase and acetate replacing preparation for B. rettgeri. Three pyruvic oxidase preparations with graded levels of activity, obtained 1 This investigation was supported in part by a research grant from the Division of

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“…(acetaldehyde), + POF (acetaldehyde)3 4b. (acetaldehyde)s + CoA (acetaldehyde)2 + POF In view of the importance of various sulfur compounds in carbonyl oxidation discussed by Korkes et al (1951), the demonstration that a-lipoic acid, one of the forms of pyruvate oxidation factor, is a sulfur compound (Reed et al, 1951) lends support to this hypothesis.…”

Section: Discussionmentioning

confidence: 97%