Chemically Modified Polymers Containing Isocyanide Functional Groups as Supports for Enzyme Immobilization

Goldstein, Léon; Freeman, Amihay; Blassberger, D.; Granot, R.; Sokolovsky, Mordechai

doi:10.1016/b978-0-12-110950-9.50017-3

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…After hydrolysis using HCl, an enzyme may be coupled to the carrier through cross-linking with glutaraldehyde [2-71. The chemical groups in peptide bonds can be modified by 0-alkylation [&11] or N-alkylation [12,131. Enzymes can also be adsorbed on synthetic anionite AQ-nylon [14, 151.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of glucoamylase on polyamide nets

Miller

1998

Acta Biotechnologica

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The formation of reactive groups on polyamide nets (nylon 6) and the subsequent immobilization of glucoamylase were investigated. Different mesh sizes of the nets and two chemical methods of enzyme coupling -i) partial hydrolysis of the polyamide with subsequent glutaraldehyde binding and ii) 0-alkylation of the carrier using a treatment with a benzene-methyl sulphate mixture -were usedThe reactivity of immobilized glucoamylase (GA) was tested by hydrolysis reactions using 1% starch solutions. The highest reactivity (140 pg glc/(min x cm2) was obtained for methylated nylon samples attached to a glass rod and by coupling glucoamylase on the nylon surface which had been treated with lysine and glutaraldehyde. This method resulted in a more reactive and more stable preparation of immobilized glucoamylase as compared to a simpler method of coupling glutaraldehyde to partially hydrolyzed nylon.

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Section: Introductionmentioning

confidence: 99%