Chemically modified nylons as supports for enzyme immobilization

Urease was bound to commercially available nonwoven nylon fabric filters. Multilayer immobilized-enzyme filter reactors were constructed by packing varying numbers of urease-nylon filters in a column. Owing to the relatively open structure and high mechanical strength of the filter fabric, compaction and pressure drop effects were minimal. The reactors could be operated in a wide range of substrate concentrations and flow rates under conditions where mass-transfer limitations could be neglected. The kinetic behavior of the immobilized-enzyme filter reactors could be described by a linear form of the integrated Michaelis-Menten equation using a model based on the sequential action of the enzyme filters.

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Immobilization of microbial cells in crosslinked, prepolymerized, linear polyacrylamide gels: Antibiotic production by immobilized Streptomyces clavuligerus cells

Freeman

Aharonowitz

1981

Biotech & Bioengineering

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SummaryA mild new method for the immobilization of whole microbial cells has been developed. Cells were suspended in a solution of preformed, linear, water-soluble polyacrylamide chains, partially substituted with acylhydrazide groups. The prepolymerized backbone polymer was crosslinked, in the presence of viable cells, by stoichiometric amounts of dialdehydes such as glyoxal, glutardialdehyde, and periodate-oxidized polyvinyl alcohol. The crosslinking reaction, carried out in cold, neutral physiological conditions resulted in cells entrapped in gels with physical properties similar to those of the common polyacrylamide gels. However, cell damage generally caused by the acrylamide monomer was avoided. Resting Streptomyces clavuligerus cells, possessing a high capacity for antibiotic production, were entrapped according to this procedure. These immobilized cells produced cephalosporins continuously for 96 h with yields similar to those of free resting cells. The same cells, when immobilized by direct polymerization of acrylamide monomers, yielded significantly lower amounts of antibiotics.

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Chemically modified nylons as supports for enzyme immobilization

Cited by 18 publications

References 17 publications

Chemically modified polyesters as supports for enzyme immobilization: Isocyanide, acylhydrazide, and aminoaryl derivatives of poly(ethylene terephthalate)

Chemically modified polyesters as supports for enzyme immobilization: Isocyanide, acylhydrazide, and aminoaryl derivatives of poly(ethylene terephthalate)

Multilayer immobilized‐enzyme filter reactors: Urease bound to nylon fabric filters

Immobilization of microbial cells in crosslinked, prepolymerized, linear polyacrylamide gels: Antibiotic production by immobilized Streptomyces clavuligerus cells

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