Chemical Tests for Identification and Characterization of Tomato Cultivars

Vishwanath, K.; Pallavi, H.M.; Nethra, N.; Prasad, S. Rajendra

doi:10.2478/v10129-011-0076-0

Cited by 6 publications

(8 citation statements)

References 3 publications

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“…Walls ( 1965 ), reported that the phenol colour reaction depends on the quality and quantity of oxidases present in the seeds, whereas Takahashi and Hamza ( 1983 ), reported that monophenol oxidase was extremely localized in aleurone layer of grains even though it is present in all other plant parts of a variety. Presence of enzyme system has been utilized to distinguish the crop varieties in rice and tomato (Pieper 1922 ; Joshi and Banerjee 1970 ; Mansing 2010 ; Vijayalakshmi and Vijay 2009 ; Anitalakshmi et al 2014 ; Sripunitha and Sivasubramaniam 2014 ; Vishwanath et al 2013 ).…”

Section: Resultsmentioning

confidence: 99%

Delineation of Inheritance Pattern of Aleurone Layer Colour Through Chemical Tests in Rice

Singh

Sripathy

Kumar

et al. 2017

Rice

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BackgroundRice aleurone layer develops different colours with various chemical tests that may help to develop some rapid tests for identification/grouping of rice varieties. Understanding the colour inheritance pattern could enable to develop chemical clues that may help for genetic purity analysis along with grow-out-test.ResultsIn this study, inheritance pattern of aleurone layer colour was studied in parents, F1 and F2 progenies derived from the crosses IR 36 × Acc. No. 2693 and IR 64 × Acc. No. 2693. The parent IR 36 showed light yellow (NaOH/KOH) and brown (phenol/modified phenol test) colour; whereas, Acc. No. 2693 revealed wine red/dark wine red (NaOH/KOH) and light brown colour/no reaction (phenol/modified phenol test). In contrary, another parent IR 64 exhibited light yellow (KOH/NaOH) and dark brown (phenol, modified phenol) colour. Both the F1 showed an intermediate light wine red colour (NaOH/KOH) and dark brown (phenol and modified phenol) colour, which is dominant over their one of the parents. The colour pattern with standard phenol/modified phenol, NaOH and KOH tests in F2 progenies of both the crosses showed 9:7 (complementary gene interaction) and 11:5 ratios (reciprocal dominance modification of recessive alleles), respectively.ConclusionsOur findings clearly elucidate the colour inheritance pattern in rice that may facilitate to develop rapid chemical tests to identify/ group the varieties for genetic purity analysis.

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Section: Resultsmentioning

confidence: 99%

Delineation of Inheritance Pattern of Aleurone Layer Colour Through Chemical Tests in Rice

Singh

Sripathy

Kumar

et al. 2017

Rice

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“…These tests were similar to observations in soya bean (Roopa et. al., 2008) and tomato (Vishwanath et. al., 2013).…”

Section: Seedling Growth Response To Kinetinmentioning

confidence: 99%

“…Similarly, in the case of Tomato, chemical tests are used to identify and characterize different cultivars. Though no single chemical test could distinguish all the varieties distinguishable chemical characters were used to develop the keys for the identification of each cultivar (Vishwanath et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Faster Chemical Methods to Determine Genetic Purity in Pigeon Pea (Cajanus cajan (L.) Mill sp.)

Mathad¹,

Channaveeraswamy²,

Kumar³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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The present investigation was carried out to find faster chemical methods to determine genetic purity in ten pigeon pea varieties. The faster methods are Ferrous Sulphate test, Sodium hydroxide test, peroxidase test, seedling growth response to GA 3, kinetin and 2-4D. Histogram using pixel luminance in ferrous sulphate test based on the grey scale produces values ranging from 17377 to 90616 which can be used to distinguish pigeon pea varieties. Similarly, sodium hydroxide test produced six coloured palette based on RGB values can distinguish varieties in pigeon pea. Also in peroxidase test, values for all ten pigeon pea varieties based on absorbance (0.252-2.372), peroxidase enzyme activity (0.791-7.442 IU/mg) and peroxidase enzyme volume (1.581-14.883 U/g) can be used for distinguishing varieties. Among the seedling growth response tests, in GA 3 test two varieties Asha (-2) and GC-11-39 (-6) were not responded and were recorded lowest values than the control. But remaining varieties can be distinguished as medium to high response types. Also in kinetin test two varieties not responded (GRG-811 and Asha) and all the other varieties can be distinguished based on this test. In case of 2-4D test all the varieties were recorded as susceptible. The co-efficient of variation for all the three tests based on the increase in coleoptile length over the control was ranged from 0.20 (Kinetin test) to 0.37 (GA 3 test).

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“…Accurate identification of varieties is not only a pre requisite for DUS testing, but is critical for the production of quality seed also. The most common method of varietal identification is grow out test [3,4], which is based on the morphological markers at various growth stages of plant, however it has many disadvantages such as interaction with environment, high cost and lengthy time required, low polymorphism in closely related varieties which were bred by using elite lines". "The time duration of one complete season for identification of varieties using standard grow-out test is one of the major constraints in its implementation, particularly in the major rice growing regions of North Western Himalayas.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas

Kumari¹,

Maharana²,

Aditya³

et al. 2023

IJECC

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Variety identification has great significance from seed production, breeding as well as intellectual property rights point of view to ensure quality seed. The decrease in the morphological variation among the major rice varieties due to narrow genetic base makes it necessary for the development of quick and reliable tests for varietal identification particularly for those working in seed certification and quality maintenance. The study was conducted during 2019-20 at the laboratories of the Crop Improvement Division, ICAR-VPKAS, Almora. Two hundred Indica rice varieties/genotypes were identified on the basis of seed color (phenol, modified phenol, NaOH and KOH) to chemical tests. Phenol test grouped variety into three distinct groups viz., dark brown (15 varieties), brown (59 varieties) and light brown (95 varieties). With the help of modified phenol test these varieties were further sub grouped. NaOH test grouped varieties into two distinct groups i.e., light yellow (177 varieties) and wine red (23 varieties). These tests clearly differentiated the varieties of one group to that of another groups on the basis of seed coat color. However, KOH test was not reliable for the differentiation of these varieties because all these varieties showed light yellow color after treating with KOH solution. Though no individual chemical test was able to distinguish all the varieties, different chemical tests in conjunction were useful in identification of varieties. Thus these simple, reliable and quick tests can be used for varietal identification in rice crop.

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Chemical Tests for Identification and Characterization of Tomato Cultivars

Cited by 6 publications

References 3 publications

Delineation of Inheritance Pattern of Aleurone Layer Colour Through Chemical Tests in Rice

Delineation of Inheritance Pattern of Aleurone Layer Colour Through Chemical Tests in Rice

Faster Chemical Methods to Determine Genetic Purity in Pigeon Pea (Cajanus cajan (L.) Mill sp.)

Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas

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